Blue‐stain fungi isolated from freshly felled Scots pine logs in Poland, including <i>Leptographium sosnaicola</i> sp. nov

Jankowiak, Robert; Szewczyk, Grzegorz; Bilański, Piotr; Jazłowiecka, Dominika; Harabin, None Beata; Linnakoski, Riikka

doi:10.1111/efp.12672

Cited by 14 publications

(18 citation statements)

References 57 publications

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“…The authors showed that L. sosnaicola formed a monophyletic line within other members of Leptographium , and its morphology did not resemble any known Leptographium species. Our results also show L. sosnaicola L2.6 within L. lundburgii complex and totally separated from the other sister species, corroborating the previous observations (Jankowiak et al, 2021 ). The other Leptographium species identified L. terebrantis , firstly described as associated with the black turpentine beetle Dendroctonus terebrans (Barras and Perry, 1971 ), was reported as pathogen of woody roots and recently related to crown symptoms and tree mortality in P. taeda (Mensah et al, 2021 ).…”

Section: Discussionsupporting

confidence: 93%

“…As suggested by Wang et al ( 2018 ), the dominance of O. ips can have either an indigenous origin or be an effective adaptation to local pine forests. In fact, the presence of O. ips in non-infected trees in Seia could be explained by natural infection with various species of bark beetles as is common in European pine forests (Chang et al, 2017 ; Jankowiak et al, 2021 ). The other ophiostomatoid isolates were Leptographium sp., Graphilbum sp., and Sporothrix sp., which were also described in other studies (Zhao et al, 2013 , 2018 ; Wang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…For the first time, we report Leptographium species associated with PWN-infected P. pinaster trees in Portugal, namely L. sosnaicola and L. terebrantis . Leptographium sosnaicola was described in logs of P. sylvestris in Poland and probably vectored by Ips sexdentatus (Jankowiak et al, 2021 ). The authors showed that L. sosnaicola formed a monophyletic line within other members of Leptographium , and its morphology did not resemble any known Leptographium species.…”

Section: Discussionmentioning

confidence: 99%

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Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus

Vicente

Soares²,

Faria³

et al. 2022

Front. Plant Sci.

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Considered one of the most devastating plant–parasitic nematodes worldwide, Bursaphelenchus xylophilus (commonly known as pinewood nematode, PWN) is the causal agent of the pine wilt disease in the Eurasian coniferous forests. This migratory parasitic nematode is carried by an insect vector (Monochamus spp.) into the host tree (Pinus species), where it can feed on parenchymal cells and reproduce massively, resulting in the tree wilting. In declining trees, PWN populations are strongly dependent on fungal communities colonizing the host (predominantly ophiostomatoid fungi known to cause sapwood blue-staining, the blue-stain fungi), which not only influence their development and life cycle but also the number of individuals carried by the insect vector into a new host. Our main aim is to understand if PWN-associated mycobiota plays a key role in the development of PWD, in interaction with the PWN and the insect vector, and to what extent it can be targeted to disrupt the disease cycle. For this purpose, we characterized the fungal communities of Pinus pinaster trees infected and non-infected with PWN in three collection sites in Continental Portugal with different PWD temporal incidences. Our results showed that non-infected P. pinaster mycoflora is more diverse (in terms of abundance and fungal richness) than PWN-infected pine trees in the most recent PWD foci, as opposed to the fungal communities of long-term PWD history sites. Then, due to their ecological importance for PWN survival, representatives of the main ophiostomatoid fungi isolated (Ophiostoma, Leptographium, and Graphilbum) were characterized for their adaptative response to temperature, competition in-between taxa, and as food source for PWN. Under the conditions studied, Leptographium isolates showed promising results for PWN control. They could outcompete the other species, especially O. ips, and significantly reduce the development of PWN populations when compared to Botrytis cinerea (routinely used for PWN lab culturing), suggesting this to be a natural antagonist not only for the other blue-stain species but also for the PWN.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus

Vicente

Soares²,

Faria³

et al. 2022

Front. Plant Sci.

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“…While not all ophiostomatoid fungi are responsible for tree mortality, many are well recognized as the causal agents of blue stain (sap stain) in the wood of economically important tree hosts (Kirisits 2004;Seifert et al 2013). This is particularly true for pine (Pinus) plantations globally (Seifert et al 2013;de Errasti et al 2018;Jankowiak et al 2021). Systematic surveys of bark beetles and ophiostomatoid fungi associated with pine have been completed in North and Central America (Zhou et al 2004a;Kim et al 2011;Klepzig and Hofstetter 2011;Taerum et al 2013;Marincowitz et al 2020), Europe (Linnakoski et al 2012;Romón et al 2014;Jankowiak et al 2012Jankowiak et al , 2020, Asia (Zhou et al 2013;Masuya et al 2013;Kirisits et al 2013), with a significant number of surveys conducted recently in China (Chang et al 2017(Chang et al , 2019Wang et al 2018Wang et al , 2019Wang et al , 2020, South America (Zhou et al 2004b;de Errasti et al 2018) and New Zealand (Thwaites et al 2005(Thwaites et al , 2013.…”

