Boosting sericin extraction through alternative silk sources

Jaramillo‐Quiceno, Natalia; Callone, Emanuela; Dirè, Sandra; Álvarez‐López, Catalina; Motta, Antonella

doi:10.1038/s41428-021-00539-2

Cited by 10 publications

(8 citation statements)

References 68 publications

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“…Cocoons (10 g) were cut into pieces, washed with deionized water, immersed in 0.02 M Na 2 CO 3 solution (200 mL), and boiled for 1 h. The silk fibroin was removed by filtration, and the supernatant was dialyzed against deionized water for Na 2 CO 3 removal for 4 days at room temperature. 25,26 2.3.5 | Alkali and high temperature-high pressure degumming method Cocoons (10 g) were processed as above-mentioned in 0.02 M Na 2 CO 3 solution (200 mL) and then autoclaved for 1 h at 120 C and 1.5 lb/in 2 . The silk fibroin was removed by filtration, and the supernatant was dialyzed against deionized water to remove Na 2 CO 3 for 4 days at room temperature.…”

Section: Alkali and High Temperature Degumming Methodsmentioning

confidence: 99%

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Promising growth inhibitory potential of extracted natural silk sericin biopolymer against DEN‐induced liver tumor in male mice

Bakr,

Sadek,

El‐Samad

et al. 2024

J of Applied Polymer Sci

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Silk sericin (SS), a protein biopolymer, is gaining an increasing attention in a variety of biomedical applications. Our objective was to identify the optimal extraction method yielding the highest silk protein content, to assess its chemical elemental composition, to characterize it, and to compare its conformation by X‐ray diffraction and Fourier‐transform infrared versus that of the commercial one. Herein, alkali and high temperature is the perfect approach. This study aimed further to test whether extracted SS could attenuate the adverse hepatotoxic effects induced by diethylnitrosamine (DEN) in mice. Animals were injected intraperitoneally once weekly for four consecutive weeks with physiological saline (0.9% NaCl), DEN (25 mg/kg), SS (1 g/kg) 90 min prior to DEN injection, melatonin (10 mg/kg) followed within 1 h with the administration of SS and 30 min later with DEN. The results confirmed the hepatotoxicity of DEN manifested by an increase in liver enzymes' activity, and pathological changes in the liver tissue. The combined use of SS and/or melatonin with DEN returned most of the parameters to values similar to the controls. Briefly, chemically characterized SS seems a valuable extract for further consideration as anti‐carcinogenic agent. It has the potential to upgrade liver cancer chemotherapeutic agents.

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Section: Alkali and High Temperature Degumming Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Promising growth inhibitory potential of extracted natural silk sericin biopolymer against DEN‐induced liver tumor in male mice

Bakr,

Sadek,

El‐Samad

et al. 2024

J of Applied Polymer Sci

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“…Among the proteins with the potential for the development of hydrogels is silk sericin (SS), composed mostly of polar amino acids with carboxyl and hydroxyl side chains [27]. This composition gives it a high water absorption capacity [28] and good barrier properties. Moreover, SS is thermally stable below 200 • C and exhibits a T g between 170 • C and 197 • C [29,30].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a New Silk Sericin-Based Hydrogel for Water Retention in Soil

et al. 2023

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Hydrogel-type absorbent materials are currently a technological alternative for improving water retention in the soil and reducing nutrient loss by leaching and evaporation. This study aimed to evaluate the application of a new hydrogel based on silk sericin (SS) as a water retention material in soil. The morphology of the hydrogel was characterized using Scanning Electron Microscopy (SEM), and its impact on moisture retention in sandy loam soil (SLS) under different levels of matric pressure (MP) was evaluated. Additionally, water content data were collected over time for both SLS and SLS with hydrogel (SLS + H), and the data were used to fit predictive models. The results indicate that the hydrogel had a porous morphology that promoted water retention and soil release. Under a MP of 0.3 bar, the use of the hydrogel increased water retention by 44.70% with respect to that of SLS. The predictive models developed were adequately adjusted to the behavior of the moisture data over time and evidenced the incidence of the absorbent material on the dynamics of the moisture content in the soil. Therefore, these models could be useful for facilitating subsequent simulations or for designing automatic soil moisture control systems oriented to smart farming.

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“…To extract and use sericin from the cocoon, different methods can be chosen: high temperature (with or without high pressure) by autoclaving, acidic solution (citric, tartaric, succinic acid), soap, and alkali solutions (sodium carbonate), highly concentrated urea or by enzymatic processes 52–54 . With different extraction protocols, the chemical structure of sericin and its amino acid composition changes, which could impact the potential biomedical application 54–56 . The degumming process is also fundamental for the extraction and regeneration of silk fibroin into an aqueous solution and for the structural integrity of the three subunits of the silk fibroin protein complex 57 .…”

Section: Introductionmentioning

confidence: 99%

“… 52 , 53 , 54 With different extraction protocols, the chemical structure of sericin and its amino acid composition changes, which could impact the potential biomedical application. 54 , 55 , 56 The degumming process is also fundamental for the extraction and regeneration of silk fibroin into an aqueous solution and for the structural integrity of the three subunits of the silk fibroin protein complex. 57 Thus, it is often a crucial first step for developing silk‐based biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Biomedical applications of silk and its role for intervertebral disc repair

et al. 2022

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Intervertebral disc (IVD) degeneration (IDD) is the main contributor to chronic low back pain. To date, the present therapies mainly focus on treating the symptoms caused by IDD rather than addressing the problem itself. For this reason, researchers have searched for a suitable biomaterial to repair and/or regenerate the IVD. A promising candidate to fill this gap is silk, which has already been used as a biomaterial for many years. Therefore, this review aims first to elaborate on the different origins from which silk is harvested, the individual composition, and the characteristics of each silk type. Another goal is to enlighten why silk is so suitable as a biomaterial, discuss its functionalization, and how it could be used for tissue engineering purposes.The second part of this review aims to provide an overview of preclinical studies using silk-based biomaterials to repair the inner region of the IVD, the nucleus pulposus (NP), and the IVD's outer area, the annulus fibrosus (AF). Since the NP and the AF differ fundamentally in their structure, different therapeutic approaches are required.Consequently, silk-containing hydrogels have been used mainly to repair the NP, and silk-based scaffolds have been used for the AF.

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Boosting sericin extraction through alternative silk sources

Cited by 10 publications

References 68 publications

Promising growth inhibitory potential of extracted natural silk sericin biopolymer against DEN‐induced liver tumor in male mice

Promising growth inhibitory potential of extracted natural silk sericin biopolymer against DEN‐induced liver tumor in male mice

Characterization of a New Silk Sericin-Based Hydrogel for Water Retention in Soil

Biomedical applications of silk and its role for intervertebral disc repair

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