Biotransformation of citral and nerol by spores ofPenicillium digitatum

Abstract

A simple and efficient method was developed to carry out biotransformation reactions on terpenoid compounds. For these experiments spores of Penicillium digitatum were inoculated on solid media in conical flasks. After a short incubation period, the spores germinated and a mycelial culture was formed. After 2 weeks, the cultures had completely sporulated and bioconversion reactions were started. For this purpose a known volume of nerol or citral was sprayed on to the sporulated surface culture. After 1–2 days, a period during which transformation took place, the flasks were fitted with an aeration piece, connected with a tube filled with Tenax. The bioconversion was monitored by dynamic headspace techniques during a period of 8, 24 or 48 hours. Both nerol and citral were transformed into 6‐methylhept‐5‐en‐2‐one by sporulated surface cultures. The spores retained their activity over prolonged periods, up to 2 months. The overall yield varied from 92% to 99%. © 1998 John Wiley & Sons, Ltd.

Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

“…Microbial resting forms are much more insusceptible to external factors, e.g., toxicity of chemical compounds, temperature or desiccation [22,52,53]. Therefore, in some cases they can successfully replace other, more sensitive forms of the biocatalyst (vegetative ones) [52,54,55]. Fungal spores are a source of specific, for this form, enzymatic activities [56].…”

Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

“…Microbial resting forms are much more insusceptible to external factors, e.g., toxicity of chemical compounds, temperature or desiccation [22,52,53]. Therefore, in some cases they can successfully replace other, more sensitive forms of the biocatalyst (vegetative ones) [52,54,55]. Fungal spores are a source of specific, for this form, enzymatic activities [56].…”

Toxicity of terpenes to spores and mycelium of Penicillium digitatum

“…Organism P. digitatum ATCC 201167 (P. digitatum CLE) was isolated from a spoiled tangerine (Demyttenaere and De Pooter, 1998). The culture was maintained as a spore suspension in buffer (see below) stored at −80°C.…”

Methods of Biotechnology in the Synthesis of Drugs (2nd Ed.)

“…Известно, что грибы из родов Aspergillus и Penicillium могут трансформировать цитраль [Larroche, Gros, 1989;Demyttenaere, De Pooter, 1998;Demyttenaere et al, 2000]. Например, растущая культура гриба Penicillium sp.…”

Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

“…Microbial resting forms are much more insusceptible to external factors, e.g., toxicity of chemical compounds, temperature or desiccation [22,52,53]. Therefore, in some cases they can successfully replace other, more sensitive forms of the biocatalyst (vegetative ones) [52,54,55]. Fungal spores are a source of specific, for this form, enzymatic activities [56].…”

Toxicity of terpenes to spores and mycelium of Penicillium digitatum

“…Organism P. digitatum ATCC 201167 (P. digitatum CLE) was isolated from a spoiled tangerine (Demyttenaere and De Pooter, 1998). The culture was maintained as a spore suspension in buffer (see below) stored at −80°C.…”

Methods of Biotechnology in the Synthesis of Drugs (2nd Ed.)

“…Известно, что грибы из родов Aspergillus и Penicillium могут трансформировать цитраль [Larroche, Gros, 1989;Demyttenaere, De Pooter, 1998;Demyttenaere et al, 2000]. Например, растущая культура гриба Penicillium sp.…”

Half-Preparative Scale Synthesis of (S)-1-Phenylethane-1,2-Diol as a Result of 2-Phenylethanol Hydroxylation with Aspergillus niger (IAFB 2301) Assistance

“…Microbial resting forms are much more insusceptible to external factors, e.g., toxicity of chemical compounds, temperature or desiccation [22,52,53]. Therefore, in some cases they can successfully replace other, more sensitive forms of the biocatalyst (vegetative ones) [52,54,55]. Fungal spores are a source of specific, for this form, enzymatic activities [56].…”

Toxicity of terpenes to spores and mycelium of Penicillium digitatum

“…Organism P. digitatum ATCC 201167 (P. digitatum CLE) was isolated from a spoiled tangerine (Demyttenaere and De Pooter, 1998). The culture was maintained as a spore suspension in buffer (see below) stored at −80°C.…”

Methods of Biotechnology in the Synthesis of Drugs (2nd Ed.)

“…Известно, что грибы из родов Aspergillus и Penicillium могут трансформировать цитраль [Larroche, Gros, 1989;Demyttenaere, De Pooter, 1998;Demyttenaere et al, 2000]. Например, растущая культура гриба Penicillium sp.…”