Biomimetic layer-by-layer templates for calcium phosphate biomineralization

Abdelkebir, Khalil; Morin-Grognet, Sandrine; Gaudière, Fabien; Coquerel, Gérard; Labat, Béatrice; Atmani, H.; Ladam, Guy

doi:10.1016/j.actbio.2012.05.035

Cited by 30 publications

(19 citation statements)

References 57 publications

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“…Despite the advantages offered, the number of image mapping based Raman studies in the field is limited with only a small number of studies looking at or relating to the ageing of calcium phosphate cement implants known to the authors21222324. In part, the low level of use in the literature has been due to technological limitations with long spectral acquisition times, giving poor data quality.…”

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confidence: 99%

Visualising phase change in a brushite-based calcium phosphate ceramic

Bannerman

Williams

Cox

et al. 2016

Sci Rep

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The resorption of brushite-based bone cements has been shown to be highly unpredictable, with strong dependence on a number of conditions. One of the major factors is phase transformation, with change to more stable phases such as hydroxyapatite affecting the rate of resorption. Despite its importance, the analysis of phase transformation has been largely undertaken using methods that only detect crystalline composition and give no information on the spatial distribution of the phases. In this study confocal Raman microscopy was used to map cross-sections of brushite cylinders aged in Phosphate Buffered Saline, Foetal Bovine Serum, Dulbecco’s – Minimum Essential Medium (with and without serum). Image maps showed the importance of ageing medium on the phase composition throughout the ceramic structure. When aged without serum, there was dissolution of the brushite phase concomitant to the deposition of octacalcium phosphate (OCP) around the periphery of the sample. The deposition of OCP was detectable within five days and reduced the rate of brushite dissolution from the material. The use of serum, even at a concentration of 10vol% prevented phase transformation. This paper demonstrates the value of confocal Raman microscopy in monitoring phase change in biocements; it also demonstrates the problems with assessing material degradation in non-serum containing media.

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confidence: 99%

Visualising phase change in a brushite-based calcium phosphate ceramic

Bannerman

Williams

Cox

et al. 2016

Sci Rep

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“…We assumed, the oCS‐Col I multilayers coated on the PLGA scaffold could attract and enrich calcium ions and provide nucleation sites, and thus accelerating and improving the deposition and homogeneous distribution of apatite crystals as compared to the pure PLGA scaffold, which was confirmed in this study. Similar findings were made by Abdelkebir et al, who explored the potency of other multilayer films as templates for controlling biomineralization. Another important finding was the greatly improved mechanical strength of PLGA scaffolds through the deposition of HAP fostered by the oCS‐Col I multilayer coating.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, layer by layer technique (LbL) has become of great interest for the design of biomimetic coatings, owing to the possibility of incorporating a variety of bioactive species therein . For example, Abdelkebir et al reported that LbL films made of anionic CS and cationic poly(L‐lysine) (PLL) with different terminal layers are effective templates for controlling biomineralization including the deposition kinetics and structure of calcium phosphate coatings . In our previous studies, we developed a unique multilayer system made by LbL based on Col I with intrinsic cross‐linking capacity by the use of oxidized CS (oCS) as polyanion .…”

Section: Introductionmentioning

confidence: 99%

“…33 For example, Abdelkebir et al reported that LbL films made of anionic CS and cationic poly(L-lysine) (PLL) with different terminal layers are effective templates for controlling biomineralization including the deposition kinetics and structure of calcium phosphate coatings. 34 In our previous studies, we developed a unique multilayer system made by LbL based on Col I with intrinsic cross-linking capacity by the use of oxidized CS (oCS) as polyanion. 35,36 These novel kinds of multilayer films expressed an excellent Col I fibril formation because of the presence of CS and had an improved stability.…”

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Biomineralization improves mechanical and osteogenic properties of multilayer‐modified PLGA porous scaffolds

