Biomedical applications of the ESRF synchrotron-based microspectroscopy platform

Bohic, Sylvain; Cotte, Marine; Salomé, Murielle; Fayard, Barbara; Kuehbacher, Markus; Cloetens, Peter; Martı́nez-Criado, G.; Tucoulou, R.; Susini, J.

doi:10.1016/j.jsb.2011.12.006

Cited by 81 publications

(52 citation statements)

References 79 publications

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“…In the present study, X-ray fluorescence (XRF) signal of radioiodinated siRNAs could be exploited to localize them. Indeed, this approach has several advantages; i) synchrotron nanoprobe allows very low detection limit with the best spatial resolution; therefore it gives a unique probe with the highest sensitivity for intracellular imaging compared to other spatially resolved techniques such as electron or proton microprobe [35]. ii) Iodine is a rare component in the body, principally found within the thyroid gland and any iodine peak detected from control tissues or from the backing polymer thin foil, therefore iodine atom can be used as a tracer for synchrotron X-ray fluorescence and typical cryosection can be analyzed and correlated to adjacent section stained for histological purposes.…”

Section: Discussionmentioning

confidence: 99%

In vivo siRNA distribution and pharmacokinetics assessed by nuclear imaging are modulated according to radiolabelling site

Garin

Ahmadi

Gauchez

et al. 2015

Nuclear Medicine and Biology

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Section: Discussionmentioning

confidence: 99%

In vivo siRNA distribution and pharmacokinetics assessed by nuclear imaging are modulated according to radiolabelling site

Garin

Ahmadi

Gauchez

et al. 2015

Nuclear Medicine and Biology

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“…Regardless, the continuing improvements to XFM technology enables the probing of metal distributions at nanoscale resolution whilst maintaining sensitivity [26], which is paramount for assessing the toxicity of metals that occur at extremely low concentrations.…”

Section: Imaging Biological Exposuresmentioning

confidence: 99%

Multielemental bioimaging of tissues in children's environmental health research

Austin

Niedzwiecki

Arora

2016

Current Opinion in Pediatrics

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Purpose of review Metals play major roles in children's health and are associated with negative health outcomes via deficiency, overload or toxicity. Constantly evolving analytical technology can provide new insight into how metal metabolism and exposure biology are intertwined in a range of biological matrices. Recent findings Exposure can occur prenatally as many metals cross the placental barrier. The placenta is permeable to many metal species, some through tightly regulated transporters, and others because of a limited capacity for detoxification. Post-birth, metal exposure continues to exert long-term health effects, ranging from exposure to exogenous heavy metals, such as lead, to overload of otherwise essential metals, including manganese. Increasing evidence supports the existence of critical developmental windows when susceptibility to toxicants and nutritional deficiencies is highest. Elemental imaging technology provides micro-spatial information on metal uptake and retention across tissue architecture, which provides important insights into exposure and biologic response. Summary Imaging the spatial distribution of elements, both essential and toxic, provides information that bulk measures cannot, including cell-specific distributions and timing of exposure.

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“…By X-ray fluorescence microscopy it was discovered that neural cells redistribute significant pools of copper from their cell bodies to the periphery upon calcium activation [51]. Further state-of-the-art examples of cellular X-ray fluorescence microscopy studies can be found in [52], while [53] gives an example of the use of X-ray fluorescence microscopy on brain tissue slices, and the associated methodologies of sample preparation. Finally, it should 3.2 X-Ray Fluorescence Microscopy and X-Ray Spectro-Microscopy be mentioned that if augmented by X-ray spectro-microscopy, the energy-dependent fluorescence yield (near edge X-ray absorption spectrum) can be analyzed to retrieve the oxidation state of the element [54].…”

Section: Fzp-based X-ray Microscopymentioning

confidence: 99%

X-Ray Microscopy for Neuroscience: Novel Opportunities by Coherent Optics

Salditt¹,

Dučić²

2014

Neuromethods

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X-ray microscopy and tomography can provide the three-dimensional density distribution within cells and tissues without staining and slicing. In addition, chemical information-i.e. the elemental distributioncan be retrieved by X-ray spectro-microscopy based on contrast variation around photon absorption edges and X-ray fluorescence. For a long time, X-ray microscopy has been limited in resolution by the fabrication of zone plate lenses, in particular for the hard X-ray range, which is needed to penetrate multicellular samples. Recent progress in X-ray optics and lensless coherent imaging now pave the way for enhanced imaging tools in neuroscience.

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Biomedical applications of the ESRF synchrotron-based microspectroscopy platform

Cited by 81 publications

References 79 publications

In vivo siRNA distribution and pharmacokinetics assessed by nuclear imaging are modulated according to radiolabelling site

In vivo siRNA distribution and pharmacokinetics assessed by nuclear imaging are modulated according to radiolabelling site

Multielemental bioimaging of tissues in children's environmental health research

X-Ray Microscopy for Neuroscience: Novel Opportunities by Coherent Optics

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