Biosynthesis, characterization and acute oral toxicity studies of synthesized iron oxide nanoparticles using ethanolic extract of Centella asiatica plant

Pravallika, P. L.; Mohan, G. Krishna; Rao, K. Venkateswara; Shanker, Kalakotla

doi:10.1016/j.matlet.2018.10.037

Cited by 49 publications

(12 citation statements)

References 15 publications

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Section: Discussionsupporting

confidence: 93%

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Sari¹

2020

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Background: Lauric acid (LA), a common constituent of coconut oil is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective: The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods: Study was conducted in accordance with the Organization for Economic Cooperation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2000 mg/kg body weight respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15 th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results: Study result showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion: Current finding suggests that single oral administration of LA, even up to 2000 mg/kg body weight did not exhibit any signs of toxicity in SD rats, thus, safe to be used on disease model in animals.

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Section: Discussionsupporting

confidence: 93%

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Sari¹

2020

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“…Furthermore, brain tissues showed normal morphology upon LA treatment, with no alterations to the cell types approaching through normal characteristics based on tissue pattern recognition, including neurons, glia, and microglia cells without any modulation to perivascular region. Thus, our study is supported by previous findings by Pravallika et al (2019) who reported no structural and morphological changes in brain tissues following treatment with ethanolic extract of Centella asiatica plant. Moreover, LA treatment did not alter pancreas and spleen tissue which shows normal architecture, as we know rodent pancreas and spleen are diffused and soft compare to human tissues, which is in line with the previously published study by Prabu, Panchapakesan & Raj (2013) that reveals no morphological variation in tissues of control and hydroalcoholic extract of polyherbal treatment groups.…”

Section: Discussionsupporting

confidence: 92%

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

Khan

Aamir

Sisinthy

et al. 2020

PeerJ

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Background Lauric acid (LA), a common constituent of coconut oil, is used as food additives and supplements in various formulations. Despite various potential pharmacological properties, no scientific evidence on its dose-related toxicity and safety is available till date. Objective The current study was conducted to evaluate acute oral toxicity of LA on normal rats. Methods The study was conducted in accordance with the Organization for Economic Co-operation and Development guidelines (OECD 423) with slight modifications. LA was administered orally to female Sprague Dawley (SD) rats (n = 6/group) at a single dose of 300 and 2,000 mg/kg body weight, respectively, while normal control received vehicle only. Animals from all the three groups were monitored for any behavioural and toxicological changes and mortality for two weeks. Food and fluid consumption, body weight was monitored on daily basis. At the end (on day 15th) of the experimental period, blood was collected for haematological and biochemical analysis. Further, all the animals were euthanized, and internal organs were harvested for histopathological investigation using four different stainings; haematoxylin and eosin, Masson trichrome, Periodic Acid Schiff and Picro Sirius Red for gross pathology through microscopical observation. Results The study results showed no LA treatment-related mortality and morbidity at two different dosages. Daily food and water consumption, body weight, relative organ weight, haematological, and biochemical analysis were observed to be normal with no severe alterations to the internal tissues. Conclusion The current finding suggests that single oral administration of LA, even up to 2,000 mg/kg body weight, did not exhibit any signs of toxicity in SD rats; thus, it was safe to be used on disease models in animals.

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“…Lakshmi Pravallika et al, 2019 synthesized iron oxide nanoparticles using ethanolic extract of Centella asiatica (CAIONPs) by reducing ferrous and ferric chlorides which were administered to Swiss albino mice with a dosage of 2000 mg/kg body weight. Nil effects of the NPs on various tissues were revealed by histopathological studies, indicating that green synthesized NPs were safe for use in biomedical and drug delivery systems 17 . In a similar study by Izadiyan et al, 2018, iron oxide nanoparticles were synthesized using Juglans regia green husk extract by co-precipitation method of FeCl 3 and FeCl 2 and the cytotoxicity tests were performed on mouse embryonic fibroblast cell lines and human colorectal adenocarcinoma cell lines by MTT assay which had no toxic effect on both normal and cancerous cell lines 12 .…”

Section: Introductionmentioning

confidence: 99%

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

Lakshminarayanan

Shereen

Niraimathi

et al. 2021

Sci Rep

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The green synthesis of NPs through plant extracts can be a modest, one-pot alternative synthesis to the conventional physical or chemical method. The prime focus of this study is to produce MNPs by the reducing effect of Bauhinia tomentosa leaf extract, and it was immobilized in porcine pancreatic lipase (PPL). Synthesized NPs were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Raman spectroscopy, UV–Vis Spectrometry, Thermogravimetry, and Differential Scanning Calorimeter (DSC), Zeta potential test, VSM, BET and Fourier Transform Infrared Spectroscopy (FTIR). The effect of process parameters was studied, about the efficiency of immobilization are enzyme stability, the extent of enzyme reusability, its separation from products, the activity of immobilized enzyme, recovery, and its loss. Finally, the immobilized lipase was used for the synthesis of 1,3-diolein using enzyme-mediated esterification of oleic acid and glycerol. Under optimized condition (reaction temp-55 $$^\circ $$ ∘ C; molar ratio-2.5:1; pH-7) diolein yield was achieved to be 94%. Therefore, this work was further used for the industrial production of 1,3-diacylglycerol since a perfect enzyme-catalyzed process was observed.

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Biosynthesis, characterization and acute oral toxicity studies of synthesized iron oxide nanoparticles using ethanolic extract of Centella asiatica plant

Cited by 49 publications

References 15 publications

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Peer Review #1 of "Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats (v0.1)"

Food additive “lauric acid” possess non-toxic profile on biochemical, haematological and histopathological studies in female Sprague Dawley (SD) rats

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

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