Biomechanical regulation of planar cell polarity in endothelial cells

Wang, Caihong; Qu, Kai; Wang, Jing; Qin, Rui; Li, Bingyi; Qiu, Juhui; Wang, Guixue

doi:10.1016/j.bbadis.2022.166495

Cited by 5 publications

(2 citation statements)

References 296 publications

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“…Only once the entire edge was covered did the proliferation direction change in some locations where cells started to divide perpendicularly and climb up the edge, to form a multicellular cord that would then bend and fold the gel. The change in direction observed could be mediated by Planar Cell Polarization (PCP), a polarity axis and signaling pathway that is critical in vascular morphogenesis 58 . Studies have indicated that PAR-3 regulates PCP and can be modulated by fluid flow 59 .…”

Section: Discussionmentioning

confidence: 99%

Microvascular engineering for the development of a non-embedded liver sinusoid with a lumen: when endothelial cells do not lose their edge

Monroy-Romero,

Nieto-Rivera,

Xiao

et al. 2023

Preprint

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Vascular engineering seeks to generate reliable models for studying cellular interactions in the context of preserving homeostasis and disease development. However, current systems mainly focus on recapitulating large blood vessels embedded in thick extracellular matrix gels or do not address discontinuous capillaries, crucial microvascular structures. By adapting a Marangoni flow-driven protein densification process on GelTrex domes, we promoted the self-organization of endothelial cells into mature lumenized vessels by anisotropic directional organization following an edge micropattern. Our findings revealed a sequential morphogenetic process leading to microvessels with both murine and human immortalized liver sinusoidal endothelial cells (LSECs). Differential behaviors were found on the whole sample and cell migration, proliferation and polarization were guided by this pattern to form a long multicellular cord. Cells forming this structure deformed large regions of the dome, generating a wave-like fold all around it, hinged in a laminin depletion zone. This wrapped the cell cords with proteins from the gel which marked the beginning of a lumenogenesis process, regulated by a change in the apico-basal polarization of the cord cells, followed by maturation of tight junctions, remodeling of the extracellular matrix, and formation of a lumen; all steps reported inin vivovessel development. Furthermore, we demonstrate that the geometry of the vessels can be controlled by initial topography of the gel. We believe our simple fabrication method, guiding an autonomous self-organization of vessels without the need for supporting cells or complex prefabricated scaffolds, is suitable for future integration into microphysiological systems of discontinuous, fenestrated capillaries.

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Section: Discussionmentioning

confidence: 99%

Microvascular engineering for the development of a non-embedded liver sinusoid with a lumen: when endothelial cells do not lose their edge

Monroy-Romero,

Nieto-Rivera,

Xiao

et al. 2023

Preprint

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“…The human cardiac is a marvelous fluidic system, which is very sensitive to biomechanical and biochemical. As a dynamic biomechanical trait, fluid shear stress is mainly derived from blood flow in the heart ( Wang et al, 2022 ). Multiphasic fluid is contained about many parts.…”

Section: Dynamic Biomechanical Traitsmentioning

confidence: 99%

Dynamic and static biomechanical traits of cardiac fibrosis

Liu

Fan²,

Jin³

et al. 2022

Front. Bioeng. Biotechnol.

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Cardiac fibrosis is a common pathology in cardiovascular diseases which are reported as the leading cause of death globally. In recent decades, accumulating evidence has shown that the biomechanical traits of fibrosis play important roles in cardiac fibrosis initiation, progression and treatment. In this review, we summarize the four main distinct biomechanical traits (i.e., stretch, fluid shear stress, ECM microarchitecture, and ECM stiffness) and categorize them into two different types (i.e., static and dynamic), mainly consulting the unique characteristic of the heart. Moreover, we also provide a comprehensive overview of the effect of different biomechanical traits on cardiac fibrosis, their transduction mechanisms, and in-vitro engineered models targeting biomechanical traits that will aid the identification and prediction of mechano-based therapeutic targets to ameliorate cardiac fibrosis.

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