Bleomycin sulphate loaded nanostructured lipid particles augment oral bioavailability, cytotoxicity and apoptosis in cervical cancer cells

Saini, Jyoti; Bansal, Vikas; Chandra, Ankush; Madan, Jitender; Jain, Upendra Kumar; Chandra, Ramesh; Jain, Sarvesh Malviya

doi:10.1016/j.colsurfb.2014.03.036

Cited by 24 publications

(10 citation statements)

References 49 publications

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“…While the diffractograms of Ed-CH-5-FU-Cel-MSs demonstrated, low intensity peaks designated to the amorphous lattice. This was consistent with the previous reports [33].…”

Section: Pxrd Studiessupporting

confidence: 94%

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Colon Specific Delivery of Combination of 5-Fluorouracil and Celecoxib: Preparation, Characterization, and in Vitro Cytotoxicity Assay

Bansal

Kamboj

Madan

2019

Asian J Pharm Clin Res

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Objective: 5-Fluorouracil (5-FU) and celecoxib (Cel) combination offered additive effect in the treatment of colon cancer. However, physicochemical and biopharmaceutical attributes of both drugs deliver suboptimal concentration at the site of action. The objective of the current study is the development of a microparticulate drug delivery system loaded with a combination of 5-FU and Cel to achieve prolonged drug delivery in colon cancer. Methods: 5-FU and Cel combination were loaded in Eudragit coated chitosan (CH) microspheres (MSs) and characterized. Results: The average particle size of the MSs was in the range of 2.7±0.9μm to 4.8±1.1μm. A substantial drug encapsulation efficiency of 71.30±2.3% as obtained for 5-FU as compared to 35.20±1.9% of Cel in the tailored microparticles. The drug loading capacity of 6.5 mg/10 mg and 2.3 mg/10 mg was obtained for 5-FU and Cel, respectively. By Eudragit S 100 (Ed) coating, significant pH-dependent release profile was achieved, and no drug release was observed in simulated gastric and intestinal fluids. The developed MSs exhibited the release of 92.1±2.9% of 5-FU in 8h whereas 18.9±0.7% Cel was found to be released from the developed MSs. The drug-loaded MSs exhibited appreciable potency against HT-29 cells with an IC50 value of 35.9 μM. Conclusion: The results indicated that these microparticles are a promising vehicle for selectively targeting drugs to the colon in the chemotherapy of colon cancer.

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“…While the diffractograms of Ed-CH-5-FU-Cel-MSs demonstrated, low intensity peaks designated to the amorphous lattice. This was consistent with the previous reports [33].…”

Section: Pxrd Studiessupporting

confidence: 94%

“…The HT 29 cells were lysed and thus formed formazan crystals were dissolved in 100 µL of DMSO and quantified by ELISA reader at 570 nm and 630 nm. The experiments were performed in triplicate (n=3)[31][32][33].…”

mentioning

confidence: 99%

Colon Specific Delivery of Combination of 5-Fluorouracil and Celecoxib: Preparation, Characterization, and in Vitro Cytotoxicity Assay

Bansal

Kamboj

Madan

2019

Asian J Pharm Clin Res

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“…They also anticipated that, having good biocompatibility and trafficking of cell membrane properties, the nanoparticles can be further used for numerous biomedical applications. Bleomycin sulfate-loaded nanostructured lipid particles resulted in improved oral bioavailability by avoiding first-pass metabolism and thereby upsurges in intestinal lymphatic uptake of drug to reach systemic circulation, finally leading to greater toxicity and apoptosis against cervical cancer cells [54]. Doxorubicin-encapsulated long circulating self-assembled nanoparticles prepared using amphiphilic brush-like block copolymer composed of polynorbonene-cholesterol/poly(ethylene glycol) were found to display considerably greater inhibition of tumor growth as compared with free doxorubicin and it was concluded that the prepared nanoparticles would be a valuable carrier for improving tumor drug delivery [55].…”

