Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

Ju, Jung-Hyun; Oh, Beak-Rock; Ko, Dongjin; Heo, Sun-Yeon; Lee, Jong-Jea; Kim, Young‐Min; Yang, KwonSeok; Seo, Jeong-Woo; Hong, Won-Kyung; Kim, Chul‐Ho

doi:10.1016/j.algal.2019.101474

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…SH 103 were DHA 34.96%, docosapentaenoic acid (DPA, C22:5n6) 9.12% and palmitic acid (C16:0) 49.84%. These values were slightly different from the results of Ju et al [24] in DPA and DHA contents, which was thought to be due to differences in the culture method and the culture time.…”

Section: Lipid and Faee Composition Of Schizochytrium Sp Sh 103contrasting

confidence: 99%

“…The microalgae used in this study was Schizochytrium sp. SH103 (KCTC 21861BP) donated by Microbiological research center, Korea research institute of bioscience and biotechnology (KRIBB) [24]. Seed culture was performed as follows; the cells were grown in a basal medium (60 g/L glucose, 10 g/L yeast extract, 9 g/L KH 2 PO 4 , 10 g/L sea salt, 2 mg/L tetracycline, vitamin mixture [thiamin 9.5 mg/L, biotin 0.2 mg/L and cyanocobalamin 1.0 mg/L]) for 2 days at 28 ℃ and 125 rpm on a rotary shaker.…”

Section: Microorganism and Sample Preparation Microalgae And Culture mentioning

confidence: 99%

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Purification of high purity docosahexaenoic acid from Schizochytrium sp. SH103 using preparative-scale HPLC

Kim²,

Park

et al. 2020

Appl Biol Chem

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High purity polyunsaturated fatty acids (> 95%) are essential for the synthesis of specialized pro-resolving lipid mediators (SPMs), such as protectins, resolvins, and maresins, which are used for clinical application. To date, high purity (> 95%) eicosapentaenoic acid (EPA; C20:5n3) and docosahexaenoic acid (DHA; C22:6n3) have been produced through various manufacturing steps using fish oil. In this study, we optimized preparative high performance liquid chromatography (HPLC) process to purify high-purity DHA ethyl ester (DHAee; > 98%) from oleaginous microalgae Shizochytrium sp. SH103 containing at least 34% DHA content. The purity and yield of DHA were determined by reverse phase chromatography with changing the mobile phase velocity, loading amount, and mobile phase composition. On a semi-preparative scale, optimal DHA separation in isocratic elution was obtained with a mobile phase velocity of 0.5 mL/min, a loading amount of 10 mg/mL, and mobile phase composition of methanol/water (96:4, v/v), wherein the purity of DHA was 98.5%. This separation was scaled up to a preparative column, resulting in 99.0% DHA fraction with a yield of 79.8%. This result suggests that a large amount of high purity DHA can be produced from microalgae when scaling up a preparative column to an industrial column.

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Section: Lipid and Faee Composition Of Schizochytrium Sp Sh 103contrasting

confidence: 99%

Section: Microorganism and Sample Preparation Microalgae And Culture mentioning

confidence: 99%

Purification of high purity docosahexaenoic acid from Schizochytrium sp. SH103 using preparative-scale HPLC

Kim²,

Park

et al. 2020

Appl Biol Chem

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“…DHA was obtained from Thraustochytrid microalgae ( Schizochytrium sp. SH103) 14 . The HFA standards 17 S -HDHA and resolvin D1-5 were obtained from Cayman Chemical (Ann Arbor, MI, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The DHA as substrate used in the reaction was obtained from Schizochytrium sp. SH103 14 , 16 . The reaction mixture was prepared so as to contain 50 μM substrate in 50 mM Tris–HCl buffer (pH 8.0), and the reaction was initiated by adding Osc-LOX at concentrations of 50–400 units mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Heo

et al. 2020

Sci Rep

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Omega-3 polyunsaturated fatty acids (PUFAs) have been known to have beneficial effects in the prevention of various diseases. Recently, it was identified that the bioactivities of omega-3 are related to lipid mediators, called pro-resolving lipid mediators (SPMs), converted from PUFAs, so they have attracted much attention as potential pharmaceutical targets. Here, we aimed to build an efficient production system composed of enzymatic and chemical catalysis that converts docosahexaenoic acid (DHA) into lipid mediators. The cyanobacterial lipoxygenase, named Osc-LOX, was identified and characterized, and the binding poses of enzyme and substrates were predicted by ligand docking simulation. DHA was converted into three lipid mediators, a 17S-hydroxy-DHA, a 7S,17S-dihydroxy-DHA (RvD5), and a 7S,15R-dihydroxy-16S,17S-epoxy-DPA (new type), by an enzymatic reaction and deoxygenation. Also, two lipid mediators, 7S,15R,16S,17S-tetrahydroxy-DPA (new type) and 7S,16R,17S-trihydroxy-DHA (RvD2), were generated from 7S,15R-dihydroxy-16S,17S-epoxy-DPA by a chemical reaction. Our study suggests that discovering new enzymes that have not been functionally characterized would be a powerful strategy for producing various lipid mediators. Also, this combination catalysis approach including biological and chemical reactions could be an effective production system for the manufacturing lipid mediators.

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“…Efficient fed-batch fermentation can increase the production of target bio-products [36,37]. Thus, we tested the effect of fed-batch fermentation on 1,3-PDO production by L. reuteri CH53 under optimized conditions (unaerated and 100 rpm) (Fig.…”

Section: Effect Of Fed-batch Fermentation On Production Of 13-pdomentioning

confidence: 99%

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Wang

Heo

et al. 2020

Microb Cell Fact

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Background: 1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO. Results:We identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites. Conclusions:We used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

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Boosting productivity of heterotrophic microalgae by efficient control of the oxygen transfer coefficient using a microbubble sparger

Cited by 22 publications

References 30 publications

Purification of high purity docosahexaenoic acid from Schizochytrium sp. SH103 using preparative-scale HPLC

Purification of high purity docosahexaenoic acid from Schizochytrium sp. SH103 using preparative-scale HPLC

Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

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