Bioremediation of Spent Engine Oil Contaminated Soil by Using Fungus, <i>Penicillium sp.</i>

Abstract

Abstract. This study investigated the ability of Penicillium sp. to bio-remediate spent engine oil contaminated soil both in vitro and in vivo. In the in vitro assay, mycelium of a seven day old culture of Penicillium sp. grown on Sabouraud Dextrose Agar (SDA) was punched out using a 0.5mm Cork borer and inoculated on the centre of Petri dishes containing the spent and unspent engine oil and incubated for seven days and daily reading of the mycelia growth obtained using a metre rule. For the in vivo assay, soil received 0 (control), 20/180, 40/360, 60/540, 80/720 and 100ml/900mm concentrations/treatments (inoculation with mycelium of Penicillium sp.). Seeds of Telfeira occidentalis was sown on the soil and assessed for growth performance (plant height, leaf area (using a metre rule) and leaf count (number of leaves) for 7, 14, 21 and 28 Days after Planting (DAP). Results of the in vitro assay showed a significant increase (p<0.05) in the growth diameter of Penicillium sp. relative to control. Results of the in vivo assay showed that spent engine oil had no significant effect (p<0.05) on the growth performance of T. occidentalis at 7, 14, 21 and 28 DAP and on fresh and dry weight (g) 28 DAP relative to control. After 28 days of plant growth, the added spent engine oil was no longer detected. The plant began producing pods 61 DAP. This study showed that Penicillium sp. can biodegrade hydrocarbons present in spent engine oil and as such is a good tool for bioremediation.

Microbiological Diversity and Biotechnological Potential of the Soil Ecosystem of a High-Mountainous Landfill

“…Many researchers confirmed that the diversity of the fungal community is dominant among other inhabitants in the oil contamination of soils. Apparently, Aspergillus and Penicillium are the most common species in high oil-contaminated soils [53][54][55][56]. The study of actinomycetes showed that this group did not contain a large species diversity, and the group was mainly represented by the Cinereus section of the genus Streptomyces, in a minor amount.…”

Optimization of Fruit Garbage Enzymes Requirements for Biocatalytic Remediation of Used Motor Oil-Contaminated Soil

“…It was also reported that the increase in TOC of oil-polluted soil was observed to correspond with the increase in O&G content. 26,51,52) On the aspect of nitrogen contents, it was observed that the nitrogen content of soil increased with increase in oil contamination levels. The nitrogen content of the control soil was 2.4 mg/kg (0.24%), which was lower relative to the contaminated soils whose values were 3.6 mg/kg (0.36%) and 3.8 mg/kg (0.38%) for 5 and 10% oil pollution levels, respectively.…”

Hydrocarbon Degrading Potentials and Antibiotic Susceptibility Test of Bacteria Isolated from Amended Soil in Ilorin

“…Contaminants build up in the soil, are absorbed by plant roots, seep into surface and ground water, and disperse as aerosolized pollutants into the air, all of which are harmful to human and animal health (Ghaemi et al, 2015;Edyta and Dariusz, 2020). Contaminants such as petroleum hydrocarbons and heavy metals are extremely harmful to the environment, and human exposure to these dangerous substances can have serious health repercussions as well as change the socioeconomic activities of a community (Umana et al, 2016;Aniefiok and Udo, 2019).…”