Botanical origin and antioxidant activities of propolis from the Irano-Turanian region

Abstract

Propolis is a natural bioactive mix and a traditional medicine that has been used for treating several complications. The bioactive properties of propolis are dependent on its botanical origin. This study investigated the pollen composition, antioxidant activities, and the total phenol and total flavonoid content of a propolis sample from the Refahiye (Erzincan, Turkey) region. Melissopalynological analysis conducted according to the relevant literature revealed that the pollen profile of the sample primarily indicated the presence of the Fabaceae (38.4%), Asteraceae (20.2%), and Fagaceae (11.2%) families. The antioxidant ability of propolis extract was analyzed by the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50), ferric reducing antioxidant power capacity (FRAP) (%), DPPH radical scavenging activity (in terms of SC50), metal-chelating activity (%), total phenol content (TPC), and total flavonoid content (TFC), which showed the following values: 11.72±0.04 µg/mL, 90.73% ± 0.24%, 18.34±0.08 µg/mL, 89.69% ± 0.12%, 10673.4±3.30 mg GAE/100 g of propolis sample (PS), and 170.65±1.12 mg QE/100 g of PS, respectively. These results were compared using butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and α-tocopherol (TOC) as standard antioxidant compounds. The high biological activity of propolis from the Refahiye region could be attributed to its rich pollen composition. These results indicate that propolis is an important source in terms of its antioxidant activities.

Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical -Properties and Mineral Profiles

“…Ascorbic acid also had a higher scavenging capacity than Asela and Gambela propolis. This finding was in agreement with the report of Turkish propolis (18.34 ± 0.08 μg/ml) [68], and higher than the IC 50 obtained from Malaysian stingless bee propolis (15-270 μg/ml) [58] and Korean propolis (43 and 269 μg/ml). The variation in scavenging activity could be due to their resin plant origin, climate, and resin collection time by the bees [30].…”

“…There was no significant difference (p > 0.05) in the H 2 O 2 values between Asela, Sheka, Gambela propolis and standard ascorbic acids %inhibition (H 2 O 2 ). The percentage inhibition of H 2 O 2 of this finding was higher than the Turkish propolis (11.72 ± 0.04 μg/ml) [68] and lower than the Egyptian propolis (67%-79.25%) [69].…”

Helichrysum armenium DC. subsp. araxinum (Kirp.) Takht. taksonunun bazı biyokimyasal içeriklerinin belirlenmesi

“…Ferrous ion chelating activity (%) = [1 -(As /Ac )] × 100 where Ac was the absorbance of the control, and As was the absorbance of the extract/ standard [24]. Positive control was Na2EDTA.…”

Palynological Analyses of Malatya Propolis Samples

“…Because the propolis biochemical content is directly related with plants that visited by bees. Either our country or around the world there are many melissopalynological researches on propolis (Park et al 2002;Gencay Çelemli and Sorkun 2012;Popova et al 2013;Bayram 2015;Kızılpınar et al 2017;Tatlısulu and Özgör 2023). This preliminary study was carried out for contribute the former researches on this subject and lead beekeepers to choose valid areas to place their beehives.…”