Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia

Ramírez, Juan David; Flórez, Carolina; Olivera, Mario Javier; Bernal, María Consuelo; Giraldo, Julio Cesar

doi:10.1371/journal.pone.0172586

Cited by 68 publications

(98 citation statements)

References 38 publications

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“…Some studies not conducted in Latin America have found a low prevalence of Blastocystis in scenarios where there are alterations in the microbiota (such as those that may occur in inflammatory disease or Crohn's disease) when compared with a healthy population [3,21]. In the case of Colombia, cases of coinfection of Blastocystis with other intestinal protists have been reported, and there has been suggested zoonotic transmission of some subtypes of Blastocystis [22,23].…”

Section: Frequencies Of Coinfection Suggest a Possible Adaptation Of mentioning

confidence: 99%

Occurrence of Blastocystis in Patients with Clostridioides difficile Infection

et al. 2020

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Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread.

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Section: Frequencies Of Coinfection Suggest a Possible Adaptation Of mentioning

confidence: 99%

Occurrence of Blastocystis in Patients with Clostridioides difficile Infection

et al. 2020

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“…In America, there is a high prevalence of ST1 and ST2, in Australia, Europe, and South Eastern Asia -ST1 and ST3, and in Europe -ST4 [10]. A recent study from SouthAmerica showed the presence of ST4 in humans [47] and latter STs 1, 2 and 3 were detected as the most frequent [48]. People are colonized mainly by ST1 through ST4, containing over 90% of assessments; but depending on the regions and countries, infection by ST5 through ST9 is also detected [10,20].…”

Section: Pathogenicity Of Blastocystis Sp Is a Relationship Possiblementioning

confidence: 99%

Genetic diversity and pathogenicity of Blastocystis

Skotarczak¹

2018

Ann Agric Environ Med.

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Blastocystis is a unicellular, anaerobic protist which lives in the intestinal tract of diverse animals, including humans. It was found that the host specificity and the pathogenic potential of different isolates are correlated with sequence variations in the SSU-rRNA gene. Identification of the organism to the species level is still an unresolved challenge. Genetic diversity revisions have led to the identification of 17 subtypes (STs) within the Blastocystis genus, and 9 (ST1 to ST9) have been reported in humans with varying prevalence. Since the members of the genus revealed a large genetic diversity, several molecular modalities of subtyping methods have been developed. Numerous studies on conveying the pathogenic potential to the molecular subtypes are available, but they could not be compared or analysed with the different molecular techniques employed. The use of different approaches may give false positives during diagnosis and the possibility of missed infections. A review of recent scientific literature indicates that the development of PCR assays is needed for molecular epidemiology and for mixed infections in health and disease cohorts, and also to help identify sources of Blastocystis transmission to humans, as well as to identify potential animal and environmental reservoirs. This review summarizes some of the recent progress and improvements in Blastocystis research on genetic diversity, taxonomy, molecular epidemiology, pathogenicity and subtyping methods.

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“…Ramírez et al, 2017) in Colombia as only 0.8% were positive for both B. hominis and H. nana. On the contrary,(Licea et al, 2003) found that both B. hominis and H. nana were detected in 8%.…”

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confidence: 97%

Blastocystis Hominis infection and Its Relation with Other Intestinal Parasites among Outpatients, Sohag, Egypt

Elnazer

Shatat

OMRAN

et al. 2017

Sohag Medical Journal

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Intestinal parasites have a worldwide distribution, mainly in regions with precarious socio-economic and hygiene conditions. Blastocystis spp. is an anaerobic enteric protozoan inhabits human intestinal tract. Blastocystis hominis elicits a big controversy about its pathogenicity and its relation to bowel disease conditions. Our aim for this work is to determine the frequency of Blastocystis hominis infection among outpatients attending Ministry of Health hospitals and units in Sohag City and if there is a relation between Blastocystis hominis and other intestinal parasitic infections. The present research involved 150 cases from outpatient clinics of randomly selected hospitals and units in Sohag City from June 2016 to January 2017. Fresh stool samples were gathered and examined. Thirty-three samples (22%) were positive for parasitic infection, of whome, only 25 cases (75.8%) were positive for B. hominis whether single or mixed with other parasites. Single B. hominis infection represented 36% of positive cases. Out of 25 cases infected with B. hominis, 4 cases had mixed infection with G. lamblia (16%) (P-value = 0.027) and 10 cases had combined infection with E. coli (40%) (P-value < 0.001). In conclusion, 33 cases had parasitic infections of whom, 25 cases were positive for B. hominis. G. lamblia and E. coli were more common with B. hominis than other parasitic infection. This might be attributed to symmetry in genetic characters or the need for the same environmental conditions. To confirm this relation and its reasons we need further investigations.

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Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia

Cited by 68 publications

References 38 publications

Occurrence of Blastocystis in Patients with Clostridioides difficile Infection

Occurrence of Blastocystis in Patients with Clostridioides difficile Infection

Genetic diversity and pathogenicity of Blastocystis

Blastocystis Hominis infection and Its Relation with Other Intestinal Parasites among Outpatients, Sohag, Egypt

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