Section: Systemic Versus Localized Drug Delivery Systemsmentioning

confidence: 99%

“…No.DrugType/composition of nanoparticlesCell lines/animal models/neoplasmReferences1.DocetaxelPoly( ɛ -caprolactoneco-lactide)-D-α-tocopheryl polyethylene glycol succinate nanoparticlesCervical cancer cells[50]2.DocetaxelColic acid-PLGA-b-Vitamin E TPGS copolymer–[51]3.CisplatinFolic acid-conjugated gelatin nanoparticles–[52]4.–Phenanthridinium (oligonucleotide intercalator)-functionalized mesoporous silica nanoparticlesHeLa cells[53]5.Bleomycin sulfateNanostructured lipid particlesCervical cancer cells[54]6.DoxorubicinPolynorbonene-cholesterol/poly(ethylene glycol)–[55]7.DoxorubicinMesoporous silica nanoparticlesHeLa cells[56]8.DocetaxelD-α-tocopheryl polyethylene glycol 1000 succinate-b-poly(ε-caprolactone-ran-lactide) nanoparticlesXenograft BALB/c nude mice tumor model[57]9.DoxorubicinCyclodextrin-containing pH-sensitive poly(2-(dimethylamino) ethyl methacrylate) star polymer nanoparticles–[58]10.PaclitaxelPoly(γ-glutamic acid-maleimide-co-l-lactide)-1,2-dipalmitoylsn-glycero-3-phosphoethanolamine (γ-PGA-MAL-PLADPPE) copolymer-based nanoparticles–[59]11.DocetaxelPoly(lactide-co-glycolide) and pluronic F68C...…”

Section: Systemic Versus Localized Drug Delivery Systemsmentioning

confidence: 99%

Possible role of nanocarriers in drug delivery against cervical cancer

Gupta

2017

Nano Reviews & Experiments

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Introduction: Cervical cancer is the second most common cancer and the largest cancer killer among women in most developing countries including India. Although, various drugs have been developed for cervical cancer, treatment with these drugs often results in a number of undesirable side effects, toxicity and multidrug resistance (MDR). Also, the outcomes for cervical cancer patients remain poor after surgery and chemo radiation. Methods: A literature search (for drugs and delivery systems against cervical cancer) was performed on PubMed and through Google. The present review discuss about various methods including its current conventional treatment with special reference to recent advances in delivery systems encapsulating various anticancer drugs and natural plant products for targeting towards cervical cancer. The role of photothermal therapy, gene therapy and radiation therapy against cervical cancer is also discussed. Results: Systemic/targeted drug delivery systems including liposomes, nanoparticles, hydrogels, dendrimers etc. and localized drug delivery systems like cervical patches, films, rings etc. are safer than the conventional chemotherapy which has further been proved by the several drug delivery systems undergoing clinical trials. Conclusion: Novel approaches for the aggressive treatment of cervical cancer will optimistically result in decreased side effects as well as toxicity, frequency of administration of existing drugs, to overcome MDR and to increase the survival rates.

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“…Recently, various targeted drug delivery systems have received attention for their ability of delivering drugs to cancer tissues in a highly selective manner without affecting other tissues. Such systems include nanoparticles [8], nanostructured lipid particles [9], liquid crystals (LC) [10], pH-responsive micelles [11], etc. LC have the ability to form an organized mesophase exhibiting both crystalline solid and liquid properties [12].…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Curcumin Liquid Crystal Systems for Cervical Cancer

et al. 2020

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Curcumin is a hydrophobic compound with good anti-proliferative, anti-oxidative, and anti-cancer properties but has poor bioavailability. Liquid crystals (LC) can accommodate both hydrophilic and hydrophobic drugs. The aim of this study was to formulate and evaluate a novel vaginal drug delivery system for cervical cancer using a curcumin LC system. The curcumin LC system was formulated using surfactant, glycerol, and water together with curcumin. Three types of surfactants were used to optimize the formulation, i.e., Tween 80, Cremphor EL, and Labrasol. The optimized formulations were subjected to physicochemical analysis, and their efficacy was evaluated in HeLa cells. The pH of the formulations was in the range of 3.91–4.39. Environmental scanning electron microscopy (ESEM) observations revealed spherical as well as hexagonal micelles. In vitro release of LC curcumin from vaginal simulated fluid (VSF, pH 4.5) showed a release from 20.47% to 87.25%. The IC50 of curcumin in HeLa cells was 22.5 μg/mL, while the IC25 and IC75 were 6.5 μg/mL and 35μg/mL, respectively. The cytotoxicity of the formulations was determined in comparison with liquid crystals without curcumin and pure curcumin by performing a t-test based on a significance level of p less than or equal to 0.05 (p ≤ 0.05). The curcumin LC system was able to release the required amount of drug and was effective against the cervical cancer cell line examined.

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Bleomycin sulphate loaded nanostructured lipid particles augment oral bioavailability, cytotoxicity and apoptosis in cervical cancer cells

Cited by 24 publications

References 49 publications

Colon Specific Delivery of Combination of 5-Fluorouracil and Celecoxib: Preparation, Characterization, and in Vitro Cytotoxicity Assay

Colon Specific Delivery of Combination of 5-Fluorouracil and Celecoxib: Preparation, Characterization, and in Vitro Cytotoxicity Assay

Possible role of nanocarriers in drug delivery against cervical cancer

Development and Evaluation of Curcumin Liquid Crystal Systems for Cervical Cancer

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