Boosting Enzyme Activity in Enzyme Metal–Organic Framework Composites

Abstract

Enzymes, as highly efficient biocatalysts, excel in catalyzing diverse reactions with exceptional activity and selective properties under mild conditions. Nonetheless, their broad applications are hindered by their inherent fragility, including low thermal stability, limited pH tolerance, and sensitivity to organic solvents and denaturants. Encapsulating enzymes within metal−organic frameworks (MOFs) can protect them from denaturation in these harsh environments. However, this often leads to a compromised enzyme activity. In recent years, extensive research efforts have been dedicated to enhancing enzymatic activity within MOFs, leading to the development of new enzyme− MOF composites that not only preserve their catalytic potential but also outperform their free counterparts. This Review provides a comprehensive review on recent developments in enzyme−MOF composites with a specific emphasis on their enhanced enzymatic activity compared to free enzymes.

Trametes versicolor Laccase-Based Magnetic Inorganic-Protein Hybrid Nanobiocatalyst for Efficient Decolorization of Dyes in the Presence of Inhibitors

“…Other limitations include the potential for enzyme inactivation due to interactions with the immobilization support or other nearby enzymes, as well as difficulties in achieving optimal enzyme loading. Further research is required to better understand these limitations and develop strategies to overcome them [11,13,14].…”