Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish

Lovely, C. Ben; Swartz, Mary E.; McCarthy, Neil; Norrie, Jacqueline L.; Eberhart, Johann K.

doi:10.1242/dev.129379

Cited by 24 publications

(56 citation statements)

References 66 publications

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“…During pouch segmentation, foxa1 is expressed not only in the medial pharyngeal endoderm but also in the first pouch [34]. Consistent with previous reports [40], the expression of foxa1 was not notably changed in the medial pharyngeal endoderm, but obviously decreased in the first pouch at 26 hpf in embryos treated with 10 μM Dorsomorphin (Fig 4A). Meanwhile, blocking BMP signaling using 10 μM Dorsomorphin also led to decreased expression of nkx2 .…”

Section: Resultssupporting

confidence: 90%

“…BMP signaling plays important roles in the induction and anteroposterior patterning of the endoderm during gastrulation [38,39]. However, past work has shown that blocking BMP activity at the early somite stages has no effect on early endoderm genesis [40]. To this end, DMH1-treated Tg(sox17 : GFP) transgenic embryos stably expressed GFP fluorescence in unaltered expression domains at 6- and 10-somite stages when compared with control embryos, suggesting that the pan-endoderm was properly developed (S6 Fig).…”

Section: Resultsmentioning

confidence: 99%

“…However, a previous study showed that while BMP signaling was crucial for endodermal pouch morphogenesis, it was not necessary for lateral pharyngeal endoderm identity [40]. In this work, a lower dose (10 μM) of Dorsomorphin was used for BMP signal inhibition immediately following gastrulation, which resulted in morphological pouch defects and a reduced expression of the pouch epithelium marker pdgfαb , while no obvious effect on pdgfαa expression [40]. Given this, we speculated that BMP signaling in the lateral pharyngeal endoderm might have a dose-dependent effect.…”

Section: Resultsmentioning

confidence: 99%

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BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish

Guan

Yang

et al. 2019

PLoS Genet

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Pharyngeal pouches, a series of outpocketings that bud from the foregut endoderm, are essential to the formation of craniofacial skeleton as well as several important structures like parathyroid and thymus. However, whether pharyngeal pouch progenitors exist in the developing gut tube remains unknown. Here, taking advantage of cell lineage tracing and transgenic ablation technologies, we identified a population of nkx2 . 3 + pouch progenitors in zebrafish embryos and demonstrated an essential requirement of ectodermal BMP2b for their specification. At early somite stages, nkx2 . 3 + cells located at lateral region of pharyngeal endoderm give rise to the pouch epithelium except a subpopulation expressing pdgfαa rather than nkx2 . 3 . A small-scale screen of chemical inhibitors reveals that BMP signaling is necessary to specify these progenitors. Loss-of-function analyses show that BMP2b, expressed in the pharyngeal ectoderm, actives Smad effectors in endodermal cells to induce nkx2 . 3 + progenitors. Collectively, our study provides in vivo evidence for the existence of pouch progenitors and highlights the importance of BMP2b signaling in progenitor specification.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish

Guan

Yang

et al. 2019

PLoS Genet

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“…Fstl1 has a domain similar to follistatin. In addition, its orthologue in zebrafish, zfstl 1/2, functions analogously to nog1 and chd to antagonize BMP activity during zebrafish development [37-39]. Previous reports show that Fstl1 can bind to BMP4 with a K d similar to that of follistatin binding to activin [27, 32, 40, 41].…”

