Both Arabidopsis TATA binding protein (TBP) isoforms are functionally identical in RNA polymerase II and III transcription in plant cells: evidence for gene-specific changes in DNA binding specificity of TBP.

Heard, David J.; Kiss, Tamás; Filipowicz, Witold

doi:10.1002/j.1460-2075.1993.tb06026.x

Cited by 31 publications

(23 citation statements)

References 64 publications

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“…2C). Mutations in the TATA box element shown previously to inactivate U6 gene expression in plant protoplasts (26,27) also caused 7SL gene activity to drop to virtually undetectable levels (TATA-Dn; Fig 2B, lane 3). Mutation of the USE and TATA elements simultaneously produced a similar effect to the TATA mutant alone (USE+TATA-Dn; Fig.…”

Section: Sequence Analysismentioning

confidence: 55%

“…Mutation of the TATA box results in complete inactivation of the gene in transfected protoplasts. The same mutation was previously shown to abolish Arabidopsis U6 gene transcription in vivo (26,27). While the point mutations made in the USE sequence of At7SL-2 were not previously tested in pol Ill-specific U-snRNA genes, it has been observed that a different point mutation in the USE had similar effects on both pol II and pol III U-snRNA transcription in plant protoplasts (33).…”

Section: -Gsmentioning

confidence: 86%

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An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

Heard

Filipowicz

Marques³

et al. 1995

Nucl Acids Res

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The genes transcribed by RNA polymerase (pol)111 can be placed into four distinct groups based on the nature and position of their promoter elements. In the higher eukaryotes equivalent genes usually belong to the same sub-type of pol Hi promoters and there are few examples of genes which have changed promoter type during evolution. In this work we demonstrate that the promoter of the Arabidopsis thaliana 7SL RNA gene is located upstream of the coding region and is identical to the promoters of pol III-specific plant U-small nuclear RNA (U-snRNA) genes. Sequence analysis of two different 7SL genes from A.thaliana revealed that both genes contain two sequence elements in their 5' flanking regions identical in sequence and position to the highly conserved USE and TATA elements of the pol III-transcribed plant U-snRNA genes. Mutational analysis of these elements in the At7SL-2 gene indicates that the USE and TATA elements are both necessary and account for >90°% of the transcriptional activity of this gene in transfected plant protoplasts. Within the coding region of both genes there is a sequence element which is a 10/11 nt match to the consensus B-box element of tRNA genes, however, this element is not important for gene activity. These findings distinguish the plant genes from the human 7SL gene, which has both intemal and upstream promoter elements and its upstream elements are different from those found in the human U-snRNA genes.

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Section: Sequence Analysismentioning

confidence: 55%

Section: -Gsmentioning

confidence: 86%

An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

Heard

Filipowicz

Marques³

et al. 1995

Nucl Acids Res

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“…This TBP derivative (TBP^g) has a relaxed DNA-binding spec ificity and recognizes both a canonical TAT AAA box and an altered TGTAAA box (Strubin and Struhl 1992). The combination of TEP^s and the TGTAAA box circum vents the activity of endogenous wild-type TBP in yeast (Strubin and Struhl 1992), plant (Heard et al 1993), and mammalian (Keaveney et al 1993) cells and thus allows the effects of in vitro manipulations in TBP to be exam ined in vivo.…”

Section: Experimental Designmentioning

confidence: 99%

Multiple regions of TBP participate in the response to transcriptional activators in vivo.

Tansey

Ruppert²,

Tjian³

et al. 1994

Genes Dev.

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We used mutant yeast and human TBP molecules with an altered DNA-binding specificity to examine the role of TBP in transcriptional activation in vivo. We show that yeast TBP is functionally equivalent to human TBP for response to numerous transcriptional activators in human cells, including those that do not function in yeast. Despite the extensive conservation of TBP, its ability to respond to transcriptional activators in vivo is curiously resistant to clustered sets of alanine substitution mutations in different regions of the protein, including those that disrupt DNA binding and basal transcription in vitro. Combined sets of these mutations, however, can attenuate the in vivo activity of TBP and can differentially affect response to different activation domains. Although the activity of TBP mutants in vivo did not correlate with DNA binding or basal transcription in vitro, it did correlate with binding in vitro to the largest subunit of TFIID, hTAFnZSO. Together, these data suggest that TBP utilizes multiple interactions across its surface to respond to RNA polymerase II transcriptional activators in vivo; some of these interactions appear to involve recruitment of TBP into TFIID, whereas others are involved in response to specific types of transcriptional activators.

