Biophysical properties of a chloride channel in the apical membrane of a secretory epithelial cell

A Cl- channel activated by parathyroid hormone in rabbit renal proximal tubule cells.

“…The selectivity of Cl-channel to these ions is similar to the previous report (6). Addition ofDIDS (l0-4 M) from inside the membrane completely blocked the currents between the potential of -60 to +60 mV (Fig.…”

A Cl- channel activated by parathyroid hormone in rabbit renal proximal tubule cells.

“…The selectivity of Cl-channel to these ions is similar to the previous report (6). Addition ofDIDS (l0-4 M) from inside the membrane completely blocked the currents between the potential of -60 to +60 mV (Fig.…”

Bicarbonate permeability of the outwardly rectifying anion channel

“…The slope conductances estimated at -30, 0 and +70 mV (23, 30 and 73 pS, respectively) are similar to those reported for T84 cells (25,41 and 71 pS, respectively; [31]), cultured airway cells (29,30 and 62 pS; [65]: 21, 50 and 71 pS; [22]), and cultured sweat ducts (25, 38 and 80 pS; [5]). The data are also consistent with preliminary reports of outwardly rectifying anion channels in rat epididymal cells [2], Necturus small intestine [24] and placental trophoblast [15].…”

Cytoskeletal Actin Gates a Cl−Channel in Neocortical Astrocytes

“…Both inward and outward currents (Cl Ϫ current from the pipette to the bath) were reduced when the concentration of NaCl in the bath was lower than that in the pipette, as illustrated in Figure 3, A and B. Interestingly, reduction in inward current amplitude after an increase in bath NaCl with respect to pipette NaCl was not observed, as demonstrated in Figure 3. This paradoxical reduction in both inward and outward current in asymmetrical solutions has been reported in selectivity experiments for outwardly rectifying Cl Ϫ channels in epithelial cells (Halm et al, 1988;Tabcharani et al, 1989). Figure 4 demonstrates block of the outward rectifier by three agents known to inhibit single-channel and whole-cell anion currents in other preparations, including morphology-dependent whole-cell Cl Ϫ currents (Lascola and Kraig, 1996).…”

A Cl- channel activated by parathyroid hormone in rabbit renal proximal tubule cells.

“…The selectivity of Cl-channel to these ions is similar to the previous report (6). Addition ofDIDS (l0-4 M) from inside the membrane completely blocked the currents between the potential of -60 to +60 mV (Fig.…”

Bicarbonate permeability of the outwardly rectifying anion channel

“…The slope conductances estimated at -30, 0 and +70 mV (23, 30 and 73 pS, respectively) are similar to those reported for T84 cells (25,41 and 71 pS, respectively; [31]), cultured airway cells (29,30 and 62 pS; [65]: 21, 50 and 71 pS; [22]), and cultured sweat ducts (25, 38 and 80 pS; [5]). The data are also consistent with preliminary reports of outwardly rectifying anion channels in rat epididymal cells [2], Necturus small intestine [24] and placental trophoblast [15].…”

Cytoskeletal Actin Gates a Cl−Channel in Neocortical Astrocytes

“…Both inward and outward currents (Cl Ϫ current from the pipette to the bath) were reduced when the concentration of NaCl in the bath was lower than that in the pipette, as illustrated in Figure 3, A and B. Interestingly, reduction in inward current amplitude after an increase in bath NaCl with respect to pipette NaCl was not observed, as demonstrated in Figure 3. This paradoxical reduction in both inward and outward current in asymmetrical solutions has been reported in selectivity experiments for outwardly rectifying Cl Ϫ channels in epithelial cells (Halm et al, 1988;Tabcharani et al, 1989). Figure 4 demonstrates block of the outward rectifier by three agents known to inhibit single-channel and whole-cell anion currents in other preparations, including morphology-dependent whole-cell Cl Ϫ currents (Lascola and Kraig, 1996).…”

A Cl- channel activated by parathyroid hormone in rabbit renal proximal tubule cells.

“…The selectivity of Cl-channel to these ions is similar to the previous report (6). Addition ofDIDS (l0-4 M) from inside the membrane completely blocked the currents between the potential of -60 to +60 mV (Fig.…”

Bicarbonate permeability of the outwardly rectifying anion channel

“…The slope conductances estimated at -30, 0 and +70 mV (23, 30 and 73 pS, respectively) are similar to those reported for T84 cells (25,41 and 71 pS, respectively; [31]), cultured airway cells (29,30 and 62 pS; [65]: 21, 50 and 71 pS; [22]), and cultured sweat ducts (25, 38 and 80 pS; [5]). The data are also consistent with preliminary reports of outwardly rectifying anion channels in rat epididymal cells [2], Necturus small intestine [24] and placental trophoblast [15].…”

Cytoskeletal Actin Gates a Cl−Channel in Neocortical Astrocytes

“…Both inward and outward currents (Cl Ϫ current from the pipette to the bath) were reduced when the concentration of NaCl in the bath was lower than that in the pipette, as illustrated in Figure 3, A and B. Interestingly, reduction in inward current amplitude after an increase in bath NaCl with respect to pipette NaCl was not observed, as demonstrated in Figure 3. This paradoxical reduction in both inward and outward current in asymmetrical solutions has been reported in selectivity experiments for outwardly rectifying Cl Ϫ channels in epithelial cells (Halm et al, 1988;Tabcharani et al, 1989). Figure 4 demonstrates block of the outward rectifier by three agents known to inhibit single-channel and whole-cell anion currents in other preparations, including morphology-dependent whole-cell Cl Ϫ currents (Lascola and Kraig, 1996).…”