Bipartite molecular approach for species delimitation and resolving cryptic speciation of Exobasidium vexans within the Exobasidium genus

Chaliha, Chayanika; Kaladhar, Vemula Chandra; Doley, Robin; Verma, Praveen Kumar; Kumar, Aditya; Kalita, Eeshan

doi:10.1016/j.compbiolchem.2021.107496

Cited by 5 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Light microscopy has been used for disease diagnosis in tea since the blister blight pathogen was first identified in 1898. Fluorescence microscopy and electron microscopy have been used to study the infection process of E. vexans [10] and to study the difference between E. vexans infected and healthy tea leaves [11]. Fluorescence microscopy using calcofluor white and wheat germ agglutinin stains and scanning electron microscopy of E. vexans inoculated tea leaves has been successfully used as a tool to study and diagnose the germination and infection stages of the pathogen [10].…”

Section: Microbiological and Microscopic Methodsmentioning

confidence: 99%

“…Hence, universal primers are used in most studies investigating the molecular identification and diversity of tea pathogens. Using the 28SrRNA sequence of E. vexans available in the NCBI GenBank, Chaliha et al [10] developed a species-specific primer pair for the selective amplification of E. vexans (EVLSUF-EVLSUR). However, the combination of universal forward primer ITS1F and species-specific reverse primer EVLSUR allows for the identification of multiple strains, resulting in a larger PCR product.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Disease Diagnosis in Tea (Camellia sinensis (L.) Kuntze): Challenges and the Way Forward

Devi Sinniah,

Mahadevan

2024

Challenges in Plant Disease Detection and Recent Advancements

View full text Add to dashboard Cite

Derived from the Camellia sinensis (L.) Kuntze plant, tea is the most widely consumed natural beverage in the world. Tea is a perennial woody plant. Monoculturing tea on a large scale makes it susceptible to many perennial and seasonal diseases. The leaves, stems, and roots of tea plants are susceptible to fungal, bacterial, and viral pathogens. Tea is predominantly grown in Asian and African regions; hence, conventional methods including symptomology and signs, and microbiological and microscopic methods are mainly used in disease diagnosis and pathogen identification. Accurate and rapid identification of diseases and pathogens is imperative for the sustainability of tea plantations. Thus, the technological advancement in plant disease diagnosis also embraces the global tea industry. This chapter discusses new technological advances in tea disease diagnosis, focusing on molecular biological methods, whole genome sequencing, and remote sensing and image analysis methods. Further, it highlights the challenges in disease diagnosis as a perennial woody plant and pins down available opportunities that could be successfully adopted to overcome the issues.

show abstract

Section: Microbiological and Microscopic Methodsmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%