Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

Strong, Peter James; Burgess, J.E.

doi:10.2166/wst.2007.487

Cited by 31 publications

(18 citation statements)

References 22 publications

(27 reference statements)

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“…The concentration of phenolic compounds increased slightly by the end of the fungal treatment, which indicated that a phenolic compound was synthesised during the fungal treatment. This had also occurred with the treatment of distillery wastewaters (Strong & Burgess, 2007). Eggert et al (1996) identified a tryp-tophan-derived metabolite (3-hydroxyanthranilate) that was secreted by Pycnoporus cinnabarinus, which functioned as a mediator and allowed laccase to oxidise substances with higher oxidation potentials.…”

Section: Degradation Of Phenolic Compoundsmentioning

confidence: 97%

“…7). The treatment of a brandy distillery wastewater had previously yielded laccase concentrations of 4 644 ± 228 units/L in shakeflask cultures (Strong & Burgess, 2007).…”

Section: Laccase Synthesismentioning

confidence: 99%

“…The strain of fungus used in this study (Trametes pubescens MB 89) has previously demonstrated better potential for laccase production and bioremediation than three other fungi when treating wine-distillery wastewater (Strong & Burgess, 2007). Laccase production during fungal remediation was also assessed, as the wastewater could be utilised as a potential medium in which to produce the enzyme.…”

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confidence: 99%

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Fungal Remediation and Subsequent Methanogenic Digestion of Sixteen Winery Wastewaters

Strong

2016

SAJEV

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Sixteen winery wastewaters were partially characterised during peak wine production. Raw wastewaters were inoculated with Trametes pubescens MB 89 to establish whether a submerged culture could be used to treat winery wastewaters, and whether fungally-treated wastewater would be rendered more degradable by secondary biological treatment using methanogenic bacteria. Additionally, laccase activity was monitored during the treatment to determine if phenolic compounds present in the wastewater would stimulate the synthesis of the fungal enzyme. Fungal treatment using T. pubescens MB 89 lowered the chemical oxygen demand (COD) and increased the acidic pH values of all wastewater samples. Five of the wastewater samples showed an increase in laccase synthesis, but the concentrations were low and did not relate to any individual characteristic that was assayed for. It proved advantageous to methanogenic digestion to fungally pretreat samples that had higher initial phenolic compound and colour concentrations, but disadvantageous for wastewaters with low initial phenolic compound and colour concentrations. Anaerobic digestion of fungally-treated and raw samples generally showed little difference with regard to total COD removal and final pH. The incorporation of white-rot fungus into a pretreatment process such as a jet loop reactor or rotating biological disc contactor could prove to be highly advantageous to cellar wastewater treatment.

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Section: Degradation Of Phenolic Compoundsmentioning

confidence: 97%

“…7). The treatment of a brandy distillery wastewater had previously yielded laccase concentrations of 4 644 ± 228 units/L in shakeflask cultures (Strong & Burgess, 2007).…”

Section: Laccase Synthesismentioning

confidence: 99%

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confidence: 99%

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Fungal Remediation and Subsequent Methanogenic Digestion of Sixteen Winery Wastewaters

Strong

2016

SAJEV

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“…Fungi are capable of degrading these compounds or using them as a carbon and energy source. Fungal digestion of wine distillery wastewaters using Trametes pubescens MB 89 has been shown to remove a large portion of the total phenols, COD, colour, increase the pH and produce a high concentration of laccase (Strong and Burgess 2007). Laccase, one of the major fungal enzymes, is a phenoloxidase that can be obtained in high concentrations from fungal cultures.…”

Section: Introductionmentioning

confidence: 99%

Fungal remediation of Amarula distillery wastewater

Strong

2009

World J Microbiol Biotechnol

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Amarula Cream is an alcoholic beverage derived from the distillation of fermented marula fruit and to date there is no scientific data as to the characteristics of the distillery wastewater generated from its production. The wastewater was found to have a chemical oxygen demand (COD) of 27 g/l, a pH of 3.8, a high concentration of phenolic compounds (866 mg/l) and a high suspended solids content (10.5 g/l), all of which could adversely affect biological treatment. Full-strength wastewater was treated using shake-flask monocultures of four white rot fungi (Trametes pubescens MB 89, Ceriporiopsis subvermispora, Pycnoporus cinnabarinus or Phanerochaete chrysosporium) at pH 5.0 with no additional carbon or nitrogen supplements. Trametes pubescens performed the best with regards to degrading phenolic compounds, COD and colour, while P. cinnabarinus improved the pH to the greatest extent. Laccase synthesis was only detected in the T. pubescens and C. subvermispora cultures. Six wastewater concentrations (100, 80, 60, 40, 20 and 10%) were assessed at pH 4.5 to establish an optimum concentration for remediation and laccase production by T. pubescens. Similar COD removal efficiencies (71-77%) and phenolic removal efficiencies (87-92%) were achieved at all concentrations. The phenolic removal efficiencies improved by approximately 5% compared to the screening experiment at pH 5.0, indicating that the laccase was more efficient at pH 4.5. The pH became more basic as a result of treatment and the colour decreased for samples below 60% wastewater concentration. The maximum laccase activity (1063 ± 26 units/l) was obtained in the 80% wastewater concentration. This study has resulted in the first characterization of Amarula distillery wastewater and showed that it has a high phenolic compound concentration, COD and suspended solids content. It was possible to biologically treat the wastewater at full strength using a number of white-rot fungi just by raising the pH.

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“…This group of microorganisms is capable of completely degrading polymers of phenolic origin, including lignin. The biodegradation activity toward some wide spread phenolic pollutants is studied together with the laccase production and expression of phenol oxidase activities in general [4,20]. Lignin degradation by white-rot fungi is known as a cometabolic process, and growth of white-rot fungi on monoaromatics (constituents of the lignin macromolecule) have only rarely been described [3].…”

Section: Introductionmentioning

confidence: 99%

Growth of Trametes versicolor on phenol

Yemendzhiev

Gerginova

Krastanov

et al. 2008

J Ind Microbiol Biotechnol

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Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phenol in 6 days. The dynamics of the phenol degradation process was investigated. The process was characterized by specific growth rate micromax 0.33 h(-1), metabolic coefficient k=4.4, yield coefficient Yx/s=0.23 and rate of degradation Q=0.506 h(-1). The intracellular activities of phenol hydroxylase (0.333 U/mg protein) and cis,cis-muconate lactonizing enzyme (0.41 U/mg protein) were demonstrated for the first time in this fungus. In an attempt to estimate the occurrence of gene sequences in T. versicolor 1 related to phenol degradation pathway a dot blot analysis with total DNA isolated from this strain was performed. Two synthetic oligonucleotides were used as hybridizing probes. One of the probes was homologous to the 5'end of phyA gene coding for phenol hydroxylase in Trichosporon cutaneum ATCC 46490. The other probe was created on the basis of cis,cis-muconate lactonizing enzyme coding gene in T. cutaneum ATCC 58094. The results of these investigations showed that T. versicolor 1 may carry genes similar to those of Trichosporon cutaneum capable to degrade phenol.

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Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

Cited by 31 publications

References 22 publications

Fungal Remediation and Subsequent Methanogenic Digestion of Sixteen Winery Wastewaters

Fungal Remediation and Subsequent Methanogenic Digestion of Sixteen Winery Wastewaters

Fungal remediation of Amarula distillery wastewater

Growth of Trametes versicolor on phenol

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