Blood oxidative stress in amyotrophic lateral sclerosis

Bonnefont‐Rousselot, Dominique; Lacomblez, Lucette; Jaudon, M C; Lepage, Sylvie; Salachas, François; Bensimon, Gilbert; Bizard, Catherine; Doppler, Valérie; Delattre, Jacques; Meininger, Vincent

doi:10.1016/s0022-510x(00)00365-8

Cited by 89 publications

(56 citation statements)

References 38 publications

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“…Several recent clinical studies support an oxidative stress hypothesis as a number of biomarkers for oxidative stress have been found abnormal in ALS [6][7][8][9][10][11]. In particular, Bogdanov et al.…”

Section: Introductionmentioning

confidence: 92%

Oxidative stress biomarkers in sporadic ALS

Mitsumoto

Santella

Liu

et al. 2008

Amyotrophic Lateral Sclerosis

142

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Objective-To investigate oxidative stress biomarkers in a cross-sectional pilot study of 50 participants with sporadic ALS (sALS) compared to 46 control subjects.Methods-We measured urinary 8-oxodeoxyguanosine (8-oxodG), urinary 15-F 2t -isoprostane (IsoP), and plasma protein carbonyl by ELISA methods. We also determined if ELISA measurement of 8-oxodG could be validated against measures from high pressure liquid chromatography coupled with electrochemical detection, the current standard method.Results-8-oxodG and IsoP levels adjusted for creatinine were significantly elevated in sALS participants. These differences persisted after age and gender were controlled in regression analyses. These markers are highly and positively correlated with each other. 8-oxodG measured by the two techniques from the same urine sample were positively correlated (P < .0001). Protein carbonyl was not different between sALS participants and controls.Conclusion-Using ELISA we confirmed that certain oxidative stress biomarkers were elevated in sALS participants. ELISA may be reliable and thus useful in epidemiology studies requiring

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Section: Introductionmentioning

confidence: 92%

Oxidative stress biomarkers in sporadic ALS

Mitsumoto

Santella

Liu

et al. 2008

Amyotrophic Lateral Sclerosis

142

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“…The effects of riluzole in ALS could also be partially explained by increased glucose influx in damaged neurons and skeletal muscle tissues [145], and the drug might act as an antioxidant [143,146,147], offsetting the usual negative regulation of glutamate uptake in response to oxidative stress [148][149][150]. Lastly, riluzole could inhibit GABAergic transmission and potentiate function of postsynaptic GABA (A)-receptors [151].…”

Section: +mentioning

confidence: 99%

The Glutamate Hypothesis in ALS: Pathophysiology and Drug Development

Blasco¹,

Mavel²,

Corcia³

et al. 2014

CMC

131

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“…Oxidative damage to spinal cord proteins [1][2][3] , presence of 4-hydroxynonenal, a lipid peroxidation product, in CSF [4] and increased 3-nitrotyrosine (NT) in CSF [5] were observed in ALS patients, even if not all previous studies are in agreement [6] . Moreover, significantly higher levels of 4-hydroxynonenal in serum [7] , increased levels of free 8-hydroxydeoxyguanosine (a marker of DNA damage) in urine, plasma and CSF [8] , high levels of blood free radicals [9] , thiobarbituric-acid-reactive species, protein-associated carbonyls [10,11] and increased total protein oxidation in lymphocytes [12] have also been reported in ALS patients.…”

Section: Introductionmentioning

confidence: 99%

Lack of Evidence for Oxidative Stress in Sporadic Amyotrophic Lateral Sclerosis Fibroblasts

et al. 2008

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Background: It is conceivable that an early therapeutic intervention in amyotrophic lateral sclerosis (ALS) would lead to better results in terms of disease progression for these patients. One possible strategy to increase the sensitivity of the diagnosis is represented by the use of biological parameters reflecting, for example, oxidative stress alterations associated with ALS. Such biomarkers would be valuable tools both for a better diagnostic evaluation and for studying the impact of therapeutic interventions on the disease course. A special category of experimental models is represented by peripheral cells obtained directly from patients (ex vivo). Objective: In this study, primary fibroblasts obtained from 10 sporadic ALS (SALS) patients and 10 healthy matched controls were used to investigate a panel of parameters related to the oxidative status. Methods: Reactive oxygen species production, protein carbonylation and nitration, susceptibility to hydrogen peroxide exposure, p38-mitogen-activated protein kinase activation and adenosine triphosphate intracellular content were evaluated. Results: No significant difference was observed in all investigated parameters between patient and control cells, and no correlation with the disease severity was found. Conclusion: Collectively, our data show no major alterations of the oxidative and bioenergetic status in SALS cultured fibroblasts, suggesting that these cells do not represent a useful model to study the oxidative dysfunction associated with SALS.

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Blood oxidative stress in amyotrophic lateral sclerosis

Cited by 89 publications

References 38 publications

Oxidative stress biomarkers in sporadic ALS

Oxidative stress biomarkers in sporadic ALS

The Glutamate Hypothesis in ALS: Pathophysiology and Drug Development

Lack of Evidence for Oxidative Stress in Sporadic Amyotrophic Lateral Sclerosis Fibroblasts

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