Biotransformation of hydrocortisone by a natural isolate of Nostoc muscorum

Yazdi, Mojtaba Tabatabaei; Arabi, Homeira; Faramarzi, Mohammad Ali; Ghasemi, Younes; Amini, Mohsen; Shokravi, Shadman; Mohseni, Farzaneh Aziz

doi:10.1016/j.phytochem.2004.05.015

Cited by 32 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 and 9. Inspection of these spectra shows that characteristic bands of ingredients alone are consistent with those reported in the literature [30][31][32][33][34]. There are characteristic bands of hydrocortisone due to stretching vibrations of the O-H groups, C-H bonds, C=O groups and C-OH bonds over the ranges 3650-3100, 3100-2800, 1850-1540 and 1260-1000 cm -1 , respectively.…”

Section: Dsc Ir and Xrdp For Verification Of Resultssupporting

confidence: 86%

Compatibility studies of hydrocortisone with excipients using thermogravimetric analysis supported by multivariate statistical analysis

Rojek

Wesołowski

2016

J Therm Anal Calorim

View full text Add to dashboard Cite

Application of thermogravimetry (TG) alone to study compatibility/incompatibility of active pharmaceutical ingredients (APIs) with excipients yields to misleading results due to overlapping of the thermal stages in the course of decomposition of both ingredients and their pharmaceutical mixtures. Hence, the purpose of this study was to assess the usefulness of multivariate statistical analysis as a supporting tool for interpretation of the TG traces during assessing compatibility of hydrocortisone as an API with selected excipients (mannitol, starch, lactose, methylcellulose, b-cyclodextrin, meglumine, chitosan, magnesium stearate and polyvinylpyrrolidone). The results show that two multivariate techniques, principal component analysis (PCA) and cluster analysis (CA), can be successfully used for interpretation of TG traces, while the TG is used alone as a screening technique to assess compatibility. The results obtained by using TG analysis, supported by PCA and CA, were approved by those of differential scanning calorimetry, infrared spectroscopy and X-ray powder diffraction. Incompatibilities were only found in mixtures of hydrocortisone with magnesium stearate and b-cyclodextrin.

show abstract

Section: Dsc Ir and Xrdp For Verification Of Resultssupporting

confidence: 86%

Compatibility studies of hydrocortisone with excipients using thermogravimetric analysis supported by multivariate statistical analysis

Rojek

Wesołowski

2016

J Therm Anal Calorim

View full text Add to dashboard Cite

show abstract

“…Previtera group also examined the ability of transformation of ADD by the green alga T76 Scenedesmus quadricauda (Della Greca et al, 1996c). In a recent paper, hydrocortisone was partly converted with the cyanobacterium Nostoc muscorum PTCC 1636 into some androstane and pregnane products (Tabatabaei Yazdi et al, 2004). 17 and/or 20-carbonyl reduction and steroid side chain cleavage were the main alterations which occurred in hydrocortisone biotransformation.…”

Section: Resultsmentioning

confidence: 99%

“…Nostoc muscorum PTCC 1636 (Tabatabaei Yazdi et al, 2004) was maintained at 4 ºC on BG-11 (Borowitzka, 1988) agar slant and freshly subcultured before use in transformation experiments. The organism was transferred to fresh medium every two months.…”

Section: Instrumental Analysesmentioning

confidence: 99%

“…A mutational engineering of N. muscorum was studied for resistance to growth-inhibitory of LiCl and NaCl (Bhargava et al, 2003). We also reviewed through the literatures the production of numerous enzymes as well as the ability of steroid biotransformations by different microalgae including Nostoc muscorum in our previous paper (Tabatabaei Yazdi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nostoc muscorum: a regioselective biocatalyst for 17-carbonyl reduction of androst-4-en-3,17-dione and androst-1,4-dien-3,17-dione

et al. 2006

View full text Add to dashboard Cite

Nostoc muscorum PTCC 1636 was examined for its ability to convert androst-4-en-3,17-dione (AD) and androst-1,4-dien-3,17-dione (ADD) to their 17-hydroxy related derivatives in BG-11 medium. Bioconversion procedures were carried out at 25 ºC without shaking. The metabolites obtained were purified using chromatographic methods and characterized as testosterone and 1-dehydrotestosterone on the basis of their spectroscopic features. In both cases, the bioreaction characteristics observed were 17-carbonyl reduction.

show abstract

“…Some important enzymes including hydrogenase (Tamagnini et al 2002) and ornitine transcarbamoylase (Wolk et al 1994) have been isolated from this microorganism. Two recent studies have described the regio-selective conversion of hydrocortisone, androst-4-en-3,17-dione, and androst-1,4-dien-3,17-dione by N. muscorum (Tabatabaei Yazdi et al 2004;Faramarzi et al 2006). In addition, organic mono-phase and organic-aqueous twophase systems have been successfully used to improve the conversion yield of steroid substrates by this cyanobacterium (Arabi et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of codeine to semi-synthetic opiate derivatives by the cyanobacterium Nostoc muscorum

Niknam

Faramarzi

Abdi

et al. 2009

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Very limited studies have been done to investigate the algal biotransformation of codeine to its opioid derivatives. On the other hand, microalgae have been recently introduced as potential tools for green synthesis of various organic compounds. In the present work, the capability of biotransformation of codeine by a locally isolate strain of cyanobacterium, Nostoc muscorum, was evaluated. Incubation of the whole cells of Nostoc muscorum with codeine (I) under continuous light photoregime of 60 lmol photons/m 2 s at 25°C for 5 days gave rise to four transformation products. The bioproducts were separated by gas chromatography and identified as 6-acetylcodeine (II), oxycodone (III), norcodeine (IV), morphine (V) and based on their mass spectra. Observed modifications included O-demethylation, N-demethylation, C6-acetylation, C14-hydroxilation, D 7 -reduction, and C6-oxidation. The ability of N. muscorum to convert codeine to oxycodone (III) represents an uncommon pattern of codeine metabolism in microorganisms that may be of industrial importance.

show abstract

Biotransformation of hydrocortisone by a natural isolate of Nostoc muscorum

Cited by 32 publications

References 35 publications

Compatibility studies of hydrocortisone with excipients using thermogravimetric analysis supported by multivariate statistical analysis

Compatibility studies of hydrocortisone with excipients using thermogravimetric analysis supported by multivariate statistical analysis

Nostoc muscorum: a regioselective biocatalyst for 17-carbonyl reduction of androst-4-en-3,17-dione and androst-1,4-dien-3,17-dione

Bioconversion of codeine to semi-synthetic opiate derivatives by the cyanobacterium Nostoc muscorum

Contact Info

Product

Resources

About