Biosensor based on inhibition of monoamine oxidases A and B for detection of β-carbolines

Radulescu, Maria-Cristina; Bucur, Madalina-Petruta; Bucur, Bogdan; Radu, Gabriel Lucian

doi:10.1016/j.talanta.2015.02.013

Cited by 13 publications

(6 citation statements)

References 30 publications

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“…Two biosensors were used for each sample: (i) one based on MAO-A in order to detect the presence of all β-carbolines and (ii) the other based on MAO-B to identify the presence of norharmane in mixtures. The analysis of food samples with the MAOs biosensors provide the results as both content of norharmane from MAO-B biosensor and an evaluation of the overall content of β-carbolines expressed as equivalent norharmane quantity that produce a similar inhibition [ 34 ]. The accuracy of the MAO-A and MAO-B biosensors was verified by the analysis of norharmane-spiked samples of unroasted green coffee and smoked fish, for which recoveries of 99.0–110.1% were calculated.…”

Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

“…HRP is also known to intermediate the transfer of electrons between electrode and H 2 O 2 for a desired improved sensitivity. Nevertheless, the peroxidase usage is not strictly required since there are: (i) very effective electrochemical mediators for H 2 O 2 such as copper stabilized Prussian blue [ 34 ] so efficient that are considered to be “artificial peroxidases” [ 57 ], (ii) the possibility to mediate the electrons directly to the some oxidases such as glucose oxidase that can accommodate a variety of oxidants as co-substrates unlike other oxidases such as alcohol oxidase which is a ‘true oxidase’ i.e., selective to dioxygen as the sole acceptable cosubstrate [ 58 ] or (iii) even the possibility of direct electron transfer between the electrode and the oxidases [ 59 ].…”

Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

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Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Bucur

Purcarea²,

Andreescu

et al. 2021

Sensors

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Enzymatic biosensors enjoy commercial success and are the subject of continued research efforts to widen their range of practical application. For these biosensors to reach their full potential, their selectivity challenges need to be addressed by comprehensive, solid approaches. This review discusses the status of enzymatic biosensors in achieving accurate and selective measurements via direct biocatalytic and inhibition-based detection, with a focus on electrochemical enzyme biosensors. Examples of practical solutions for tackling the activity and selectivity problems and preventing interferences from co-existing electroactive compounds in the samples are provided such as the use of permselective membranes, sentinel sensors and coupled multi-enzyme systems. The effect of activators, inhibitors or enzymatic substrates are also addressed by coupled enzymatic reactions and multi-sensor arrays combined with data interpretation via chemometrics. In addition to these more traditional approaches, the review discusses some ingenious recent approaches, detailing also on possible solutions involving the use of nanomaterials to ensuring the biosensors’ selectivity. Overall, the examples presented illustrate the various tools available when developing enzyme biosensors for new applications and stress the necessity to more comprehensively investigate their selectivity and validate the biosensors versus standard analytical methods.

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Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Bucur

Purcarea²,

Andreescu

et al. 2021

Sensors

Self Cite

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“…Since then, many derivatives of monoamine oxidase inhibitors were developed, with the ability to inhibit effectively, selectively and reversibly the enzymatic activity of MAO [ 78 ]. The most conventional technique used in drug control is chromatography [ 79 ]. However, this technique could be laborious and time consuming in contrary of biosensors that could be rapid, low cost and provide the required sensitivity needed in quantitative analysis of drugs.…”

Section: Application Of Electrochemical Biosensors Based On Enzymementioning

confidence: 99%

Recent Advances in Electrochemical Biosensors Based on Enzyme Inhibition for Clinical and Pharmaceutical Applications

Harrad

Bourais

Mohammadi

et al. 2018

Sensors

110

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A large number of enzyme inhibitors are used as drugs to treat several diseases such as gout, diabetes, AIDS, depression, Parkinson’s and Alzheimer’s diseases. Electrochemical biosensors based on enzyme inhibition are useful devices for an easy, fast and environment friendly monitoring of inhibitors like drugs. In the last decades, electrochemical biosensors have shown great potentials in the detection of different drugs like neostigmine, ketoconazole, donepezil, allopurinol and many others. They attracted increasing attention due to the advantage of being high sensitive and accurate analytical tools, able to reach low detection limits and the possibility to be performed on real samples. This review will spotlight the research conducted in the past 10 years (2007–2017) on inhibition based enzymatic electrochemical biosensors for the analysis of different drugs. New assays based on novel bio-devices will be debated. Moreover, the exploration of the recent graphical approach in diagnosis of reversible and irreversible inhibition mechanism will be discussed. The accurate and the fast diagnosis of inhibition type will help researchers in further drug design improvements and the identification of new molecules that will serve as new enzyme targets.

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“…The referred article described a fast analysis method for β-carbolines based on the inhibition of MAO [79]. The MAO-A is inhibited by all three tested β-carbolines (harmane, norharmane and harmaline), while MAO-B is inhibited only by norharmane.…”

Section: Other Inhibition Systemsmentioning

confidence: 99%

Bioelectronic Tongues Employing Electrochemical Biosensors

Valle

2016

Bioanalytical Reviews

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This chapter presents recent advances concerning work with electronic tongues that employ electrochemical biosensors, that is, bioelectronic tongues. (Bio)electronic tongues represent a new methodological use of (bio)sensors; they start by the use of biosensor arrays and assume the coupling of the obtained complex response with advanced chemometric data treatment; the goal is improving performance of existing sensors. Most of the bioelectronic tongues reported employ enzyme biosensors, essentially based on potentiometric or voltammetric/ amperometric transduction. This report is organized considering the different forms to incorporate biosensors, i.e. considering the number of biosensors in the array, the number of different enzymes used, if the determination is aimed to substrates or inhibitors, etc. Significant applications in real problem-solving, mainly in the food and clinical or environmental fields, are commented.

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Biosensor based on inhibition of monoamine oxidases A and B for detection of β-carbolines

Cited by 13 publications

References 30 publications

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Recent Advances in Electrochemical Biosensors Based on Enzyme Inhibition for Clinical and Pharmaceutical Applications

Bioelectronic Tongues Employing Electrochemical Biosensors

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