Biosensing of microcystins in water samples; recent advances

Kordasht, Houman Kholafazad; Hassanpour, Soodabeh; Baradaran, Behzad; Nosrati, Rahim; Hashemzaei, Mahmoud; Mokhtarzadeh, Ahad; Guárdia, Miguel de la

doi:10.1016/j.bios.2020.112403

Cited by 48 publications

(22 citation statements)

References 115 publications

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“…5,[32][33][34] Among the identified over 100 structural analogues of MCs, MC-LR is one of the most abundant and toxic congeners. 35 MC-LR was classified by the International Agency for Research on Cancer (IARC) as a possible human carcinogen (Group 2B). 36 In our present study, much higher levels of MC-LR were observed in pond-ditch water than in tap water.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

Wang¹,

Huang²,

Huang³

et al. 2021

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Background: Protein phosphatase 2A (PP2A, a serine/threonine phosphatase) is frequently inactivated in many types of cancer, including primary liver cancer (PLC). Genetic variations in PP2A subunits have been reported to be associated with the risk of many types of cancer but rarely in PLC. This study aims to assess the association between functional polymorphisms of PP2A subunit genes and the risk of PLC in Chinese. Methods: In a case-control study with a total of 541 PLC patients and 547 controls in Guangxi province of Southern China, we genotyped six putatively functional polymorphisms (rs10421191G>A, rs11453459del>insG, rs1560092T>G, rs7840855C>T, rs1255722G>A and rs10151527A>C) of three PP2A subunit genes (PPP2R1A, PPP2R2A and PPP2R5E) using matrixassisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry platform. Results: The rs11453459insG variant genotypes (ins/ins+del/ins) of PPP2R1A were found to be significantly associated with an increased risk of PLC compared with the del/del genotype (adjusted OR = 1.290, 95% CI = 1.009-1.650), and the number of insert G allele worked in a dose-dependent manner (P trend = 0.007). The stratified analysis showed that the effects of rs11453459insG variant genotypes were more evident in the subgroup who drink pond-ditch water (adjusted OR = 3.051, 95% CI = 1.264-7.364) than those never drink (P = 0.041). The carriers of rs11453459 del/ins genotype had a significantly lower level of PPP2R1A mRNA expression in liver cancer tissues than those of the del/del genotype (P = 0.021). Furthermore, we used microcystin-LR, a carcinogen presents in the pond-ditch water, to treat human peripheral blood mononuclear cells and found that the cells from carriers of rs11453459insG variant genotypes induced more DNA oxidative damages than those from the del/del genotype carriers (P < 0.001). Conclusion: These findings suggest that the PPP2R1A rs11453459del>insG polymorphism is associated with an increased risk of PLC, especially for persons with a history of drinking pond-ditch water. This insertion/deletion polymorphism may be a susceptible biomarker for PLC in Chinese.

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Section: Discussionmentioning

confidence: 99%

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

Wang¹,

Huang²,

Huang³

et al. 2021

PGPM

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“…MCs are a group of cyclic heptapeptide molecules produced by cyanobacteria. More than 100 different congeners of MCs have been identified ( Svirčev et al, 2017 ; Kordasht et al, 2020 ). Congeners mainly differ in their amino acids at positions 2 and 4, as indicated in their names.…”

Section: Discussionmentioning

confidence: 99%

“…A single amino acid difference in its molecular structure deems microcystin-LR (MC-LR) as a relatively toxic congener, as its acute or subchronic exposure has been shown to cause health damage, especially hepato-, nephro-, and neuro-toxicity. Microcystin-RR (MC-RR), on the contrary, has decreased toxicity ( Gupta et al, 2003 ; Wu et al, 2017 ; Wang et al, 2018 ; Kordasht et al, 2020 ; Zhao et al, 2020 ). The primary toxicologic mechanism of MCs has been well established and related to the inhibition of the catalytic subunit of protein phosphatases 1 and 2A (PP1 and PP2A) that perform hyperphosphorylation of cellular proteins and are associated with signaling events related to the cytoskeleton and cell metabolism, proliferation, and apoptosis ( Campos and Vasconcelos, 2010 ; Sassolas et al, 2011 ; Liu and Sun, 2015 ; Fontanillo et al, 2016 ; Fontanillo and Köhn, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-Fibrotic Effects of Low Toxic Microcystin-RR on Bleomycin-Induced Pulmonary Fibrosis: A Comparison with Microcystin-LR

