Biosynthesis of Acetylenic Fatty Acids in the Moss Ceratodon purpureus (Hedw.) Brid.

Kohn, Gerhard; Hartmann, E.; Stymne, Sten; Beutelmann, Peter

doi:10.1016/s0176-1617(11)81185-5

Cited by 23 publications

(29 citation statements)

References 14 publications

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“…It is clear from recent in-vivo studies that TAGs in a variety of plant tissues are subject to metabolic turnover during a cell stage of active oil deposition (Graces et al 1994;Kohn et al 1994;Khozin et al 1997. The in-vitro results presented here show that there are enzymatic reactions in plant membranes that can account for these in-vivo events.…”

Section: Discussionmentioning

confidence: 55%

“…GraceÂ s et al (1994) demonstrated that oleate from TAGs was desaturated to linoleate in developing sun¯ower seeds when these were transferred to a lower temperature. The moss Ceratodon purpurea desaturated 9,12-octadecadien-6-ynoic acid (an acetylenic acid present in the TAGs) at the D15 carbon (Kohn et al 1994). Two hypotheses could explain these results: (i) direct desaturation of acyl groups in TAGs and (ii) recycling of fatty acids from TAGs to other lipids and return of the modi®ed acyl groups back to TAGs.…”

Section: Introductionmentioning

confidence: 99%

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Remodelling of triacylglycerols in microsomal preparations from developing castor bean (Ricinus communis L.) endosperm

Mancha

Stymne

1997

Planta

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Microsomal preparations from developing castor bean (Ricinus communis L.) endosperm catalyzed remodelling of in-situ-formed triacylglycerol (TAG) species. Castor bean microsomal membranes synthesized [ 14 C]TAGs from either glycerol 3-phosphate and [ 14 C]ricinoleoyl-CoA or [ 14 C]glycerol 3-phosphate and ricinoleoyl-CoA. Upon repelleting and subsequent incubation of the microsomes a redistribution occurred of both the [ 14 C]glycerol and [ 14 C]ricinoleoyl moieties of the in-situ-synthesized [ 14 C]TAGs. Radioactivity was transferred from TAG species with three (3HO-TAG) or two (2HO-TAG)ricinoleoyl groups into species with two or one (HO-TAG) ricinoleoyl groups. Mass analysis of the lipid and fatty acid movements in the membranes showed that a net synthesis of TAGs with no, one and two ricinoleoyl groups occurred at the expense of 3HO-TAG and polar lipids. Thus, the non-hydroxylated acyl groups from polar lipids were used in the remodelling of TAGs. In-vivo feeding of [ 14 C]ricinoleic acid to slices of castor bean endosperm demonstrated the presence of two radioactive pools of TAGs one in the oil bodies, which was rich in [ 14 C]3HO-TAG, and one associated with the microsomal membranes, which was dominated by radioactive 1HO-TAG and 2HO-TAG. The microsomal TAG pool was remodelled in vivo in a similar way as in the in-vitro experiments with microsomal membranes. Abbreviations: DAG = diacylglycerol; G3P = sn-glycerol 3-phosphate; HO = ricinoleoyl group (the number in front of HO designates the number of ricinoleate molecules per molecule of lipid, (i.e. HO-TAG, 2HO-TAG and 3HO-TAG designate triacylglycerols with 1, 2 and 3 ricinoleoyl groups, respectively); HO-18:1 = free ricinoleic acid; MAG = monoacylglycerol; NE-FA = non-hydroxylated free fatty acids; PA = phosphatidic acid; PC = phosphatidylcholine; PE = phosphatidylethanolamine; TAG = triacylglycerol Correspondance

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Remodelling of triacylglycerols in microsomal preparations from developing castor bean (Ricinus communis L.) endosperm

