Bone substitute biomedical material of multi-(amino acid) copolymer: in vitro degradation and biocompatibility

Li, Hong; Yan, Yonggang; Wei, Jie; Ma, Jian; Gong, Min; Luo, Xiaoman; Zhang, Yunfei

doi:10.1007/s10856-011-4439-8

Cited by 32 publications

(37 citation statements)

References 25 publications

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“…This copolymer could be degraded in HCL-Tris solution with weight loss of about 30 wt% after 12 weeks of soaking. 16 In particular, our results show that during the degradation period, this copolymer had no significant effect on the pH of the ambient environment and maintained a complete structure and adequate mechanical strength compared with other degradation polymers, such as polylactic acid and it copolymers. Cell culture experiments and in vivo implantation results show that the copolymer also had good biocompatibility.…”

Section: Introductionmentioning

confidence: 67%

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

Hong

Gong²,

Yang³

et al. 2012

IJN

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Background and methods:A nano calcium-deficient hydroxyapatite (n-CDHA)-multi(amino acid) copolymer (MAC) composite bone substitute biomaterial was prepared using an in situ polymerization method. The composition, structure, and compressive strength of the composite was characterized, and the in vitro degradability in phosphate-buffered solution and preliminary cell responses to the composite were investigated. Results: The composite comprised n-CDHA and an amide linkage copolymer. The compressive strength of the composite was in the range of 88-129 MPa, varying with the amount of n-CDHA in the MAC (ranging from 10 wt% to 50 wt%). Weight loss from the composite increased (from 32.2 wt% to 44.3 wt%) with increasing n-CDHA content (from 10 wt% to 40 wt%) in the MAC after the composite was soaked in phosphate-buffered solution for 12 weeks. The pH of the soaking medium varied from 6.9 to 7.5. MG-63 cells with an osteogenic phenotype were well adhered and spread on the composite surface. Viability and differentiation increased with time, indicating that the composite had no negative effects on MG-63 cells. Conclusion:The n-CDHA-MAC composite had good cytocompatibility and has potential to be used as a bone substitute.

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Section: Introductionmentioning

confidence: 67%

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

Hong

Gong²,

Yang³

et al. 2012

IJN

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“…11,12 The MAC that is composed of 6-aminocaproic acid and five amino acids exhibits good ductility and biocompatibility. 13 Our previous works have suggested that the amide-calcium-amide linkage and hydrogen bond formed between n-CDHA and MAC endow this biocomposite with eligible mechanical strength (compressive strength in the range of 88-129 MPa), degradability, cytocompatibility, and osteoconductivity. [10][11][12][13] However, the n-CDHA/MAC composite material has relatively poor flexibility, which limits its application in repairing complex and irregular bone defects.…”

Section: Introductionmentioning

confidence: 94%

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

Qi¹,

Hong²,

Qiao³

et al. 2013

IJN

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A novel injectable bone cement was developed by integration of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer (n-CDHA/MAC) and calcium sulfate hemihydrate (CSH; CaSO 4 · 1/2H 2 O). The structure, setting time, and compressive strength of the cement were investigated. The results showed that the cement with a liquid to powder ratio of 0.8 mL/g exhibited good injectability and appropriate setting time and mechanical properties. In vitro cell studies indicated that MC3T3-E1 cells cultured on the n-CDHA/MAC/CSH composite spread well and showed a good proliferation state. The alkaline phosphatase activity of the MC3T3-E1 cells cultured on the n-CDHA/MAC/CSH composite was significantly higher than that of the cells on pure CSH at 4 and 7 days of culture. The n-CDHA/MAC/CSH cement was implanted into critical size defects of the femoral condyle in rabbits to evaluate its biocompatibility and osteogenesis in vivo. Radiological and histological results indicated that introduction of the n-CDHA/MAC into CSH enhanced new bone formation, and the n-CDHA/MAC/CSH cement exhibited good biocompatibility and degradability. In conclusion, the injectable n-CDHA/MAC/CSH composite cement has a significant clinical advantage over pure CSH cement, and may be a promising bone graft substitute for the treatment of bone defects.

