Bone regeneration with BMP-2 delivered from keratose scaffolds

Guzman, Roche C. de; Saul, Justin M.; Ellenburg, Mary D.; Merrill, Michelle R.; Coan, H.B.; Smith, Thomas L.; Dyke, Mark Van

doi:10.1016/j.biomaterials.2012.11.002

Cited by 135 publications

(104 citation statements)

References 37 publications

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“…Xiao et al 23 used BMP-2 loaded hollow hydroxyapatite microspheres coated with PLGA to repair calvarial defects of rats, inducing significantly enhanced bone regeneration. Similar findings were revealed in studies on rats, [36][37] mice, 24 and sheep. 25 Our quantitative measurements on bone growth and the push-out testing results indicate that a combination of measurement methods may provide a way to standardize bone growth measurements.…”

Section: Discussionsupporting

confidence: 89%

Controlled‐release of bone morphogenetic protein‐2 from a microsphere coating applied to acid‐etched Ti6AL4V implants increases biological bone growth in vivo

Zhang

Sun

et al. 2014

Journal Orthopaedic Research

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A central clinical challenge regarding the surgical treatment of bone and joint conditions is the eventual loosening of an orthopedic implant as a result of insufficient bone ingrowth at the bone-implant interface. We investigated the in vivo effectiveness of a coating containing recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded microspheres applied to acid-etched Ti6Al4V cylinders for implantation. Three groups of rabbits (24 per group) were used for implantation: (1) acid-etched Ti6Al4V implants coated with a mixture of rhBMP-2-loaded microspheres (125 ng rhBMP-2/mg microspheres) and a-butyl cyanoacrylate; (2) acid-etched, uncoated implants; and (3) bare, smooth uncoated implants. After implantation, 12 rabbits from each group were used for bone ingrowth determination at 4, 5, 6, 7, 8, and 12 weeks (2 rabbits per time point), while the remainder were used for histological analysis and push-out testing at 12 weeks. Scanning electron microscopy showed significant improvement in bone growth of the rhBMP-2 microspheres/a-butyl cyanoacrylate group compared with the other groups (p < 0.01). Histological analysis and push-out testing also demonstrated enhanced bone growth of the rhBMP-2 group over that in the other two groups (p < 0.01). The rhBMP-2 group showed the most significant bone growth, suggesting that coating acid-etched implants with a mixture of rhBMP-2-loaded microspheres and a-butyl cyanoacrylate may be an effective method to improve the osseointegration of orthopedic implants. Keywords: rhBMP-2; bone growth; microsphere; Ti6Al4V; a-butyl cyanoacrylateThe alloy (titanium containing 6% aluminum and 4% vanadium) is used for orthopedic implants because of its high biocompatibility, corrosion resistance, easy formability, and increased strength compared to other titanium alloys.1 However, implants including those made of Ti6Al4V tend to produce wear particles that stimulate periprosthetic osteolysis. Wear particles are thought to promote osteolysis because they induce macrophages that then phagocytose the particles. 2-5Ti6Al4V implants with acid-etched surface offer a better surface for osteogenic cells to attach than bare Ti surfaces, thereby osteoconductivity. 6 In addition, acid-etched implants could stimulate vascular formation, which would support and promote bone ingrowth.7-10 Cortical implants with a pore size of 50-400 mm promote cortical bone ingrowth with relatively high fixation strength. 11Another strategy for promoting bone growth into implants involves integrating cytokine growth factors, such as bone morphogenetic proteins (BMPs) into the implant. BMPs are a group of cytokines that promote bone and cartilage formation. Recombinant human BMP-2 (rhBMP-2) has been purified, 12,13 and shown to enhance in vivo bone formation.14,15 BMP has been used for decades in treating spinal fusion, 16-18 dental implantation, 19 and implant osseointegration 20-21 with excellent results. McClellan et al. 22 reviewed cases where high-dose rhBMP-2 was used in the fusion of lumbar discs dur...

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Section: Discussionsupporting

confidence: 89%

Controlled‐release of bone morphogenetic protein‐2 from a microsphere coating applied to acid‐etched Ti6AL4V implants increases biological bone growth in vivo

Zhang

Sun

et al. 2014

Journal Orthopaedic Research

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“…Besides, keratin also possesses cell-binding motifs such as Leucine-Aspartic acidValine (LDV), which can potentially facilitate cell-matrix interactions [11]. Keratin was also reported recently to be a suitable vehicle for delivering BMP-2 for bone regeneration [12]. It was therefore hypothesized that the presence of crosslinked keratin could aid in improving the mechanical strength of CaP-coated PCL scaffolds, which would otherwise be compromised after coating with CaP.…”

Section: Introductionmentioning

confidence: 98%

Calcium phosphate coated Keratin–PCL scaffolds for potential bone tissue regeneration

Zhao

Lui

Choo

et al. 2015

Materials Science and Engineering: C

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“…Once damage occurs, hematoma will appear around the site of trauma triggering fibrous tissues to form a lesion area and to develop into callus. 32 In some ways, the bone will activate all intraosseous and periosteum osteoblasts maximally in the defect areas. Number of new osteoblasts are formed from progenitor cells as bone stem cells in the surface of the tissue covering the bones, called as bone membrane.…”

Section: Discussionmentioning

confidence: 99%

Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process

Sari

Hermanto

Divilia

et al. 2016

Dent. J. (Majalah Kedokteran Gigi)

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Bone regeneration with BMP-2 delivered from keratose scaffolds

Cited by 135 publications

References 37 publications

Controlled‐release of bone morphogenetic protein‐2 from a microsphere coating applied to acid‐etched Ti6AL4V implants increases biological bone growth in vivo

Controlled‐release of bone morphogenetic protein‐2 from a microsphere coating applied to acid‐etched Ti6AL4V implants increases biological bone growth in vivo

Calcium phosphate coated Keratin–PCL scaffolds for potential bone tissue regeneration

Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process

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