Bis-aryl methanone compound is a candidate of nitric oxide producing elicitor and induces resistance in Nicotiana benthamiana against Phytophthora infestans

Defense induced by a bis-aryl methanone compound leads to resistance in potato against Phytophthora infestans

“…In the present situation concerning the NO-eliciting synthetic bis-aryl methanone compound, NUBS-4190, NO generation was triggered in potato ( Fig. 2) similar to our previous study, in which NUBS-4190 induced NO-mediated resistance against P. infestans in N. benthamiana (Monjil et al 2013). NO induction by NUBS-4190 in potato is sharp and reproducible, and NUBS-4190 could be a good synthetic compound for NO induction in potato.…”

“…3, 4). In our previous study (Monjil et al 2013), NUBS-4190 induced NO but not ROS in N. benthamiana. These results indicate that potato and N. benthamiana and/or the signal pathways responsible for ROS production may differ in their sensitivity to NUBS-4190.…”

“…Zoosporangia were collected, and zoospore production was induced by the methods of Doke (1975). Zoosporangia suspensions were prepared following the method of Monjil et al (2013); the isolate was subcultured on rye medium for 7-10 days, then 20 mL of water was added to the surface of the colonies, which were then rubbed with a cotton swab to release the zoosporangia. NUBS-4190-pretreated intact leaves were inoculated with a 1 mL sample of P. infestans zoospores (2 9 10 5 zoospores/mL) and covered with lens paper to keep the suspension of zoospores on the surface of the leaves.…”

“…NO was detected using the method described by Monjil et al (2013). Suspension cultured potato cells were collected and washed with assay buffer (5 mM MES-KOH, pH 5.7, containing 175 mM mannitol, 0.5 mM CaCl 2 , 0.5 mM K 2 SO 4 ).…”

“…1). NUBS-4190 is a synthetic compound previously reported to elicit NO and induced resistance in the model plant N. benthamiana against Phytophthora infestans (Monjil et al 2013). Authors studied the defense role against pathogen, particularly to examine the effect of NUBS-4190 to ascertain early reactions and to evaluate the induced resistant reactions in potato plants and suspension cultured potato cells.…”