Bioremediation: environmental clean-up through pathway engineering

Singh, Samunder; Kang, Seung-Gul; Mulchandani, Ashok; Chen, Wilfred

doi:10.1016/j.copbio.2008.07.012

Cited by 177 publications

(68 citation statements)

References 58 publications

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“…Mercury-resistant bacteria are an important alternative tool for bioremediation because of their simplicity, lack of secondary contamination, and low cost compared with other treatment technologies (Singh et al 2008). Although P. putida SP1 was present at barely detectable level in oligotrophic marine niche, it still volatilized 89% of total mercury.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang

Chen

Liu

2011

Appl Microbiol Biotechnol

127

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The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 μM HgCl 2 . SP1 was also highly resistant to other metals, including CdCl 2 , CoCl 2 , CrCl 3 , CuCl 2 , PbCl 2 , and ZnSO 4 , and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl 2 and the removal of HgCl 2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg 2+ to volatile and relatively inert monoatomic Hg 0 vapor, was around 5.0. LD 50 of P. putida SP1 to flounder and turbot was 1.5× 10 9 CFU. Biofilm developed by P. putida SP1 was 1-to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl 2 contamination over a broad range of pH.

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Section: Discussionmentioning

confidence: 99%

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang

Chen

Liu

2011

Appl Microbiol Biotechnol

127

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“…Co-expression of precursor glutathione (GSH) along with phytochelatins (PC) resulted in the 10 fold increase in PC that finally increased cadmium accumulation twofold [84]. Natural resistant pathways for heavy metals (Hg and Ar) in microorganisms have been regulated by metalloregulatory protein [85].…”

Section: Bioremediation By Physio-bio-chemical Mechanismmentioning

confidence: 99%

Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes

et al. 2015

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Heavy metals are natural constituents of the environment, but indiscriminate use for human purposes has altered their geochemical cycles and biochemical balance. This results in excess release of heavy metals such as cadmium, copper, lead, nickel, zinc etc. into natural resources like the soil and aquatic environments. Prolonged exposure and higher accumulation of such heavy metals can have deleterious health effects on human life and aquatic biota. The role of microorganisms and plants in biotransformation of heavy metals into nontoxic forms is well-documented, and understanding the molecular mechanism of metal accumulation has numerous biotechnological implications for bioremediation of OPEN ACCESSSustainability 2015, 7 2190 metal-contaminated sites. In view of this, the present review investigates the abilities of microorganisms and plants in terms of tolerance and degradation of heavy metals. Also, advances in bioremediation technologies and strategies to explore these immense and valuable biological resources for bioremediation are discussed. An assessment of the current status of technology deployment and suggestions for future bioremediation research has also been included. Finally, there is a discussion of the genetic and molecular basis of metal tolerance in microbes, with special reference to the genomics of heavy metal accumulator plants and the identification of functional genes involved in tolerance and detoxification.

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“…Although these methods could effectively remove DES from the environments, the whole processes needed specific cells in site, such as cells with ultrasound sample, PbO 2 anode, and stainless steel cathode, or certain equipment including highpressure mercury lamp and ozone generator. Nowadays, transformation of various organic pollutants by microorganisms has been demonstrated to be a more effective and facile, and less expensive way to eliminate pollution in the environments compared to the physicochemical methods (Chang 2008;Singh et al 2008). Lee and Liu (2002) had reported the degradation of other EDCs, 17β-estradiol and its metabolites, by sewage bacteria.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Zhang

Niu

Liao

et al. 2013

Appl Microbiol Biotechnol

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Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/ MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.

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Bioremediation: environmental clean-up through pathway engineering

Cited by 177 publications

References 58 publications

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes

Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

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