Section: Discussionmentioning

confidence: 99%

Fstl1 Promotes Glioma Growth Through the BMP4/Smad1/5/8 Signaling Pathway

Jin¹,

Nie²,

Zhou³

et al. 2017

Cell Physiol Biochem

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Background: Gliomas result in the highest morbidity and mortality rates of intracranial primary central nervous system tumors because of their aggressive growth characteristics and high postoperative recurrence. They are characterized by genetic instability, intratumoral histopathological variability and unpredictable clinical behavior in patients. Proliferation is a key aspect of the clinical progression of malignant gliomas, complicating complete surgical resection and enabling tumor regrowth and further proliferation of the surviving tumor cells. Methods: The expression of Fstl1 was detected by western blotting and qRT-PCR. We used cell proliferation and colony formation assays to measure proliferation. Then, flow cytometry was used to analyze cell cycle progression. The expression of Fstl1, p-Smad1/5/8 and p21 in GBM tissue sections was evaluated using immunohistochemical staining. Furthermore, we used coimmunoprecipitation (Co-IP) and immunoprecipitation to validate the relationship between Fstl1, BMP4 and BMPR2. Finally, we used orthotopic xenograft studies to measure the growth of tumors in vivo. Results: We found that follistatin-like 1 (Fstl1) was upregulated in high-grade glioma specimens and that its levels correlated with poor prognosis. Fstl1 upregulation increased cell proliferation, colony formation and cell cycle progression, while its knockdown inhibited these processes. Moreover, Fstl1 interacted with bone morphogenetic protein (BMP) 4, but not BMP receptor (BMPR) 2, and competitively inhibited their association. Furthermore, Fstl1 overexpression suppressed the activation of the BMP4/Smad1/5/8 signaling pathway, while BMP4 overexpression reversed this effect. Conclusion: Our study demonstrated that Fstl1 promoted glioma growth through the BMP4/Smad1/5/8 signaling pathway, and these findings suggest potential new glioblastoma treatment strategies.

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“…Furthermore, a recent study indicated that endodermal BMP signaling promotes FGF signaling to control pharyngeal pouch development during early somitogenesis (Lovely et al, 2016), suggesting these signals may be expressed at an appropriate developmental time to function downstream of Wnt signaling in patterning PM progenitor cells. However, our analysis of BMP and FGF ligands within zebrafish embryos after Wnt manipulation only revealed patterning defects that are consistent with early axis specification defects without effects on the pharyngeal pouches (Supplementary Material – Figure S6 and Figure S7).…”

Section: Discussionmentioning

confidence: 99%

Wnt signaling balances specification of the cardiac and pharyngeal muscle fields

Mandal

Holowiecki

Song

et al. 2017

Mechanisms of Development

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Canonical Wnt/β-catenin (Wnt) signaling plays multiple conserved roles during fate specification of cardiac progenitors in developing vertebrate embryos. Although lineage analysis in ascidians and mice has indicated there is a close relationship between the cardiac second heart field (SHF) and pharyngeal muscle (PM) progenitors, the signals underlying directional fate decisions of the cells within the cardio-pharyngeal muscle field in vertebrates are not yet understood. Here, we examined the temporal requirements of Wnt signaling in cardiac and PM development. In contrast to a previous report in chicken embryos that suggested Wnt inhibits PM development during somitogenesis, we find that in zebrafish embryos Wnt signaling is sufficient to repress PM development during anterior-posterior patterning. Importantly, the temporal sensitivity of dorso-anterior PMs to increased Wnt signaling largely overlaps with when Wnt signaling promotes specification of the adjacent cardiac progenitors. Furthermore, we find that excess early Wnt signaling can cell autonomously promote expansion of the first heart field (FHF) progenitors at the expense of PM and SHF within the anterior lateral plate mesoderm (ALPM). Our study provides insight into an antagonistic developmental mechanism that balances the sizes of the adjacent cardiac and PM progenitor fields in early vertebrate embryos.

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Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish

Cited by 24 publications

References 66 publications

BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish

BMP signaling is required for nkx2.3-positive pharyngeal pouch progenitor specification in zebrafish

Fstl1 Promotes Glioma Growth Through the BMP4/Smad1/5/8 Signaling Pathway

Wnt signaling balances specification of the cardiac and pharyngeal muscle fields

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