Mancha

Stymne

1997

Planta

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“…[1-14 C]18:3 9c,12c,15c (2P) and [U-14 C] 18:4 6c,9c,12c,15c (6D) were converted to 18:4 6a,9c,12c,15c (6C) and EPA (6E) showing that the acetylenic bond is formed in a discrete step [83]. The sequence of desaturation events in moss was explored by Kohn [84]. When C. purpureus protonemata were incubated with radiolabeled precursors, it was demonstrated that γ-linolenate (6A) was converted to acetylenic fatty acid 6C and dehydrogenation of 6D led to 6C and tetraenoic acid 6E.…”

Section: Steariolic and Tariricmentioning

confidence: 99%

Biosynthesis and function of polyacetylenes and allied natural products

Minto

Blacklock

2008

Progress in Lipid Research

291

228

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Polyacetylenic natural products are a substantial class of often unstable compounds containing a unique carbon-carbon triple bond functionality, that are intriguing for their wide variety of biochemical and ecological functions, economic potential, and surprising mode of biosynthesis. Isotopic tracer experiments between 1960 and 1990 demonstrated that the majority of these compounds are derived from fatty acid and polyketide precursors. During the past decade, research into the metabolism of polyacetylenes has swiftly advanced, driven by the cloning of the first genes responsible for polyacetylene biosynthesis in plants, moss, fungi, and actinomycetes, and the initial characterization of the gene products.The current state of knowledge of the biochemistry and molecular genetics of polyacetylenic secondary metabolic pathways will be presented together with an up-to-date survey of new terrestrial and marine natural products, their known biological activities, and a discussion of their likely metabolic origins.

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“…For many years C. purpureus has been used a model to study physiology (Algarra et al, 1993;Kohn et al, 1994), population genetics (Skotnicki et al, 2004;McDaniel, 2005;McDaniel et al, 2007), and sexual ecology (Shaw & Gaughan, 1993;Shaw & Beer, 1999;Rosenstiel et al, 2012). Along with the recent effort to sequence its genome (http://jgi.doe.gov/why-sequence-ceratodonpurpureus-moss/), and an increasing understanding of its general ecology, C.…”

Section: Ceratodon Purpureusmentioning

confidence: 99%

Sex-Specific Fungal Communities of the Dioicous Moss Ceratodon purpureus

Balkan¹

2000

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Mosses display a number of hallmark life history traits that influence their ecology at the population and community level. The long lived separation of sexes observed in the haploid gametophyte (dioicy) is one such feature of particular importance, as it is observed in the majority of bryophytes and creates intraspecific specialization of male and female individuals.The prevalence of sexually dimorphic mosses raises the possibility of sex-specific interactions with fungi as observed in some vascular plants. Here I investigated how moss sex shapes fungal communities associated with gametophytic tissues of the ubiquitous moss, Ceratodon purpureus. Using greenhouse populations of C. purpureus grown in a common garden, I examined fungal community structure and overall abundance of fungal biomass associated with male and female individuals from multiple populations. I hypothesized that individual mosses would harbor unique fungal communities based on their sex, and that overall fungal biomass associated with host tissues would differ significantly due to differences in morphological and physiological characteristics between the sexes. I found that fungal community composition and overall abundance (i.e. biomass) differ between male and female individuals of C. purpureus, and that sex-specific patterns are retained across individuals from three different populations. This work provides a first glance at how genetically based sexual systems in early land plants influence affiliated fungal community composition.ii Acknowledgments

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Biosynthesis of Acetylenic Fatty Acids in the Moss Ceratodon purpureus (Hedw.) Brid.

Cited by 23 publications

References 14 publications

Remodelling of triacylglycerols in microsomal preparations from developing castor bean (Ricinus communis L.) endosperm

Remodelling of triacylglycerols in microsomal preparations from developing castor bean (Ricinus communis L.) endosperm

Biosynthesis and function of polyacetylenes and allied natural products

Sex-Specific Fungal Communities of the Dioicous Moss Ceratodon purpureus

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