Biosynthesis and display of diverse metal nanoparticles by recombinant Escherichia coli

Abstract

This study used the biomolecule, eumelanin, as an agent for the reduction of metal ions. Our results demonstrate the effectiveness of synthesizing diverse metal nanoparticles through the use of recombinant E. coli expressing Rhizobium 10 65 Fig. 1 EuMel from R. etli mediates the synthesis of gold nanoparticles. (A) Photograph of solutions in the presence or absence of 1 mM L-DOPA and Au 3+ , respectively. (B) TEM image of gold nanoparticles synthesized by EuMel. Scale bar: 20 nm. (C) TEM images of R. etli cells. Cells were incubated with or without 1 mM L-DOPA for 12 h. Subsequently, 2mM 70 of Au 3+ or water was added. Scale bar: 200 nm.

Biosynthesis of Metal Nanoparticles: Novel Efficient Heterogeneous Nanocatalysts

“…In this way, the production of metal nanoparticles by fungi is one of the most successful strategies [ 42 , 50 , 51 , 52 , 53 ].…”

“…The TEM analysis demonstrated that the AuNPs showed a particle size average of around 12 nm ( Figure 10 D). The strategy was successfully applied to other metals obtaining NPs with a particle size between 7 and 13 nm [ 53 ].…”

“…( A ) TEM image of R. etli cell in the presence of 3-(3,4-dihydroxyphenyl)- l -alanine ( l -DOPA) and Au ions; ( B ) TEM image of R. etli cell in the presence of Au ions; ( C ) TEM image of R. etli cell in the presence of l -DOPA; ( D ) TEM of the synthesized AuNPs. Reproduced with permission from [ 53 ]. Copyright the Royal Society of Chemistry, 2014…”

Electron-Transport-Chain-Mediated Selective Growth of Gold Nanocrystals in the Intermembrane Space of Live Microbial Cells

“…In the previous reports, GNCs were synthesized in vitro by using cellular extracts including reductases . Alternatively, GNCs were formed randomly inside the cells by intracellular reductive proteins such as metallothionein and phytochelation synthase expressed in recombinant microbial cells. , Similarly, GNCs were synthesized on the cell surface by reductive enzymes in extracellular polymeric substances, which play a role in microbial activity to protect cells from environmental stresses such as high salinity and extreme pH conditions, surrounding microbial cells …”