BoneSource? hydroxyapatite cement: A novel biomaterial for craniofacial skeletal tissue engineering and reconstruction

Friedman, Craig D.; Costantino, Peter D.; Takagi, Shōzō; Chow, Laurence C.

doi:10.1002/(sici)1097-4636(199824)43:4<428::aid-jbm10>3.0.co;2-0

Cited by 404 publications

(381 citation statements)

References 8 publications

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“…36,37 CPC was approved in 1996 by the Food and Drug Administration for repairing craniofacial defects, thus becoming the first CPC for clinical use. 38 Traditional CPC was brittle and weak; however, the mechanical properties of CPC were improved by incorporating chitosan. 39,40 The CPC-chitosan scaffold supported the adhesion and proliferation of osteoblast cells and hMSCs.…”

mentioning

confidence: 96%

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair

Chen

Zhou

Weir

et al. 2013

Tissue Engineering Part A

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Calcium phosphate cement (CPC) has in situ-setting ability and excellent osteoconductivity. Human embryonic stem cells (hESCs) are exciting for regenerative medicine due to their strong proliferative ability and multilineage differentiation capability. However, there has been no report on hESC seeding with CPC. The objectives of this study were to obtain hESC-derived mesenchymal stem cells (hESCd-MSCs), and to investigate hESCd-MSC proliferation and osteogenic differentiation on novel CPC with chitosan immobilized with RGD (CPC-chitosan-RGD). RGD was covalently bonded with chitosan, which was then incorporated into CPC. The CPC-chitosan-RGD scaffold had higher strength and toughness than CPC-chitosan control without RGD ( p < 0.05). hESCs were cultured to form embryoid bodies (EBs), and the MSCs were then migrated out of the EBs. Flow cytometry indicated that the hESCd-MSCs expressed typical surface antigen profile of MSCs. hESCd-MSCs had good viability when seeded on CPC scaffolds. The percentage of live cells and the cell density were significantly higher on CPC-chitosan-RGD than CPC-chitosan control. Scanning electron microscope examination showed hESCd-MSCs with a healthy spreading morphology adherent to CPC. hESCd-MSCs expressed high levels of osteogenic markers, including alkaline phosphatase, osteocalcin, collagen I, and Runx2. The mineral synthesis by the hESCd-MSCs on the CPC-chitosan-RGD scaffold was twice that for CPC-chitosan control. In conclusion, hESCs were successfully seeded on CPC scaffolds for bone tissue engineering. The hESCd-MSCs had good viability and osteogenic differentiation on the novel CPC-chitosan-RGD scaffold. RGD incorporation improved the strength and toughness of CPC, and greatly enhanced the hESCd-MSC attachment, proliferation, and bone mineral synthesis. Therefore, the hESCd-MSC-seeded CPC-chitosan-RGD construct is promising to improve bone regeneration in orthopedic and craniofacial applications.

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confidence: 96%

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair

Chen

Zhou

Weir

et al. 2013

Tissue Engineering Part A

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“…Hydroxyapatite: By insertion porous HA wedges into the tibias of ten knees in seven patients having high tibial osteotomies, it was found that pores located at the interface, at the time of hardware removal, were completely filled with bone, and the bone depth formed increased consistently with time [137]. In another study, the transverse sections of porous HA implants placed in rabbit tibias demonstrated a new bone growth through the pores.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Bioceramic Scaffolds

Amin¹,

Ewais²

2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Millions of peoples in the world suffer from their bone damage tissues by disease or trauma. Every day, thousands of surgical procedures are performed to replace or repair these tissues. The availability of these tissues is a big problem, and their costs are expensive. The repair of these defects has become a major clinical and socioeconomic need with the increase of aging population and social development. The emerge of tissue engineering (TE) is considered as a glimmer of hope to contribute in solving this problem. It aims at the regeneration of damaged tissues with restoring and maintaining the function of human bone tissues using the combination of cell biology, materials science, and engineering principles. In this chapter, the current state of the tissue engineering in particular bioceramic scaffolds was discussed. Concept of tissue engineering was explored. Bioceramic scaffold materials, their processing techniques, challenges taken into consideration the design of the scaffolds, and their in-vitro and in-vivo studies were highlighted. The scaffolds with extra-functionalities such as drug release ability and clinical applications were mentioned.

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“…Neben diesen beiden Komplikationen führte seine fehlende ossäre Integrierbarkeit [12] dazu, dass Zemente auf Ca-P-Basis (Ca-P: Kalziumphosphat) mehr in den Focus rückten. Diese haben den Vorteil, dass sie resorbierbar sind und zu eigener Knochenstruktur umgebaut werden [6]. Außerdem erfolgt die Aushärtung nur knapp über Körpertemperatur [14].…”

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