Bioreactors for plant cells: hardware configuration and internal environment optimization as tools for wider commercialization

Georgiev, Milen I.; Weber, Jost

doi:10.1007/s10529-014-1498-1

Cited by 53 publications

(36 citation statements)

References 39 publications

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“…The growth of plant tissues or organs under controlled submerged conditions brings other engineering problems related to construction of vessels, realization of mass transfer, mixing and etc. Some of the processes have been implemented in commercial scale, as well [3,52]. With the advance of bioprocess engineering, most of these technological issues have been solved in acceptable level and nowadays many bioreactor systems, as well as cultivation strategies have been developed and successfully applied for cultivation of various differentiated plant in vitro cultures including adventitious roots, transformed hairy roots, embryos, shoots and seedlings.…”

Section: Differentiated In Vitro Systemsmentioning

confidence: 99%

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Vrancheva

Ivanov

Aneva

et al. 2018

Engineering in Life Sciences

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During the last few years there is an increasing demand to the natural biologically active compounds. According to the World Health Organization (WHO) about 11% of the conventional medicines are of plant origin. Nowadays, plant biotechnologies are modern and reliable tool for producing valuable bioactive compounds. Recently, the potential of plant cells as foods also was confirmed. The advantages of plant in vitro systems over the intact plants are well known: growing under controlled and optimized laboratory conditions; independence of climatic and soil differences; preservation of rare and endangered plant species; cultivation in diverse bioreactor systems for increasing production yields of target metabolites. There have been developed many in vitro systems for production of various plant bioactive compounds with potential application in food industries. But potential for industrial implementation of this technology depends on solving problems with the scale‐up of bioreactor cultivation, development of additional approaches for improving/modification of bioactivities of the target plant secondary metabolites, and to find way to exclude or replace in the culture media the carcinogenic plant growth regulator 2,4‐dichlorophenoxyacetic acid (2,4‐D) with its safety analogs, such as α‐naphtaleneacetic acid (NAA) and/or indole‐3‐butyric acid (IBA). The aim of the current mini review is to summarize information about different in vitro systems of edible plants from the Balkan Peninsula with potential for producing food additives and biologically active substances and to describe prospects for successful industrial implementation of this technology.

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Section: Differentiated In Vitro Systemsmentioning

confidence: 99%

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Vrancheva

Ivanov

Aneva

et al. 2018

Engineering in Life Sciences

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“…Although considerable progress in biotechnology of cultured plant cells producing secondary metabolites has been achieved (Khojasteh et al 2014;Georgiev and Weber 2014), the development of new bioengineering methods is a highly important issue for this area of research. miRNAs may be ideally suited for regulating secondary metabolism in cultured plant cells due to their ability to regulate entire gene families.…”

Section: Introductionmentioning

confidence: 99%

New opportunities for the regulation of secondary metabolism in plants: focus on microRNAs

Bulgakov

Авраменко

2015

Biotechnol Lett

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Plant cell cultures are of particular interest in industrial applications as a source of biologically active substances. It is difficult, however, to achieve stable production of secondary metabolites for many plant cell cultures using classical techniques. Novel approaches should be developed for removal of the inhibitor blocks that prevent pathway activation and shift the regulatory balance to the activation of entire biosynthetic pathways. MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in various biological processes. Only recently miRNAs have been demonstrated as active in secondary metabolism regulation. In this work, we summarize recent data on the emerging approaches based on regulation of secondary metabolism by miRNAs.

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“…Phenolic compounds, an important group of secondary metabolites, are reported to play several roles as medicinal agents, showing antioxidant and anti-inflammatory effects (Shetty 2001). Rosmarinic acid (RA) is a common water-soluble phenolic compound found mainly in Lamiaceae and Boraginaceae plant species (Khojasteh et al 2014;Georgiev and Weber 2014). RA, derived from caffeic acid and (R)-(?…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

et al. 2015

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An in vitro approach to the production of rosmarinic acid (RA), a medicinally important caffeic acid ester, in a cell suspension culture (CSC) of Satureja khuzistanica Jamzad (Lamiaceae) has been investigated for the first time. The CSC was established from friable calli derived from shoot tip explants in Gamborg's B5 liquid medium supplemented with 30 g/L sucrose, 20 mg/L L-glutamine, 200 mg/L casein hydrolysate, 5 mg/L benzyladenine (BA) and 1 mg/L indole-3-butyric acid (IBA). The effect of nitrogen source (KNO 3 and (NH 4 ) 2 SO 4 ) and their different concentrations on the fresh and dry weight (g/L), as well as RA content (mg/g dry weight)were measured. CSC growth measurements indicated a maximum specific cell growth rate of 1.5/day, a doubling time of 7.6 days and a high percentage of cell viability (96.4 %) throughout the growth cycle. Maximum cell fresh weight (353.5 g/L), dry weight (19.7 g/L) and RA production (180.0 mg/g) were attained at day 21 of culture. Cell growth and RA content were affected by nitrogen deficiency. Media containing 8.3 mM of total nitrogen ( of B5 standard medium) led to a minimum cell fresh weight (243.0 g/ L), dry weight (17.4 g/L) and RA content (38.0 mg/g) after 21 days. The established CSC provided useful material for further optimization experiments aimed at a large-scale production of RA.

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Bioreactors for plant cells: hardware configuration and internal environment optimization as tools for wider commercialization

Cited by 53 publications

References 39 publications

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

New opportunities for the regulation of secondary metabolism in plants: focus on microRNAs

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

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