Bone marrow and peripheral white blood cells number is affected by sleep deprivation in a murine experimental model

Abstract

Sleep deficit and related disorders are becoming increasingly prevalent in modern life and an extensive literature has documented that acute or chronic sleep deprivation can lead to several physiological consequences. Here, we evaluated the effects of sleep deprivation on hematopoietic composition of either bone marrow or peripheral blood. Mice were subjected to paradoxical sleep deprivation (PSD) for 72 h by modified multiple platform method, with or without an additional sleep recovery (SR) period of 10 days. PSD decreased total cellularity of the bone marrow and peripheral blood concomitantly. Subsequent analysis of cell composition showed that absolute number of hematopoietic stem/progenitor cells and colony-forming units was decreased. Moreover, the absolute number of granulocytes and monocytes in bone marrow was reduced in PSD group. These alterations were paralleled by an accumulation of neutrophils and monocytes in peripheral blood. PSD also induced lymphopenia in the circulation. To the best of our knowledge, this is the first study that demonstrates the importance of sleep on the hematopoietic microenvironment and provides new insights into the relationship between sleep and the immune system.

Effects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis

“…F) confirms the SD‐induced alterations in medullary B lymphopoiesis verified by flow cytometry analysis, based on the diminishing percentage of both lymphoid progenitors and mature B lymphocytes. This finding is in line to the observed effect of SD on lymphocyte clonogenicity (Guariniello et al, ).…”

“…Several studies in the field of sleep have shown the importance of this behavioral state for the homeostasis of the immune system. SD can result in many losses concerning the bidirectional communication of the immune system with the nervous and endocrine systems (Lange et al, , ; Motivala, ; Reis et al, ), reduction in the weight of the lymphoid organs (Zager et al, ) and disruption of the homeostasis of immune cells (Zager et al, ; Guariniello et al, ; Lungato et al, ; Maragno‐Correa et al, ). In this study, it was found that the total populations of B lymphocytes in the spleen tissue were reduced after 72 h of SD (Fig.…”

Sleep Disruption in Hematopoietic Cell Transplantation Recipients: Prevalence, Severity, and Clinical Management

“…For example, previous research in patients undergoing standard-dose chemotherapy suggests that sleep disruption occurs first in a cascade of symptoms, contributing to increases in fatigue and in turn, depression (71). In addition, preliminary research suggests that sleep disruption may negatively impact immune response and reconstitution (72), although this has not been demonstrated in the context of HCT. Taken together, these studies argue for early intervention to manage sleep disruption and disorders in HCT recipients.…”

Serum Amyloid A Production Is Triggered by Sleep Deprivation in Mice and Humans: Is That the Link between Sleep Loss and Associated Comorbidities?

“…For PSD experiments, the animals were randomly assigned into three groups: the control group, the PSD group and the PSR group. PSD animals were sleep deprived for 72 consecutive hours, using the multiple platform method as previously described [3,13,14,15]. The PSR mice were sleep deprived for 72 consecutive hours followed by 24 h in sleep rebound period.…”