2008
DOI: 10.1016/s1472-6483(10)60308-2
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Abstract: Spermatozoa selection at high magnification before intracytoplasmic sperm injection seems to be positively associated with pregnancy rates after day 3 embryo transfers. The aim was to demonstrate an association between the presence of vacuoles in sperm nuclei and the competence of embryos to develop to day 5. Grading of spermatozoa at x 6000-x 12,500 magnification: grade I, no vacuoles; grade II, or=1 large vacuole; grade IV, large vacuoles with other abnormalities. The outcom… Show more

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Cited by 204 publications
(208 citation statements)
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“…Moreover, we could not find any deviations of early morphokinetics after fertilization with class I or class II/III spermatozoa. These observations confirm previous findings suggesting that the grade of sperm head vacuolization has no impact on the embryo outcome on day 3 [22,28,33,55]. Thus, the value of embryo selection prior to EGA remains questionable as the real developmental potency of the embryo cannot be properly estimated.…”
Section: Resultssupporting
confidence: 85%
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“…Moreover, we could not find any deviations of early morphokinetics after fertilization with class I or class II/III spermatozoa. These observations confirm previous findings suggesting that the grade of sperm head vacuolization has no impact on the embryo outcome on day 3 [22,28,33,55]. Thus, the value of embryo selection prior to EGA remains questionable as the real developmental potency of the embryo cannot be properly estimated.…”
Section: Resultssupporting
confidence: 85%
“…With regard to the reproductive outcomes in terms of fertilization, embryo development, pregnancy, and abortion rates, the importance of selecting normal spermatozoa becomes obvious when comparing oocyte injections performed with morphologically normal sperm to those injections with spermatozoa exhibiting different subcellular defects. Based on several studies, it is well documented that fertilization with spermatozoa revealing large nuclear vacuoles and/or abnormal shape of spermatozoa reduces the percentage of good-quality blastocysts on day 5 [18,[28][29][30]. However, the main limitation of the herepresented study is the low case number which is due to our policy to avoid fertilization with poor-quality sperm and the neglecting of the implementation of further female characteristics.…”
Section: Discussionmentioning
confidence: 81%
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“…The process of finding spermatozoa without vacuoles is difficult and time-consuming [7,8,15,16,[30][31][32][33]. In addition, switching between the glass-bottomed dish that is appropriate for Nomarski microscopy and the plastic-bottomed dish that is appropriate for Hoffman modulation contrast requires additional time, delaying the injection procedure [16].…”
Section: Discussionmentioning
confidence: 99%