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“…Mot1 associates with Pol III promoters, although the Mot1/ TBP occupancy ratio is low. Although biochemical analysis indicates that repression by Mot1 is specific for Pol II promoters (4), Pol III promoters often have consensus TATA elements that affect transcription via the specific DNA-binding activity of TBP (19,54). Interestingly, Mot1 associates with the SNR6 and five tRNA promoters at a level that is comparable to that observed at FBA1, a strong Pol II promoter (Fig.…”

mentioning

confidence: 96%

Mot1 Associates with Transcriptionally Active Promoters and Inhibits Association of NC2 in Saccharomyces cerevisiae

Geisberg

Moqtaderi

Kuras

et al. 2002

Molecular and Cellular Biology

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Mot1 stably associates with the TATA-binding protein (TBP), and it can dissociate TBP from DNA in an ATP-dependent manner. Mot1 acts as a negative regulator of TBP function in vitro, but genome-wide transcriptional profiling suggests that Mot1 positively affects about 10% of yeast genes and negatively affects about 5%. Unexpectedly, Mot1 associates with active RNA polymerase (Pol) II and III promoters, and it is rapidly recruited in response to activator proteins. At Pol II promoters, Mot1 association requires TBP and is strongly correlated with the level of TBP occupancy. However, the Mot1/TBP occupancy ratio at both Mot1-stimulated and Mot1-inhibited promoters is high relative to that at typical promoters, strongly suggesting that Mot1 directly affects transcriptional activity in a positive or negative manner, depending on the gene. The effect of Mot1 at the HIS3 promoter region depends on the functional quality and DNA sequence of the TATA element. Unlike TBP, Mot1 association is largely independent of the Srb4 component of Pol II holoenzyme, and it also can occur downstream of the promoter region. Mot1 removes TBP, but not TBP complexes or preinitiation complexes, from inappropriate genomic locations. Mot1 inhibits the association of NC2 with promoters, suggesting that the TBP-Mot1 and TBP-NC2 complexes compete for promoter occupancy in vivo. We speculate that Mot1 does not form transcriptionally active TBP complexes but rather regulates transcription in vivo by modulating the activity of free TBP and/or by affecting promoter DNA structure.The TATA-binding protein (TBP) is the central initiation factor for transcription by all three nuclear RNA polymerases (Pol). TBP is a component of the SL-1, TFIID, and TFIIIB complexes that mediate transcription by Pol I, Pol II, and Pol III, respectively (20,46). With respect to Pol II transcription in yeast cells, TBP is essential for transcription of all genes (9), whereas the associated factors (TAFs) within the TFIID complex are selectively required (21,37,38,45,52,53). Relative to TBP occupancy, TAF association is high at TAF-dependent promoters and is low at TAF-independent promoters, indicating that Saccharomyces cerevisiae has at least two forms of transcriptionally active TBP in vivo (27,32). The TFIID form is recruited to ribosomal protein promoters by a Rap1-containing activator (35), and it is important at several promoters with weak TATA elements (35,37,38,49). The TAF-independent form(s) of TBP predominates at many strong promoters, and it is preferentially recruited to promoters by most yeast activators (27,32,35). It is unclear whether the TAF-independent form(s) is TBP itself, which is present in yeast cell extracts (18

show abstract

Both Arabidopsis TATA binding protein (TBP) isoforms are functionally identical in RNA polymerase II and III transcription in plant cells: evidence for gene-specific changes in DNA binding specificity of TBP.

Cited by 31 publications

References 64 publications

An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

An upstream U-snRNA gene-like promoter is required for transcription of theArabidopsis thaliana7SL RNA gene

Multiple regions of TBP participate in the response to transcriptional activators in vivo.

Mot1 Associates with Transcriptionally Active Promoters and Inhibits Association of NC2 in Saccharomyces cerevisiae

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