Biosynthesis of the Polycyclic System in the Antifungal HSAF and Analogues from <i>Lysobacter enzymogenes</i>

Li, Yaoyao; Wang, Haoxin; Liu, Yan; Jiao, Yujie; Li, Shanren; Shen, Yuemao; Du, Liangcheng

doi:10.1002/ange.201802488

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…1). 8, 9 The structure of HSAF differs remarkably from those of existing fungicides on the market 10 . Because of its significant bioactivities and distinct structure, great efforts have been made to investigate the biosynthetic and regulatory mechanisms of HSAF 8, 9, 11 .…”

Section: Introductionmentioning

confidence: 99%

“… 8, 9 The structure of HSAF differs remarkably from those of existing fungicides on the market 10 . Because of its significant bioactivities and distinct structure, great efforts have been made to investigate the biosynthetic and regulatory mechanisms of HSAF 8, 9, 11 . In our previous studies, strategies including genetic modification and fermentation optimization have been investigated in an effort to improve the production of HSAF in L. enzymogenes strain OH11 12‐15 .…”

Section: Introductionmentioning

confidence: 99%

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Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Tang

Cao

et al. 2020

Pest Management Science

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BACKGROUND Anthracnose caused by Colletotrichum fructicola is one of the most important diseases in pear fruit, resulting in huge economic losses. Public awareness of protecting the environment and food safety, together with pathogen resistance to many key fungicides have led to an urgent need to develop alternative strategies for controlling fruit diseases. Here, the antifungal activity of a natural product, dihydromaltophilin [heat‐stable antifungal factor (HSAF)], against C. fructicola in vitro and in vivo was investigated to determine its efficacy for anthracnose management. RESULTS HSAF exhibited pronounced antifungal activity against in vitro mycelial growth of C. fructicola, with a half‐inhibition concentration of 0.43 mg L−1. Hyphae treated with HSAF showed defects such as hyperbranching, swelling and depolarized growth. Conidia germination in the pathogen was inhibited by HSAF in a dose‐dependent manner. In the presence of 4 mg L−1 HSAF, conidia germination was significantly delayed, and germ tube growth was inhibited. HSAF at 8 mg L−1 completely blocked conidia germination in C. fructicola. In addition, HSAF disrupted coordination of cytokinesis with growth and nuclear division, induced reactive oxygen species production in conidia, and damaged the integrity of the conidia cell wall. Moreover, an in vivo test confirmed that 50 mg L−1 HSAF significantly reduced the development of anthracnose decay in pear fruit caused by C. fructicola. CONCLUSION HSAF was highly effective in reducing pear anthracnose caused by C. fructicola and has great potential to become a new type of fruit preservative.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Tang

Cao

et al. 2020

Pest Management Science

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“…7A) also indicates that these substances contain a heat-stable factor. The HSAF family has structural diversity (Li et al, 2018) and the BGC in S10 has 75% similarity to the reported HASF (BGC0000999). Thus the heatstable factor in Streptomyces S10 could be a new HASF and probably has another biosynthesis pathway or uncharacterized mechanism that is different from bacteria (Su et al, 2018).…”

Section: Secondary Metabolite Analysis Of S10mentioning

confidence: 95%

Antagonistic action of Streptomyces pratensis S10 on Fusarium graminearum and its complete genome sequence

Zhang

Chen

et al. 2020

Environmental Microbiology

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Wheatscab, mainly caused by Fusarium graminearum, can decrease wheat yield and grain quality. Chemical pesticides are currently the main control method but have an inevitable negative consequence on the environment and in food safety. This research studies a promising substitute, Streptomyces pratensis S10, which was isolated from tomato leaf mould and shows a significant inhibition effect on F. graminearum based on antagonism assays. The biocontrol mechanism is studied by enhanced green fluorescent protein labelling, quantitative real-time PCR, the Doskochilova 8 solvents system test and complete genome sequencing. Strain S10 can colonize in the wheat root, control wheat scab and decrease deoxynivalenol (DON) content. The control effects in vitro, planta and the plot experiments were 92.86%, 68.67% and 40.87% to 86.62%, respectively. S10 decreased DON content by inhibiting the mycelium growth and DON synthesis gene expression. The active substances of the S10 secondary metabolites had a high-temperature resistance and 29 putative biosynthetic gene clusters in its genome. The S10 control mechanism is multivariate, which shows potential in controlling wheat scab.

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“…Lysobacter enzymogenes is a nonpathogenic strain that was used to control crop fungal diseases known for the synthesis of an antifungal weapon (heat-stable antifungal factor, HSAF) that exhibits inhibitory activity against a wide range of fungal species [16][17][18][19][20][21][22][23] . Our previous work revealed that RpfG affects production of the antifungal weapon HSAF in L. enzymogenes 24 .…”

Section: Introductionmentioning

confidence: 99%

A predatory soil bacterium reprograms a quorum sensing signal system to regulate antifungal weapon production in a cyclic-di-GMP-independent manner

Wang

et al. 2021

Preprint

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Soil bacteria often provide multiple weapons for eukaryotes or prokaryotes to use against predators. Diffusible signal factors (DSFs) represent a unique group of quorum sensing (QS) chemicals that modulate interspecies competition in bacteria that do not produce antibiotic-like molecules. However, the molecular mechanism by which DSF-mediated QS systems regulate weapons production for interspecies competition remains largely unknown in soil biocontrol bacteria. In this study, we found that the necessary QS system component protein RpfG from Lysobacter, in addition to being a cyclic dimeric GMP (c-di-GMP) phosphodiesterase (PDE), regulates the biosynthesis of an antifungal weapon (heat-stable antifungal factor, HSAF), which does not appear to depend on the enzymatic activity. Interestingly, we showed for the first time that RpfG interacts with three hybrid two-component system (HyTCS) proteins, HtsH1, HtsH2, and HtsH3, to regulate HSAF production in Lysobacter. In vitro studies showed that each of these proteins interacted with RpfG, which reduced the PDE activity of RpfG. Finally, we showed that the cytoplasmic proportions of these proteins depended on their phosphorylation activity and binding to the promoter controlling the genes implicated in HSAF synthesis. These findings reveal a new mechanism of DSF signalling in antifungal weapon production in soil bacteria.

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Biosynthesis of the Polycyclic System in the Antifungal HSAF and Analogues from Lysobacter enzymogenes

Cited by 5 publications

References 25 publications

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Biocontrol ability and action mechanism of dihydromaltophilin against Colletotrichum fructicola causing anthracnose of pear fruit

Antagonistic action of Streptomyces pratensis S10 on Fusarium graminearum and its complete genome sequence

A predatory soil bacterium reprograms a quorum sensing signal system to regulate antifungal weapon production in a cyclic-di-GMP-independent manner

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