et al. 2021

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Idiopathic pulmonary fibrosis (IPF) is a chronic progressive interstitial pulmonary disease characterized with radiographically evident pulmonary infiltrates and extracellular matrix deposition with limited treatment options. We previously described that microcystin-LR (MC-LR) reduces transforming growth factor (TGF)-β1/Smad signaling and ameliorates pulmonary fibrosis in bleomycin (BLM)-induced rat models. In the present study, we further demonstrate that microcystin-RR (MC-RR), an MC congener with lower toxicity than MC-LR, exerted an anti-fibrotic effect on BLM-induced pulmonary fibrosis rodent models and compared it with MC-LR. Our data show that MC-RR treatment attenuated BLM-associated pulmonary inflammation and collagen deposition in both therapeutic and preventive models. MC-RR reduced the expression of fibrotic markers, including vimentin, α-smooth muscle actin, collagen 1α1, and fibronectin, in rat pulmonary tissues. Furthermore, the core features of BLM-induced pulmonary fibrotic lesions were better alleviated by MC-RR than by MC-LR. MC-RR treatment substantially decreased the number of pulmonary M2 macrophages. In vitro, MC-RR attenuated the epithelial-mesenchymal transition and fibroblast-myofibroblast transition triggered by M2 macrophages. Therefore, we highlight MC-RR as a promising molecule for developing therapeutic and prophylactic strategies against IPF, a refractory lung disease.

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“…Eles têm sido usados para a determinação de vários analitos. As reações redox causaram alteração na tensão, potencial e corrente no sistema transdutor (KORDASHT, et al 2020). A maioria das aplicações desses biossensores diz respeito à saúde e ao controle ambiental.…”

Section: Biossensoresunclassified

“…A maioria das aplicações desses biossensores diz respeito à saúde e ao controle ambiental. Diferentes variedades de biossensores ópticos, incluindo fluorescência, absorção, refração, ressonância plasmônica de superfície (SPR), fibra óptica e luminescência (KORDASHT, et al 2020).…”

Section: Biossensoresunclassified

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

Rodrigues

De-Carli

Vicentin

et al. 2021

Bol. Lab. Hidrobiol.

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Muitos corpos hídricos têm sido degradados devido à interferência humana. A poluição causada pelas atividades antrópicas vem comprometendo a qualidade da água e promovendo eventos de eutrofização, afetando toda estrutura trófica do ambiente, podendo assim acarretar na proliferação de cianobactérias tóxicas. Nesse sentido, o conhecimento sobre esses organismos é de grande importância, principalmente em corpos hídricos com usos múltiplos. Esta pesquisa representa uma revisão de trabalhos publicados envolvendo qualidade ambiental e ecolotoxicologia aquática, tendo como foco estudos relacionados às cianobactérias e cianotoxinas. Para o trabalho foram analisados livros textos e artigos publicados entre 2010 e 2020 em periódicos nacionais e internacionais. Na literatura publicada nos últimos anos são apresentadas as principais metodologias envolvidas na detecção e remoção de cianotoxinas, além de bioensaios ecotoxicológicos. Fica evidente a importância do tratamento de efluentes e controle das fontes de poluição difusas e pontuais no ecossistema promovendo a melhoria da qualidade da água e controle de cianobactérias.ABSTRACTCyanobacteria play an important role among producers in aquatic ecosystems, however when associated with eutrophication they become an ecological and public health problem. The problem involving cyanobacteria is the blooming of potentially toxic strains. The present work aims to present and discuss the problems related to the proliferation of cyanobacteria in limnological environments, and its consequences for dynamics in the environment and effects on environmental health. In this sense, knowledge about these organisms is of great importance, especially in water bodies with multiple uses. This research represents a review of published works involving environmental quality and aquatic ecotoxicology, focusing on studies related to cyanobacteria and cyanotoxins. For the present work, textbooks and articles published between 2010 and 2020 in national and internationaljournals were analyzed. In the literature published in recent years, the main methodologies involved in the detection and removal of cyanotoxins are presented, in addition to ecotoxicological bioassays. The study of cyanobacteria and their metabolites comprises an area of interest that reaches a greater dimension when impacts on the quality of water supply are evident, thus the importance of treating effluents and controlling pollution sources in the ecosystem is evident, promoting improvement and maintenance the quality of the water bodies.

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Biosensing of microcystins in water samples; recent advances

Cited by 48 publications

References 115 publications

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

Genetic Variant of PP2A Subunit Gene Confers an Increased Risk of Primary Liver Cancer in Chinese

Anti-Fibrotic Effects of Low Toxic Microcystin-RR on Bleomycin-Induced Pulmonary Fibrosis: A Comparison with Microcystin-LR

CIANOBACTÉRIAS E CIANOTOXINAS EM AMBIENTES AQUÁTICOS: uma breve revisão sobre os principais métodos detecção, remoção e impactos sobre a saúde humana = CYANBACTERIA AND CYANOTOXINS IN AQUATIC ENVIRONMENTS: a short review on the main methods of detection, removal and impacts on human health

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