Biometria corporal e parâmetros hematológicos de Trachemys scripta elegans e Trachemys dorbignyi (Testudines: Emydidae) criadas em cativeiro em Petrolina, Pernambuco

Abstract

ABSTRACT.-Gradela A., Souza V.N., Queiroz M.M., Constantino A.C., Bandeira C.G. This study aimed to evaluate the body biometry and hematological profile of Trachemys scripta elegans (N=28) and Trachemys dorbignyi (N=22) reared in captivity in the Brazilian submedium northeastern semi-arid region in the Valley of the São Francisco river. It aimed to establish basic health blood values and generate useful data on the comparative physiology of Testudines. After 120-day adaptation and 24-hour fasting, 2.5mL of blood were collected from the dorsal occipital sinus and deposited into a tube with sodium heparin for evaluation, following, of hematological levels. The red blood cell count (RBC) and GLC was conducted in a Neubauer chamber, the hemoglobin level (HGB) was supplied by the cyanmethemoglobin method and the hematocrit (HCT) was obtained by the microhematocrit technique. Based on the RBC, the hematimetric were mathematically established. Body biometry were also evaluated: a) body mass (BM, g); b) maximum dimensions of the carapace [length (MLC, cm) and width (MWC, cm)]; c) maximum dimensions of plastron [length (MLP, cm) and width (MWP, cm)]; d) total length of tail (TLT, cm); e) linear length from the base of the tail to the cloacal orifice (LPrC, cm); f) linear length from the cloacal orifice to the extremity of the tail (LPoC, cm). T. scripta elegans showed higher values (P<0.05) for biometrics, while TLT and LPrC were higher (P<0.05) in T. dorbignyi. The hematological values did not differ (P>0.05) among species. The results show that most of the variation found between T. scripta elegans and T. dorbignyi is explained by the biometric variables and that some hematologic correlations characterize interspecies differences. It was conclude that the results shed light on benchmarks for these species kept in captivity in the northeastern semi-arid region and serve as a model for intra and interspecies comparative physiology.

Reproductive biology, morphology, and morphometry of ovaries and oviducts of Trachemys scripta elegans in Brazilian Cerrado

“…In all groups, there was a positive and harmonic development among mass, carapace, and plastron, which were positively correlated. These data corroborate with the results of Gradela et al (2017b) and diverge from Gradela et al (2017a) who observed development uniformity only between body mass and carapace length and width of the plastron, and between the carapace and plastron lengths.…”

“…Average body mass values did not differ from Perez-Santigosa et al (2008) and Gradela et al (2017a), but were slightly higher than Vieira & Costa (2006); lower than those of Gradela et al (2017b) and well above those of Taniguchi et al (2017). The maximum carapace length did not differ from Perez-Santigosa et al (2008) and Gradela et al (2017aGradela et al ( , 2017b, but was slightly higher than described by Vieira & Costa (2006) and lower than Taniguchi et al (2017). In all groups, there was a positive and harmonic development among mass, carapace, and plastron, which were positively correlated.…”

PROFILE OF CONSTITUENTS ENZYMATIC SERUM IN TORTOISES Chelonoidis carbonaria (REPTILIA, TESTUDINIDAE) HELD IN CAPTIVITY

“…7,0 (±9,0) U/L (SAMOUR, 1986;CARPENTER, 2005), C. elegans: (8) (±10) U/L, C. radiata: 9,0 (±11) U/L. and C. carbonaria 9,0 (±5) U/L and in Phrynops geoffroanus (11 to 39) U/L (GRADELA, et al, 2017), but they're found within the values established for reptiles, which is inferior to 20 U/L (MARTÍNEZ-SILVESTRE et al, 2013).…”

“…In C. carbonaria, the average of amylase found in the sanguine serum was of 3,50 (±2,10) U/L. Discrepant results, with markedly superior averages for Phrynops geoffroanus (193 a 794) U/L (GRADELA, et al, 2017). In reptiles, the amylase is used on the diagnoses of pancreatic lesions ( 25).…”

Consideraciones para la obtención de sangre en tortugas: sitios de venopunción y anticoagulantes

“…A pesar de esto, la heparina de sodio utilizada en este estudio mostró resultados favorables al mantener las muestras sanguíneas de todas las especies analizadas en óptimas condiciones para análisis hematológicos. Estos resultados apoyan diferentes reportes donde también se ha usado a la heparina de sodio para la medición de parámetros hematológicos de tortugas de las especies T. scripta elegans, T. dorbingyi, Podocnemis expansa, Chelonoidis chathamensis, Caretta caretta y Geochelone radiata (11,29,30,31,32,33) semiárido nordestino brasileiro, visando estabelecer valores sanguíneos básicos de saúde e gerar dados úteis na fisiologia comparativa de Testudines. Após 120 dias de adaptação e jejum de 24 horas, 2,5 mL de sangue foram coletados do seio occipital dorsal e depositados em tubo com heparina sódica para a avaliação, na sequência, dos níveis hematologicos.…”