Biosynthesis and microscopic study of metallic nanoparticles

Quester, Katrin; Avalos-Borja, M.; Castro-Longoria, Ernestina

doi:10.1016/j.micron.2013.07.003

Cited by 108 publications

(64 citation statements)

References 195 publications

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“…It was seen that through the enzymatic synthesis process, the formation of quasi-spherical NPs was most common, but the morphology of NP depends on the metal ion solution, concentration of biomolecule produced by the microbes, incubation time, and incubation condition (Quester et al 2013). Several other members from same fungal family were also reported for the Ag-NPs formation viz.…”

Section: Transmission Electron Microscopic Analysismentioning

confidence: 99%

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Barapatre

Aadil

Jha

2016

Bioresour. Bioprocess.

138

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Background:The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO 3 using two lignin-degrading fungus Aspergillus flavus (AfAg-NPs) and Emericella nidulans (EnAg-NPs). The prepared Ag-NPs were characterized by different spectroscopic techniques. Antibacterial activity of prepared Ag-NPs was demonstrated against selected Gram negative (Escherichia coli and Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria in the term of minimum bactericidal concentration (MBC) and susceptibility constant (Z). The synergistic antibacterial activity of Ag-NPs with four conventional antibiotics was also determined by the fractional inhibitory concentration index (FICI) using the checkerboard microdilution method. The antibiofilm potential of Ag-NPs was also tested. Results:The plasmon surface resonance of biosynthesized Ag-NPs shows its characteristic peaks at UV and visible region (~450 and 280 nm). Fourier transform infrared spectrometer (FTIR) analysis confirms the nature of the capping agents as protein (enzyme) and indicates the role of protein (enzyme) in reduction of silver ions. The average particle size and charge of synthesized Ag-NPs was ~100 nm and ~−20 mV, respectively. X-ray diffraction (XRD) and TEM analysis confirmed the purity, shape, and size (quasi-spherical, hexagonal, and triangular) of Ag-NPs. Energy-dispersive X-ray spectroscopy (EDX) data validate the biological synthesis of Ag-NPs. Low MBC and high susceptibility constant indicate the high antimicrobial strength of biosynthesized Ag-NPs. The antibacterial analysis demonstrates the synergistic antimicrobial activity of Ag-NPs with antibiotics. This study also shows that biosynthesized Ag-NPs have ability to inhibit the biofilm formation by 80-90 %. Conclusion:The Aspergillus flavus and Emericella nidulans-mediated biosynthesized Ag-NPs have significant antimicrobial activity and demonstrate synergistic effect in combination with antibiotics. It suggests that nanoparticles can be effectively used in combination with antibiotics to improve the efficacy of antibiotics against pathogenic microbes. The substantial antibiofilm efficiency of biosynthesized Ag-NPs would also be helpful against sensitive and multidrug-resistant strains.

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Section: Transmission Electron Microscopic Analysismentioning

confidence: 99%

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Barapatre

Aadil

Jha

2016

Bioresour. Bioprocess.

138

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“…New methods [28,34] [5,35]. All the involved parameters should be considered for an optimization and control of the shape and size of nanoparticles and the properties derived from them.…”

Section: Physicochemical Characterization Of Agnpsmentioning

confidence: 99%

“…Following this idea a number of methods had been reported in which nanomaterials have been obtained using different biocompatible organics [5]. However, most of the green methods propose the use of biological extracts or organisms, including plants, algae, fungi, bacteria and even viruses [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…However, most of the green methods propose the use of biological extracts or organisms, including plants, algae, fungi, bacteria and even viruses [5][6][7][8]. These methods include the intracellular or extracellular production of nanoparticles of different elements, mainly metals, oxides or sulphides and noble metal alloys by a variety of microorganisms [7] and plants extracts [6].…”

Section: Introductionmentioning

confidence: 99%

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Four psychrophilic bacteria from Antarctica extracellularly biosynthesize at low temperature highly stable silver nanoparticles with outstanding antimicrobial activity

Javani

Marı́n

Amils

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Brassica juncea (Indian mustard) was the second species in which the synthesis of MeNPs was studied [13,14]. Besides alfalfa and Indian mustard, some other plant species have been tested for the capacity to synthesize MeNPs [6,15]. …”

Section: Introductionmentioning

confidence: 99%

In vivo synthesis of nanomaterials in plants: location of silver nanoparticles and plant metabolism

Marchiol

Poščić

Giordano³

et al. 2014

Nanoscale Res Lett

106

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Metallic nanoparticles (MeNPs) can be formed in living plants by reduction of the metal ions absorbed as soluble salts. It is very likely that plant metabolism has an important role in MeNP biosynthesis. The in vivo formation of silver nanoparticles (AgNPs) was observed in Brassica juncea, Festuca rubra and Medicago sativa. Plants were grown in Hoagland's solution for 30 days and then exposed for 24 h to a solution of 1,000 ppm AgNO3. In the leaf extracts of control plants, the concentrations of glucose, fructose, ascorbic acid, citric acid and total polyphenols were determined. Total Ag content in plant fractions was determined by inductively coupled plasma atomic emission spectroscopy. Despite the short exposure time, the Ag uptake and translocation to plant leaves was very high, reaching 6,156 and 2,459 mg kg−1 in B. juncea and F. rubra, respectively. Ultrastructural analysis was performed by transmission electron microscopy (TEM), and AgNPs were detected by TEM X-ray microanalysis. TEM images of plant fractions showed the in vivo formation of AgNPs in the roots, stems and leaves of the plants. In the roots, AgNPs were present in the cortical parenchymal cells, on the cell wall of the xylem vessels and in regions corresponding to the pits. In leaf tissues, AgNPs of different sizes and shapes were located close to the cell wall, as well as in the cytoplasm and within chloroplasts. AgNPs were not observed in the phloem of the three plant species. This is the first report of AgNP synthesis in living plants of F. rubra. The contents of reducing sugars and antioxidant compounds, proposed as being involved in the biosynthesis of AgNPs, were quite different between the species, thus suggesting that it is unlikely that a single substance is responsible for this process.MSC 201092 Biology and other natural sciences; 92Cxx Physiological, cellular and medical topics; 92C80 Plant biology

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Biosynthesis and microscopic study of metallic nanoparticles

Cited by 108 publications

References 195 publications

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

Four psychrophilic bacteria from Antarctica extracellularly biosynthesize at low temperature highly stable silver nanoparticles with outstanding antimicrobial activity

In vivo synthesis of nanomaterials in plants: location of silver nanoparticles and plant metabolism

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