Bone and cartilage metabolism markers in synovial fluid of the hip joint with secondary osteoarthritis

Yamaguchi, Ryosuke; Yamamoto, Takuya; Motomura, Goro; Ikemura, Satoshi; Iwasaki, Kenyu; Zhao, Garida; Doi, Takehiko; Iwamoto, Yukihide

doi:10.1093/rheumatology/keu253

Cited by 24 publications

(21 citation statements)

References 15 publications

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“…[19, 34] Our results support these findings that at least mononucleated TRAcP positive cells can be found in synovial tissue, as can be seen in Fig 3. This suggests that TRAcP is produced in the synovial tissue.…”

Section: Discussionsupporting

confidence: 89%

Increased amount of phosphorylated proinflammatory osteopontin in rheumatoid arthritis synovia is associated to decreased tartrate-resistant acid phosphatase 5B/5A ratio

et al. 2017

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BackgroundOsteopontin (OPN) is an immunoregulatory protein which production increases in both rheumatoid arthritis (RA) and osteoarthritis (OA). Phosphorylated osteopontin (Phospho-OPN) is known to increase macrophage and osteoclast activation, this process is controlled by extracellular tartrate-resistant acid phosphatase (TRAcP), also a biomarker for RA. Here, we evaluated the phosphorylation status of OPN in RA and OA synovia, as well as its correlation with TRAcP isoforms.MethodsSynovial tissue and fluid were obtained from 24 RA (14 seropositive and 10 seronegative) and 24 OA patients. Western blotting was used to analyze the extent of OPN phosphorylation. TRAcP isoforms were measured in synovial fluid using ELISA; immunohistochemistry assessed the distribution of OPN and TRAcP expressing cells in the synovial tissue, especially distinguishing between the TRAcP isoforms.ResultsFull-length OPN was more phosphorylated in RA than in OA (p<0.05). The thrombin cleaved C-terminal end of OPN was also more phosphorylated in RA (p<0.05). RA patients had a lower concentration of TRAcP 5B and higher concentration of less active 5A in their synovial fluid compared to OA patients. The TRAcP 5B/5A ratio was decreased in RA and correlated negatively with the amount of phospho-OPN (p<0.05). TRAcP positive cells for both isoforms were found all along the synovial lining; OPN antibody staining was localized in the extracellular matrix.ConclusionOur data suggests that in RA the synovial fluid contains insufficient amounts of TRAcP 5B which increase levels of the proinflammatory phospho-OPN. This may lead to increased macrophage and osteoclast activation, resulting in the increased local inflammation and bone resorption present in RA joints.

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Section: Discussionsupporting

confidence: 89%

Increased amount of phosphorylated proinflammatory osteopontin in rheumatoid arthritis synovia is associated to decreased tartrate-resistant acid phosphatase 5B/5A ratio

et al. 2017

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“…Control [1,5,12,18,19,49]; atrophic versus hypertrophic in two studies [13,14]; and radiographic OA classification in five studies [6,23,42,47,48]. Two studies [15,20] analyzed minimum joint space width as a continuous variable relative to biomarker levels.…”

Section: Literature Searchmentioning

confidence: 99%

What Is the Utility of Biomarkers for Assessing the Pathophysiology of Hip Osteoarthritis? A Systematic Review

Nepple

Thomason

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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“…6). In the joint fluid, a high level of tartrate-resistant acid phosphatase 5b (4190 mU/ dl) was confirmed, indicating high bone resorption within the hip joint [6]. These findings are characteristic of RDA [6][7][8].…”

Section: Introductionmentioning

confidence: 72%

Rapidly destructive arthrosis of the hip joint in a young adult with systemic lupus erythematosus

et al. 2015

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A 37-year-old female had been treated with corticosteroids for systemic lupus erythematosus clinically diagnosed at age 10. She suddenly had right hip pain without any antecedent trauma. Four months after the onset of pain, she visited her primary care physician. On magnetic resonance imaging, joint space narrowing at the weight-bearing area was already seen with bone marrow edematous lesions in both the femoral head and acetabulum. She was treated non-operatively; however, her pain continued to worsen in severity. Thirteen months after the onset of pain, she was referred to our hospital. A plain radiograph showed subluxation of the collapsed femoral head accompanied by destruction of the acetabular rim. Because of her severe intractable pain, she underwent total hip arthroplasty 1 month after her first visit. Histological examination of the resected femoral head revealed pseudogranulomatous lesions along with prominent callus formation, suggesting rapid destruction of the femoral head.

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Bone and cartilage metabolism markers in synovial fluid of the hip joint with secondary osteoarthritis

Abstract: The ONFH patients showed a relatively bone formative condition before the osteoarthritic stage and maintained a higher rate of cartilage turnover throughout several stages compared with the RDA and DDH patients. RDA patients were characterized by a significantly high osteoclast activity.

Cited by 24 publications

References 15 publications

Increased amount of phosphorylated proinflammatory osteopontin in rheumatoid arthritis synovia is associated to decreased tartrate-resistant acid phosphatase 5B/5A ratio

Increased amount of phosphorylated proinflammatory osteopontin in rheumatoid arthritis synovia is associated to decreased tartrate-resistant acid phosphatase 5B/5A ratio

What Is the Utility of Biomarkers for Assessing the Pathophysiology of Hip Osteoarthritis? A Systematic Review

Rapidly destructive arthrosis of the hip joint in a young adult with systemic lupus erythematosus

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