Fluorescence resonance energy transfer (FRET) is recognized as a sensitive and reliable analytical technique and has been widely used in biological assays. To obtain improved FRET efficiency and analytical performance, it is of continuing interest to search for new energy donor-acceptor pairs. In the past few years, the use of anti-Stokes fluorophores including upconverting phosphors (UCP) and multiphotonexcited dyes as energy donors which can be excited in the near-infrared (NIR) region has successfully c… Show more
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