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“…24,25 The PAA copolymer could be degraded in HCL-Tris solution with weight loss of ~30 wt% after 12 weeks of soaking, and it had no significant effect on the pH value of the ambient environment during the degradation period. 26 If the pH value in the ambient solution was decreased, it was believed to induce the inflammatory reaction in vivo. 27 Cell culture experiments and in vivo implantation results showed that the PAA also had good biocompatibility.…”

mentioning

confidence: 99%

“…27 Cell culture experiments and in vivo implantation results showed that the PAA also had good biocompatibility. 26 When implanted in cortical bone of the dogs, the PAA copolymer implants were directly connected with the host bone tissue without obvious intervening connective layer, and some new bone tissues were found to extend along the copolymer surface, which was known as bone-bonding. 26 Bone-bonding could ensure that the implant integrated with natural bone through biochemical reaction at the interface between biomaterials and bone tissue, which was in favor of implant fixation in host bone.…”

mentioning

confidence: 99%

“…26 When implanted in cortical bone of the dogs, the PAA copolymer implants were directly connected with the host bone tissue without obvious intervening connective layer, and some new bone tissues were found to extend along the copolymer surface, which was known as bone-bonding. 26 Bone-bonding could ensure that the implant integrated with natural bone through biochemical reaction at the interface between biomaterials and bone tissue, which was in favor of implant fixation in host bone. 28 Therefore, these previous experiments suggested that the combination of PAA with n-CDHA would not have apparent adverse reaction with the bone tissue and surrounding biological macromolecules from tissue and blood, and it may have excellent biocompatibility and osteoconductivity as potential implants in orthopedic surgery.…”

mentioning

confidence: 99%

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In vivo biocompatibility of new nano-calcium-deficient hydroxyapatite/poly-amino acid complex biomaterials

Dai

et al. 2015

IJN

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Objective To evaluate the compatibility of novel nano-calcium-deficient hydroxyapatite/poly-amino acid (n-CDHA/PAA) complex biomaterials with muscle and bone tissue in an in vivo model. Methods Thirty-two New Zealand white rabbits were used in this study. Biomaterials were surgically implanted into each rabbit in the back erector spinae and in tibia with induced defect. Polyethylene was implanted into rabbits in the control group and n-CDHA/PAA into those of the experimental group. Animals were examined at four different points in time: 2 weeks, 4 weeks, 12 weeks, and 24 weeks after surgery. They were euthanized after embolization. Back erector spinae muscles with the surgical implants were examined after hematoxylin and eosin (HE) staining at these points in time. Tibia bones with the surgical implants were examined by X-ray and scanning electron microscopy (SEM) at these points in time to evaluate the interface of the bone with the implanted biomaterials. Bone tissues were sectioned and subjected to HE, Masson, and toluidine blue staining. Results HE staining of back erector spinae muscles at 4 weeks, 12 weeks, and 24 weeks after implantation of either n-CDHA/PAA or polyethylene showed disappearance of inflammation and normal arrangement in the peripheral tissue of implant biomaterials; no abnormal staining was observed. At 2 weeks after implantation, X-ray imaging of bone tissue samples in both experimental and control groups showed that the peripheral tissues of the implanted biomaterials were continuous and lacked bone osteolysis, absorption, necrosis, or osteomyelitis. The connection between implanted biomaterials and bone tissue was tight. The results of HE, Masson, toluidine blue staining and SEM confirmed that the implanted biomaterials were closely connected to the bone defect and that no rejection had taken place. The n-CDHA/PAA biomaterials induced differentiation of a large number of chondrocytes. New bone trabecula began to form at 4 weeks after implanting n-CDHA/PAA biomaterials, and lamellar bone gradually formed at 12 weeks and 24 weeks after implantation. Routine blood and kidney function tests showed no significant changes at 2 weeks and 24 weeks after implantation of both biomaterials. Conclusion n-CDHA/PAA composites showed good compatibility in in vivo model. In this study, n-CDHA/PAA were found to be safe, nontoxic, and biologically active in bone repair.

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Bone substitute biomedical material of multi-(amino acid) copolymer: in vitro degradation and biocompatibility

Cited by 32 publications

References 25 publications

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

Development and characterization of an injectable cement of nano calcium-deficient hydroxyapatite/multi(amino acid) copolymer/calcium sulfate hemihydrate for bone repair

In vivo biocompatibility of new nano-calcium-deficient hydroxyapatite/poly-amino acid complex biomaterials

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