Bioprinting transgenic plant cells for production of a recombinant biodefense agent

Varma, Anika; Gemeda, Hawi B.; Mj, McNulty; McDonald, Karen A.; Nandi, Somen; Jm, Knipe

doi:10.1101/2021.02.01.429263

Cited by 2 publications

(2 citation statements)

References 51 publications

(78 reference statements)

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“…Varma et al reported 3D bioprinting of transgenic Oryza sativa (rice), which produces recombinant butyrylcholinesterase (BChE) 140 as a prophylactic/therapeutic against organophosphate nerve agent poisoning, cocaine toxicity, and neurodegenerative diseases like Alzheimer’s, and cells were immobilized in a polyethylene glycol-based hydrogel. 141 The cells maintained viability similar to those in suspension cultures. They displayed similar sugar consumption trends for 14 days in the semisolid matrix, undergoing a growth phase from days 0–6, a BChE production phase in a sugar-free medium from days 6–12, and a growth/recovery phase from days 12–14.…”

Section: Innovations In 3d Printing Driving the Future Of Bioengineer...mentioning

confidence: 66%

“…Varma et al reported 3D bioprinting of transgenic Oryza sativa (rice), which produces recombinant butyrylcholinesterase (BChE) as a prophylactic/therapeutic against organophosphate nerve agent poisoning, cocaine toxicity, and neurodegenerative diseases like Alzheimer’s, and cells were immobilized in a polyethylene glycol-based hydrogel . The cells maintained viability similar to those in suspension cultures.…”

Section: Innovations In 3d Printing Driving the Future Of Bioengineer...mentioning

confidence: 99%

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The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

Verma,

Khanna,

Kumar

et al. 2023

ACS Omega

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The scope of three-dimensional printing is expanding rapidly, with innovative approaches resulting in the evolution of state-of-the-art 3D bioprinting (3DbioP) techniques for solving issues in bioengineering and biopharmaceutical research. The methods and tools in 3DbioP emphasize the extrusion process, bioink formulation, and stability of the bioprinted scaffold. Thus, 3DbioP technology augments 3DP in the biological world by providing technical support to regenerative therapy, drug delivery, bioengineering of prosthetics, and drug kinetics research. Besides the above, drug delivery and dosage control have been achieved using 3D bioprinted microcarriers and capsules. Developing a stable, biocompatible, and versatile bioink is a primary requisite in biofabrication. The 3DbioP research is breaking the technical barriers at a breakneck speed. Numerous techniques and biomaterial advancements have helped to overcome current 3DbioP issues related to printability, stability, and bioink formulation. Therefore, this Review aims to provide an insight into the technical challenges of bioprinting, novel biomaterials for bioink formulation, and recently developed 3D bioprinting methods driving future applications in biofabrication research.

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Section: Innovations In 3d Printing Driving the Future Of Bioengineer...mentioning

confidence: 66%

Section: Innovations In 3d Printing Driving the Future Of Bioengineer...mentioning

confidence: 99%

The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

Verma,

Khanna,

Kumar

et al. 2023

ACS Omega

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Green Bioprinting with Layer-by-Layer Photo-Crosslinking: A Designed Experimental Investigation on Shape Fidelity and Cell Viability of Printed Constructs

Thakare

Jerpseth

Pei

et al. 2022

JMMP

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Process variables of bioprinting (including extrusion pressure, nozzle size, and bioink composition) can affect the shape fidelity and cell viability of printed constructs. Reported studies show that increasing extrusion pressure or decreasing nozzle size would decrease cell viability in printed constructs. However, a smaller nozzle size is often necessary for printing constructs of higher shape fidelity, and a higher extrusion pressure is usually needed to extrude bioink through nozzles with a smaller diameter. Because values of printing process variables that increase shape fidelity can be detrimental to cell viability, the optimum combination of variables regarding both shape fidelity and cell viability must be determined for specific bioink compositions. This paper reports a designed experimental investigation (full factorial design with three variables and two levels) on bioprinting by applying layer-by-layer photo-crosslinking and using the alginate-methylcellulose-GelMA bioink containing algae cells. The study investigates both the main effects and interaction effects of extrusion pressure, nozzle size, and bioink composition on the shape fidelity and cell viability of printed constructs. Results show that, as extrusion pressure changed from its low level to its high level, shape fidelity and cell viability decreased. As nozzle size changed from its low level to its high level, shape fidelity decreased while cell viability increased. As bioink composition changed from its low level (with more methylcellulose content in the bioink) to its high level (with less methylcellulose content in the bioink), shape fidelity and cell viability increased.

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Bioprinting transgenic plant cells for production of a recombinant biodefense agent

Cited by 2 publications

References 51 publications

The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

The Art of Building Living Tissues: Exploring the Frontiers of Biofabrication with 3D Bioprinting

Green Bioprinting with Layer-by-Layer Photo-Crosslinking: A Designed Experimental Investigation on Shape Fidelity and Cell Viability of Printed Constructs

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