Blutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in mice

Kassuya, Roberto Mikio; Radai, Joyce Alencar Santos; Macorini, Luís Fernando Benitez; Nunes, Viktor Krejci; Salvador, Marcos José; Leite, Patrícia Regina Terço; Oliveira, Rodrigo Juliano; Croda, Júlio; Arena, Arielle Cristina; Kassuya, Cândida Aparecida Leite

doi:10.1007/s10787-020-00752-0

Cited by 9 publications

(5 citation statements)

References 42 publications

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“…In BCG pleurisy model, oral doses of 30 and 100 mg/kg of EESM did not interfere with leukocyte migration to the pleural exudate however it induced a signi cant inhibition on the interleukin-1 beta (IL-1β) levels in serum and pleural exudate. The increase in IL-1β after 7 days in BCG model may be correlated with the mycobacterial as showed previously by our group [26] . [35] indicated that mice cient in interleukin (IL) showed reduction bacterial load 35 days' post infection.…”

Section: Discussionsupporting

confidence: 82%

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Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

Leitão¹,

Radai²,

Macorini³

et al. 2022

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Objectives: This study investigated the antimycobacterial, anti-in ammatory and antihyperalgesic effects of hydroalcoholic extract from leaves of S. marginata (EESM) in in vitro and in vivo models.Methods and Results: EESM (0.98-1000 µg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced in ammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw in ammation and M. bovis (bacillus Calmette-Guerin -BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 µg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 µg/mL while EESM did not interfere against P. aeruginosa growth. EESM signi cantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw in ammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCG-induced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM signi cantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA.Conclusion: This study con rms the EESM anti-in ammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.

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Section: Discussionsupporting

confidence: 82%

“…The spleen and liver were macerated with 1 mL of sterile saline solution, and 0.1 mL of the suspension was plated on 7H11 agar. The cells were cultured for 60 days at 37°C in 5% CO 2 [26] .…”

Section: Leukocyte Migration Il-1β Dosage and Cfu Growth In Bcg-induc...mentioning

confidence: 99%

Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

Leitão¹,

Radai²,

Macorini³

et al. 2022

Self Cite

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show abstract

“…In BCG pleurisy model, oral doses of 30 and 100 mg/kg of EESM did not interfere with leukocyte migration to the pleural exudate however it induced a signi cant inhibition on the interleukin-1 beta (IL-1β) levels in serum and pleural exudate. The increase in IL-1β levels after 7 days in BCG model may be correlated with the mycobacterial growth, as showed previously by our group (Kassuya et al, 2020). Bourigault et al (2013) indicated that mice de cient in interleukin (IL) showed reduction in bacterial load 35 days' post infection.…”

Section: Discussionsupporting

confidence: 71%

“…The spleen and liver were macerated with 1 mL of sterile saline solution, and 0.1 mL of the suspension was plated on 7H11 agar. The cells were cultured for 60 days at 37°C in 5% CO 2 (Kassuya et al, 2020).…”

Section: Leukocyte Migration Il-1β Dosage and Cfu Growth In Bcg-indumentioning

confidence: 99%

Serjania marginata Casar. hydroalcoholic extract reduced cytokine and inflammatory parameters in experimental models of inflammation and infection in mice

Leitão¹,

Radai²,

Macorini³

et al. 2021

Preprint

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This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of EESM in in vitro and in vivo models. EESM (0.98–1000 µg/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 µg/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 µg/mL while EESM did not interfere with against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCG-induced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1β in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.

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“…Previous biological studies revealed leishmanicidal, antimicrobial, and anti-inflammatory effects of different preparations of B. portulacoides 7 , 8 . However, the available literature does not report the effects of the species on the renal and cardiovascular systems.…”

Section: Introductionmentioning

confidence: 99%

The NO-cGMP-K+ Channel Pathway Participates in Diuretic and Cardioprotective Effects of Blutaparon portulacoides in Spontaneously Hypertensive Rats

et al. 2022

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Blutaparon portulacoides is a Brazilian plant species that is widely used in folk medicine. The present study investigated the role of an aqueous extract of B. portulacoides against hypertension in spontaneously hypertensive rats. The aqueous extract of B. portulacoides was obtained from the whole plant. Its chemical profile was analyzed by ultraperformance liquid chromatography-tandem mass spectrometry. The acute toxicity of the aqueous extract of B. portulacoides was evaluated in female Wistar rats. Male 6-month-old spontaneously hypertensive rats then received the aqueous extract of B. portulacoides (30, 100, and 300 mg/kg), hydrochlorothiazide (25 mg/kg), or vehicle once daily for 28 days. On days 1, 14, and 28, the diuretic effects of the aqueous extract of B. portulacoides were evaluated. The role of prostaglandins and the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway in the diuretic activity of the aqueous extract of B. portulacoides was also investigated. At the end of the treatment, hepatic and renal biochemical markers, serum nitrotyrosine, malondialdehyde, nitrite, and aldosterone levels, and angiotensin-converting enzyme activity were measured. The electrocardiographic profile, blood pressure, and renal vascular reactivity were also assessed. The heart, kidneys, and liver were collected to determine relative organ weight, histopathology, and cardiac morphometry. Caffeic acid, ferulic acid, and several flavonoids were identified in the aqueous extract of B. portulacoides. No signs of toxicity were observed. Prolonged treatment with the aqueous extract of B. portulacoides (300 mg/kg) induced significant diuretic activity by activating the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway. These effects reduced blood pressure and oxidative stress and prevented renal vascular dysfunction and left ventricular hypertrophy that was induced by hypertension. Overall, the present data suggest that the aqueous extract of B. portulacoides has important diuretic and cardioprotective effects by activation of the nitric oxide-cyclic guanosine monophosphate-potassium channel pathway.

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Blutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in mice

Cited by 9 publications

References 42 publications

Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

Serjania marginata Casar. hydroalcoholic extract reduced cytokine and inflammatory parameters in experimental models of inflammation and infection in mice

The NO-cGMP-K+ Channel Pathway Participates in Diuretic and Cardioprotective Effects of Blutaparon portulacoides in Spontaneously Hypertensive Rats

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