Biotic elicitation for enhanced production of plumbagin in regenerated shoot cultures of Plumbago zeylanica using response surface methodology

Katoch, Kajal; Gupta, Suphla; Gupta, Ajai Prakash; Goyal, Pooja; Devi, Ritu; Dey, Abhijit; Pandey, Devendra Kumar

doi:10.1007/s11240-022-02375-5

Cited by 10 publications

(5 citation statements)

References 43 publications

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“…In addition, the elicitation procedure was convenient as it could be completed in a single step by culturing nodal explants in a culture medium containing an elicitor. Recent research has employed regenerated P. zeylanica shoots to enhance plumbagin production through elicitation [ 25 ]. Numerous plant species have bioactive compounds that accumulate in their field-grown roots or underground stems.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a number of elicitors have been used to enhance plumbagin yield in the genus Plumbago in either P. indica or P. zeylanica . Yeast extract and salicylic acid were chosen for this study due to their effectiveness as biotic elicitors in P. zeylanica [ 22 , 23 , 24 , 25 ]. Yeast extract is a dead microbe cell or fragment, whereas salicylic acid is a plant hormone.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.

Jirakiattikul,

Ruangnoo,

Sangmukdee

et al. 2024

Plants

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Plumbago indica L. contains a valuable bioactive compound called plumbagin. Elicited regenerated shoots grown in vitro could be another source of high-yielding plumbagin. The purpose of this investigation was to examine the effects of elicitor type and concentration, as well as elicitation period, on plumbagin content in in vitro-regenerated shoots of P. indica. Nodal explants were cultured on Murashige and Skoog (MS) medium containing 1 mg/L benzyladenine (BA) in combination with 0–150 mg/L yeast extract or 50–150 µM salicylic acid for four weeks. Plumbagin levels of 3.88 ± 0.38% and 3.81 ± 0.37% w/w g dry extract were achieved from the 50 and 100 mg/L yeast extract-elicited shoots, which were higher than the value obtained for the control. However, the addition of salicylic acid did not increase the plumbagin content. In the elicitation period experiment, nodal explants were cultured on MS medium supplemented with 1 mg/L BA and 50 mg/L yeast extract for durations of three, four and five weeks. The 4-week yeast extract-elicited shoot had a maximum plumbagin content of 3.22 ± 0.12% w/w g dry extract, greater than that of the control. In summary, the plumbagin content of the in vitro P. indica shoots was enhanced by 4-week elicitation using 50 mg/L yeast extract.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.

Jirakiattikul,

Ruangnoo,

Sangmukdee

et al. 2024

Plants

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“…Plumbago indica L.retained or even enhanced this property, which was consistent with the results of previous studies. In addition, it had also been suggested that enhanced antioxidant enzyme activity, expression of resistance genes, and production of defense signaling molecules could stimulate the production and accumulation of secondary metabolites ( Katoch et al., 2022 ), and in this study we screened cold-resistant mutants, and the original accumulation of plumbagin in the cold- resistant Plumbago indica L. mutants was greater than or equal to that of the control, suggesting that this the cold-resistant mutant, in addition to better mobilization of antioxidant enzyme activity, may also derive from its higher plumbagin content, which has better cold tolerance.…”

Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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Plumbago indica L. is a perennial herb with ornamental and anticancer medicinal functions widely distributed in the tropics. It is affected by temperature and cannot bloom normally in colder subtropical regions, which seriously affects its ornamental value. To create low-temperature resistance mutants and enrich new germplasm resources, this study used tissue culture and chemical reagent (0.5 mmol/L NaN3) and low-temperature stress (0°C, full darkness for 48h) induction to target and screen for cold-resistance mutants. The results showed that the ISSR band polymorphism ratio of the 24 suspected mutant materials was 87.5%. The DNA profiles of the 9 mutants initially identified were altered. The content of plumbagin in the stems and leaves of the mutants was examined, and it was found that the accumulation in the leaves of the mutant SA24 could be as high as 3.84 times that of the control, which was 0.5991%. There were significant differences in the anatomical structures of roots, stems and leaves. The mutants mostly exhibited reduced root diameter (only 0.17-0.69 times that of CK), increased stem diameter (up to 2.19 times that of CK), enlarged mesophyll cells, increased thickness (up to 1.83 times that of CK) and high specificity, which are thought to be important for the different cold resistance obtained by the mutants. In the cold resistance experiment, four cold-tolerant mutants were successfully screened according to their morphological characteristics and physiological indexes, and the mutagenesis efficiency could be as high as 2.22% and did not affect the accumulation of plumbagin in their stems and leaves, even higher than CK. The responses of the screened mutants SA15, SA19, SA23 and SA24 to low temperature showed slower leaf wilting, higher light energy conversion efficiency, less accumulation of MDA content, increased enzymatic activities of antioxidant enzymes (SOD, CAT, POD) and more accumulation of soluble sugars and proline content. These characteristics are consistent with the response of cold-resistance plants to low temperatures. The cold- resistance mutants cultivated in soil were observed of agronomic and ornamental traits for one year, mainly manifested as delayed flowering and delayed entry into the senescence stage. This study provides a more rapid and accurate technique for identifying and screening cold-tolerant mutants, and lays the foundation for future experiments on the creation of new cold-resistant varieties.

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“…It is well known that specialized metabolites take part of the adaptive phenotypically plastic responses of plants subjected to different types of stress. A recent example is the increment in the accumulation of the naphthoquinone plumbagin in in vitro shoot cultures of Plumbago zeylanica by elicitation with bacterial and fungal lysates in combination with yeast extract (Katoch et al 2022).…”

Section: Quantification Of Selected Biomarkersmentioning

confidence: 99%

In vitro plant growth regulator-free propagation of Hedeoma multiflora Beth. Comparison of exo-morphological and metabolomic aspects vs. wild populations.

Diaz-Gabutti

Magallanes-Noguera²,

Cecati

et al. 2023

Preprint

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The aromatic plant Hedeoma multiflora Beth. is endangered by both the overexploitation and the advance of agricultural and urban borders. We report an efficient protocol for its in vitro propagation without using plant growth regulators (PGRs). Two in vitro plant lines, one initiated from seeds and other from binodal segments, remain stable and present high multiplication rates in half strength Murashigue and Skoog medium. Neither the addition of 1-naphthylacetic acid, kinetin or their combinations improved either growing or multiplication rates which reached 26.39 stems per initial explant after 12 weeks. A comparison toward three wild populations, harvested at both early and late summer time, shows that the morpho-anatomical characteristics of the aerial parts are fairly preserved although in vitro plants display more abundant but shorter roots. The profiles of volatile compounds from wild and in vitro specimens are quite similar, although hydrocarbon monoterpenes’ fraction is more important in the latter. Pulegone is by far the main volatile metabolite in all the assessed populations while its proportion drops in the in vitro and in the wild plants from late summer respect to wild plants from December. The concentration of the biomarkers, menthone, isomenthone, pulegone and isopulegone, is about an order of magnitude lower in the in vitro than in the wild plants. Overall, the biomass production in a PGR-free medium ensures constant composition of specialised metabolites and absence of biological and chemical contaminations, representing an alternative for the sustainable use of H. multiflora as edible material.

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Biotic elicitation for enhanced production of plumbagin in regenerated shoot cultures of Plumbago zeylanica using response surface methodology

Cited by 10 publications

References 43 publications

Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.

Enhancement of Plumbagin Production through Elicitation in In Vitro-Regenerated Shoots of Plumbago indica L.

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

In vitro plant growth regulator-free propagation of Hedeoma multiflora Beth. Comparison of exo-morphological and metabolomic aspects vs. wild populations.

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