Biostimulation with the ram effect increases the follicle recruitment, ovulatory diameter, and embryo viability rate in superovulated ewes

Taira, Akihiro; Brandão, Felipe Zandonadi; Brair, Viviane Lopes; Cosentino, Isabel Oliveira; Leal, Felipe Seabra Cardoso; Ribeiro, Ana Clara Sarzedas; Balaro, M. F. A.; Batista, Ríbrio Ivan Tavares Pereira; Souza-Fabjan, Joanna Maria Gonçalves; Fonseca, J. F. da; Ungerfeld, Rodolfo

doi:10.1016/j.theriogenology.2022.01.021

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Briefly, it has been concluded that the use of exogenous melatonin prior to superovulation during the breeding season reduces the yield and the quality of the embryos. At the same time, these values are low in both groups when compared to other studies conducted during the breeding season (Alvarez et al., 2014; Cordeiro et al., 2003; Figueira et al., 2020; Forcada et al., 2011; Lima et al., 2015; Maciel et al., 2019; Mayorga et al., 2011; Menchaca, Vilariño, Pinczak, et al., 2009; Merai et al., 2017; Taira et al., 2022). As mentioned above, this difference may be due to the influence of the intrafollicular hormone mechanism.…”

Section: Discussionmentioning

confidence: 51%

The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes

Yesilkaya,

Erdem

2024

Reprod Domestic Animals

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The aim of this study was to determine the effect of exogenous melatonin administration on transferable embryos by increasing total antioxidant status before superovulation in Assaf ewes. Selected ewes were randomly divided into two equal groups: melatonin (n = 9) and control (n = 9). In the melatonin group, a melatonin implant (18 mg melatonin, Regulin®, Ceva, Turkey) was placed under the skin of the ear 7 days prior to insertion of the progesterone‐containing sponge. In the control group, a physiological saline solution was injected under the skin of the ear on the same day. The same superovulation protocol was used in both groups. In addition, blood samples for determination of Glutathione peroxidase, superoxide dismutase, total antioxidant status and total oxidant status concentrations were collected on five different days, including the day of melatonin implant placement (Day−7), vaginal sponge insertion (Day 0), vaginal sponge removal (Day 11), mating (Day 12–13) and uterine flushing (Day 19). Embryos were collected by laparotomy on the 7th day after mating. Uterine flushing taken into petri dishes were scanned under a stereomicroscope, and the quality and developmental stages of the embryos were recorded. In the study, total corpus luteum count and total cell count were found to be higher in the control group than in the melatonin group (p < .05). When the results were evaluated in terms of oxidative stress index, a negative correlation was found between the total number of corpus luteum, number of cells obtained, count of transferable embryos and number of Grade 1 embryos on Day 0. There was also a positive correlation oxidative stress index and the number of unfertilized oocytes on Day−7. As a result, exogenous melatonin administration prior to superovulation during the breeding season is thought to have a negative effect on embryo yield and quality. Therefore, the use of exogenous melatonin in MOET studies during the breeding season is recommended to be investigated in new studies.

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Section: Discussionmentioning

confidence: 51%

The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes

Yesilkaya,

Erdem

2024

Reprod Domestic Animals

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“…In some cases, AI is performed at natural estrus that uses light treatments to synchronize the estrus of ewes [ 9 , 10 ]. In addition, in the sexual season or estrus, the so-called male or ram effect can synchronize estrus [ 3 , 11 , 12 ]. In general, AI at natural estrus is more successful if conducted in the reproductive season rather than in the anestrus period [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Artificial Insemination at Natural Estrus in Organic Churra Ewes

Palacios

Abecia

Plaza

et al. 2022

Veterinary Sciences

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Hormonal treatments used in the artificial insemination (AI) of sheep can cause several physiological problems that can affect negatively fertility and animal health; however, AI protocols based on the detection of natural estrus offer a more sustainable option and can achieve high fertility. In this study, an AI protocol at natural estrus in organic Churra sheep was performed. In the first phase (AI protocol development), 125 ewes were exocervically inseminated, and their fertility was assessed based on the following factors: number of AI, physiological state, body condition, estrus detection–AI interval, and vaginal fluids in cervix. That protocol was repeated for six consecutive years. In all individuals, fertilities based on the timing of insemination after estrus detection were very high. Lactating ewes produced better results than did dry ewes, which was probably because of the better feeding of the former. In addition, double insemination increased the fertility of ewes whose estrus was detected within 16 h of onset. Body condition and amount of vaginal fluid were correlated with fertility. Exocervical inseminations at natural estrus can produce acceptable fertility and prolificity in Churra ewes.

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The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes

Ribeiro,

Pinto,

Santos

et al. 2024

Animal Reproduction Science

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Biostimulation with the ram effect increases the follicle recruitment, ovulatory diameter, and embryo viability rate in superovulated ewes

Cited by 6 publications

References 27 publications

The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes

The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes

Efficiency of Artificial Insemination at Natural Estrus in Organic Churra Ewes

The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes

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