Kong

Wei

Groth

et al. 2018

J Biomedical Materials Res

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Poly-(lactide-co-glycolide acid) (PLGA) has been widely investigated as scaffold material for bone tissue engineering owing to its biosafety, biodegradability, and biocompatibility. However, the bioinert surface of PLGA may fail in regulating cellular behavior and directing osteointegration between the scaffold and the host tissue. In this article, oxidized chondroitin sulfate (oCS) and type I collagen (Col I) were assembled onto PLGA surface via layer by layer technique (LbL) as an adhesive coating for the attachment of inorganic minerals. The multilayer-modified PLGA scaffold was mineralized in vitro to ensure the deposition of nanohydroxyapatite (nHAP). It was found that nHAP crystals were more uniformly and firmly attached on the multilayer-modified PLGA as compared with the pure PLGA scaffold, which remarkably improved PLGA surface and mechanical properties. Additionally, in vitro biocompatibility of PLGA scaffold, in terms of bone mesenchymal stem cells (BMSCs) attachment, spreading and proliferation was greatly enhanced by nHAP coating and multilayer deposition. Furthermore, the fabricated composite scaffold also shows the ability to promote the osteogenic differentiation of BMSCs through the up-regulation of osteogenic marker genes. Thus, this novel biomimetic composite scaffold might achieve a desirable therapeutic result for bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2714-2725, 2018.

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“…Mineralized polymeric matrices have emerged as promising tools to uncover the complex roles of the cellular microenvironment in regulating the diverse phenotypic activities of stem and progenitor cells related to the development, repair, regeneration, and remodeling of bone tissue . Most of the studies focused on the apatite formation on gels, scaffolds, fibers, or grafted films . Several pioneering works have tried to understand and shown that the role of chemical motifs should be mediated by other matrix variables, such as pore size and the density of hydrophobic motifs, specifically for the mineralization of a three dimensional matrix .…”

Section: Introductionmentioning

confidence: 99%

Biomineralized hyaluronic acid/poly(vinylphosphonic acid) hydrogel for bone tissue regeneration

Kim

Park

2014

J of Applied Polymer Sci

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Novel biomineralized hydrogels composed of hyaluronic acid (HA) and vinyl phosphonic acid (VPAc) were designed with the aim of developing a biomimetic hydrogel system to improve bone regeneration by local delivery of a protein drug including bone morphogenetic proteins. We synthesized crosslinked hydrogels composed of methacrylated HA and poly(VPAc) [P(VPAc)], which serves as a binding site for calcium ions during the mineralization process. The HA/P(VPAc) hydrogels were biomineralized by a urea‐mediation method to create functional polymer hydrogels that can deliver the protein drug and mimic the bone extracellular matrix. The water content of the hydrogels was influenced by the HA/P(VPAc) composition, crosslinking density, biomineralization, and ionic strength of the swelling media. All HA/P(VPAc) hydrogels maintained more than 84% water content. Enzymatic degradation of HA/P(VPAc) hydrogels was dependent on the concentration of hyaluronidase and the crosslinking density of the polymer network within the hydrogel. In addition, the release behavior of bovine serum albumin from the HA/PVPAc hydrogels was mainly influenced by the drug loading content, water content, and biomineralization of the hydrogels. In a cytotoxicity study, the HA/P(VPAc) and biomineralized HA/P(VPAc) hydrogels did not significantly affect cell viability. These results suggest that biomineralized HA/P(VPAc) hydrogels can be tailored to create a biomimetic hydrogel system that promotes bone tissue repair and regeneration by local delivery of protein drugs. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 41194.

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Biomimetic layer-by-layer templates for calcium phosphate biomineralization

Cited by 30 publications

References 57 publications

Visualising phase change in a brushite-based calcium phosphate ceramic

Visualising phase change in a brushite-based calcium phosphate ceramic

Biomineralization improves mechanical and osteogenic properties of multilayer‐modified PLGA porous scaffolds

Biomineralized hyaluronic acid/poly(vinylphosphonic acid) hydrogel for bone tissue regeneration

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