Section: Introductionmentioning

confidence: 99%

Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov.

et al. 2021

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The ophiostomatoid fungi are an assemblage of ascomycetes which are arguably best-known for their associations with bark and ambrosia beetles (Curculonidae) and blue stain (sap stain) of many economically important tree species. These fungi are considered a significant threat to coniferous forests, which has resulted in numerous studies characterising the diversity of bark beetles and their ophiostomatoid associates globally. The diversity of ophiostomatoid fungi present in Australian pine plantations, however, remains largely undetermined. The aims of this study were therefore to reconsider the diversity of ophiostomatoid fungi associated with Pinus in Australia, and to establish the baseline of expected taxa found within these plantation ecosystems. To achieve this, we reviewed Australian plant pathogen reference collections, and analysed samples collected during forest health surveillance programs from the major pine growing regions in south-eastern Australia. In total, 135 ophiostomatoid isolates (15 from reference collections and 120 collected during the current study) were assessed using morphological identification and ITS screening which putatively distinguished 15 taxonomic groups. Whole genome sequencing (WGS) of representative isolates from each taxon was performed to obtain high-quality sequence data for multi-locus phylogenetic analysis. Our results revealed a greater than expected diversity, expanding the status of ophiostomatoid fungi associated with Pinus in Australia to include 14 species from six genera in the Ophiostomatales and a single species residing in the Microascales. While most of these were already known to science, our study includes seven first records for Australia and the description of one new species, Graphilbum ipis-grandicollis sp. nov.. This study also provides an early example of whole genome sequencing (WGS) approaches replacing traditional PCR-based methods for taxonomic surveys. This not only allowed for robust multi-locus sequence extraction during taxonomic assessment, but also permitted the rapid establishment of a curated genomic database for ophiostomatoid fungi which will continue to aid in the development of improved diagnostic resources and capabilities for Australian biosecurity.

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“…Description of these new species brings the total number of species in this genus to 62, of which 16 occur in Poland. These include the six species described here as well as S. aurorae (Jankowiak et al 2019b), S. cantabriensis (Jankowiak et al 2017), S. dentifunda (Aghayeva et al 2005, Jankowiak et al 2019b, S. eucastaneae (Jankowiak et al 2019a(Jankowiak et al , 2019b(Jankowiak et al , 2021, S. fusiformis (Jankowiak et al 2019a(Jankowiak et al , 2019b, S. inflata (Jankowiak et al 2012;Bilański 2013a, 2013b), S. inflata '2' (Jankowiak et al 2019a(Jankowiak et al , 2019b, S. prolifera (Kowalski and Butin 1989;Jankowiak et al 2019aJankowiak et al , 2019b, S. stenoceras, (Kowalski and Butin 1989;Bilański 2013b, Jankowiak et al 2019b) and S. variecibatus (Jankowiak and Bilański 2013b). All of the species described in this study are morphologically similar, having asexual states with hyaline or lightly pigmented conidia produced holoblastically on denticulate conidiogenous cells or directly from the hyphae.…”

Section: Discussionmentioning

confidence: 99%

Six new species of Sporothrix from hardwood trees in Poland

Ostafińska¹,

Jankowiak²,

Bilański³

et al. 2021

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Sporothrix (Sordariales, Ascomycota) is a well-supported monophyletic lineage within the Ophiostomatales, species of which occur in a diverse range of habitats including on forest trees, in the soil, associated with bark beetles and mites as well as on the fruiting bodies of some Basidiomycota. Several species have also been reported as important human and animal pathogens. During surveys of insect- and wound-associated Ophiostomatales from hardwood trees in Poland, many isolates with affinity to Sporothrix were recovered. In the present study, six undescribed Sporothrix spp. collected during these surveys are characterized based on their morphological characteristics and multi-locus phylogenenetic inference. They are described as Sporothrix cavum, Sporothrix cracoviensis, S. cryptarchum, S. fraxini, S. resoviensis, and S. undulata. Two of the Sporothrix spp. reside in the S. gossypina-complex, while one forms part of the S. stenoceras-complex. One Sporothrix sp. is a member of lineage F, and two other species grouped outside any of the currently defined species complexes. All the newly described species were recovered from hardwood habitats in association with sub-cortical insects, wounds or woodpecker cavities. These species were morphologically similar, with predominantly asexual states having hyaline or lightly pigmented conidia, which produce holoblastically on denticulate conidiogenous cells. Five of the new taxa produce ascomata with necks terminating in long ostiolar hyphae and allantoid ascospores without sheaths. The results suggest that Sporothrix species are common members of the Ophiostomatales in hardwood ecosystems of Poland.

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Blue‐stain fungi isolated from freshly felled Scots pine logs in Poland, including Leptographium sosnaicola sp. nov

Cited by 14 publications

References 57 publications

Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus

Fungal Communities of the Pine Wilt Disease Complex: Studying the Interaction of Ophiostomatales With Bursaphelenchus xylophilus

Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov.

Six new species of Sporothrix from hardwood trees in Poland

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