Biotin Interference in Thyroid Panel Assays With Biotinylated Components

Root, Matthew; Karger, Amy B.; Killeen, Anthony A.; Larson, Kathy; Sokoll, Lori J.; Li, Danni

doi:10.1093/ajcp/aqw191.018

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“…Biotin Interference in Clinical Immunoassays observed interference at any biotin concentration for β-human chorionic gonadotropin, LH, ferritin, myoglobin, CA15.3, CA19.9, free PSA, and total PSA assays (26). In a second study, TSH, free T 4 , and free T 3 were shown to be altered at biotin concentrations of 320 μg/L, 320 μg/L, and 160 μg/L, respectively, on the Dimension Vista, while there was no change at any biotin concentration for total T 3 and total T 4 (21).…”

Section: Mini-reviewmentioning

confidence: 93%

Biotin Interference in Clinical Immunoassays

Colon

Greene

2018

The Journal of Applied Laboratory Medicine

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Background: Shifts in consumer behavior and clinical practice have increased reports of biotin interference in immunoassays that incorporate biotin-streptavidin linkages in their design. This rise has significant implications because immunoassays are used to measure a wide range of analytes across medical specialties. The objective of this article was to review the mechanisms of biotin interference, define the scope of affected immunoassays, and summarize strategies for identifying and minimizing biotin interference in clinical samples. Content: The literature was reviewed for reports of biotin interference and sorted by manufacturer. Abstracts from recent scientific meetings and manufacturer-provided data were also reviewed. Summary: Immunoassays across a range of different manufacturer platforms are subject to interference by exogenous biotin supplementation. Providers and laboratorians must be aware of this potential interference to avoid patient harm.

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Section: Mini-reviewmentioning

confidence: 93%