Biophysical Aspects of Liver Aeration by Vascular Persufflation With Gaseous Oxygen

Minor, Th.; Klauke, H.; Vollmar, Brigitte; Isselhard, W.; Menger,

doi:10.1097/00007890-199706270-00025

Cited by 39 publications

(29 citation statements)

References 10 publications

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“…Minor et al [14,42] showed in their studies involving liver preservation that VSOP can provide energetic homeostasis by elevated ATP tissue concentrations and a normalized redox status leading to reduced lipid peroxidation. Here, in our VSOP group, a higher GSH/GSSG ratio and reduced lipid peroxidation as reflected in lower tissue TBARS concentrations were observed compared to the OMP group.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Kalenski

Mancina²,

Paschenda³

et al. 2016

Eur Surg Res

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Background/Aim: The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK). Materials and Methods: Kidneys from German Landrace pigs (n = 5/group) were retrieved and washed out with either Ecosol or HTK after 45 min of clamping of the renal pedicle. As controls, kidneys without warm ischemia, cold stored for 24 h in HTK, were employed. Following 24 h of preservation by VSOP, OMP, CS-Ecosol, or CS-HTK, renal function and damage were assessed during 1 h using the isolated perfused porcine kidney model. Results: During reperfusion, urine production was significantly higher in the VSOP and OMP groups than in the CS-HTK group; however, only VSOP could demonstrate lower urine protein concentrations and fractional excretion of sodium, which did not differ from the non-warm-ischemia-damaged control group. VSOP, CS-Ecosol, and controls showed better maintenance of the acid-base balance than CS-HTK. Reduced lipid peroxidation, as reflected in postreperfusion tissue thiobarbituric acid-reactive substance levels, was observed in the VSOP group compared to the OMP group, and the VSOP and CS-Ecosol groups had concentrations similar to the controls. The ratio of reduced to oxidized glutathione was higher in the VSOP, OMP, and CS-Ecosol groups than in the CS-HTK group and controls, with a higher ratio in the VSOP than in the OMP group. Conclusion: VSOP was associated with mitigation of oxidative stress in comparison to OMP and CS. Preservation of warm-ischemia-damaged porcine kidneys by VSOP was improved compared to OMP and CS, and was comparable to preservation of non-warm-ischemia-damaged cold-stored kidneys.

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Section: Discussionmentioning

confidence: 99%

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Kalenski

Mancina²,

Paschenda³

et al. 2016

Eur Surg Res

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“…Moreover, the influence of continuous oxygenation of the grafts should be isolated by nonperfusion related aeration of the liver by mere gaseous oxygen insufflation during hypothermic storage, as was proposed earlier [16,17].…”

Section: Discussionmentioning

confidence: 99%

Role of perfusion medium, oxygen and rheology for endoplasmic reticulum stress-induced cell death after hypothermic machine preservation of the liver

Manekeller¹,

Schuppius²,

Stegemann³

et al. 2007

Transplant Int

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“…These reports and the present study show that the granulosa cells and oocytes are exposed to suboptimal conditions and are injured by ischemic conditions. Generally, one of the key factors in organ preservation is restoration of the ATP concentration in the tissues during preservation [9,21]. It has been shown that maintaining the preservation solution at a low temperature is beneficial for restoring the developmental competence of oocytes obtained from ovaries preserved for a long period of time [2][3][4][5][6][7][8].…”

Section: Discussionmentioning

confidence: 99%

Effect of Modification of Ovary Preservation Solution by Adding Glucose on the Maturation and Development of Pig Oocytes after Prolonged Storage

Sakamoto

Iwata

Sato

et al. 2006

Journal of Reproduction and Development

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Abstract. Oocytes lose their developmental competence during prolonged storage of the ovary. In the present study, we supplemented the preservation solution for pig ovaries (phosphate buffered saline, PBS) with glucose and preserved the ovaries for 6 h at 25 C. Subsequently, we examined the glucose concentration of the follicular fluid (FF), pH of the FF, survival rate of the granulosa cells, and maturation and developmental competence of oocytes after storage. During storage, the glucose concentration of the FF (2.1 mM), pH of the FF (7.4), and survival rate of the granulosa cells (69.5%) rapidly decreased (glucose concentration: under 1.1 mM; pH: 6.8; and survival rate: 43%). On the other hand, when the preservation solution was supplemented with glucose (15 mM), the glucose concentration of the FF increased and the survival rate of the granulosa cells improved, although the pH of the FF decreased further (from 6.8 to 6.6). In addition, supplementation with glucose significantly improved the rates of oocytes at metaphase II (0 h: 65.0%; 6 h without glucose: 23.8%; and 6 h with glucose: 43.8%) and attenuated the decline in the rates of fertilization and development that resulted from prolonged storage, although there were no significant differences. In conclusion, modification of the preservation solution by the addition of glucose increased the glucose concentration of the FF and improved the rate of maturation of pig oocytes. Key words: Ovary preservation, Glucose, Pig oocyte (J. Reprod. Dev. 52: [669][670][671][672][673][674] 2006) ocyte quality is a major factor that affects the developmental rate of in vitro matured/in vitro fertilized oocytes to the blastocyst stage. For in vitro embryo production in domestic animals, oocytes are generally aspirated from ovaries collected at a slaughterhouse. These ovaries are preserved in physiological saline or phosphate buffered saline (PBS) at 30-35 C for a predetermined time period b e f o r e o o c y t e c o l l e c t i o n . D u r i n g o v a r y preservation, the supply of oxygen and blood flow to the ovaries is halted; thus, the oocytes are maintained under ischemic conditions. During bovine ovary preservation, a decrease in both the glucose concentration of the folliclular fluid (FF) and the survival rate of the granulosa cells in the follicle and an increase in the potassium ion (K + ) concentration of the FF indicate that the oocyte and surrounding cells are exposed to suboptimal conditions compared with the in vivo conditions [1,2]. In general, to prevent ischemic injuries, a delicate balance of the energy levels within the ovary during storage is thought to be achieved by two strategies, (1) reducing the activity of energyconsuming processes, and (2) increasing the rate of energy production (via glycolysis). Lowering the

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Biophysical Aspects of Liver Aeration by Vascular Persufflation With Gaseous Oxygen

Abstract: Our results indicate that the oxygen concentration of the persufflation gas rather than the persufflation pressure is a determinant of successful tissue oxygenation during cold storage.

Cited by 39 publications

References 10 publications

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Role of perfusion medium, oxygen and rheology for endoplasmic reticulum stress-induced cell death after hypothermic machine preservation of the liver

Effect of Modification of Ovary Preservation Solution by Adding Glucose on the Maturation and Development of Pig Oocytes after Prolonged Storage

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