Biosynthesis of ZnONP Using Chamaecostus cuspidatus and Their Evolution of Anticancer Property in MCF-7 and A549 Cell Lines

Abstract

The ZnO nanoparticle synthesis using the leaf part of Chamaecostus cuspidatus was characterized using UV–Vis spectrophotometry, IR, XRD, DLS, FESEM, EDX, TEM, AFM and XPS. The MTT assay was used to examine the cytotoxicity activity against lung epithelial and breast cell lines, and the IC50 value was determined. The presence of ZnO nanoparticles, which range in size from 200 to 800 nm, was confirmed by the absorption peak at 350 nm. The median particle size was 145.1 nm, and the ζ -the potential was -19.45 mV, showing that ZnONP is stable. Zinc, carbon, and oxygen contribute to the elemental composition of ZnONP, as determined by EDX analysis. MTT assay was used to investigate in vitro cytotoxicity in MCF-7 and A549 cell lines. The cytotoxicity activity IC50 value was determined to be 30 μg/mL for the A549 cell line and 37 μg/mL for the MCF-7 cell line.

Green Synthesis, Characterization and Bioactivity of Mangifera indica Seed-Wrapped Zinc Oxide Nanoparticles

“…On the other hand, the phytochemicals that are present in the plant source are the ones that are thought to be responsible for the nanoparticles’ reduction and stabilization [ 14 , 46 ]. In agreement, we speculate in the present study that the phytochemicals, such as polyphenols, flavonoids, and carotenes found in the seed extract, might be deeply involved in the bio-reduction of the nanoparticles [ 42 , 43 ].…”

“…Meanwhile, peaks were shown with 2θ values of 31.4°, 34.0°, 35.9°, 47.1°, 56.2°, 62.5° and 68.7° which corresponds to (100), (002), (101), (102), (110), (103) and (200) respectively. ZnO NPs synthesized from Andrographis paniculate, Chamaecostus cuspidatus and Agathosma betulina showed an identical XRD pattern [ 33 , 41 , 42 ].…”

“…In this context, researchers have initiated to utilize the biowaste materials from various plant and fruit sources to synthesize metal-based and metallic oxide nanoparticles. Several ways for generating ZnO NPs utilizing various plant extracts have been established by researchers [ 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 ]. We report here on the large - scale production of ZnO NPs by using an aqueous extract of mango seed powder as the raw material.…”

“…The biosynthesis of ZnO NPs has been shown utilizing several plant extracts, including Lobelia leschenaultiana [ 32 ], Agathos mabetulina [ 33 ], Laurus nobilis [ 34 ], Moringa olifera [ 35 ], Acalypha indica [ 36 ], Aspalathus linearis [ 37 ], Carica papaya [ 38 ], green tea leaves [ 39 ], Euphorbia jatropa latex [ 40 ], Andrographis paniculata [ 41 ], Chamaecostus cuspidatus [ 42 ]. Compared to the above sources, the advantage of preparing ZnO NPs using mango seed extracts has been cost-effectiveness, as rinds and seeds are considered waste materials.…”