Biomass production and secondary metabolite identification in callus cultures of Coryphantha macromeris (Engelm.) Britton & Rose (Cactaceae), a traditional medicinal plant

Callus induction and phytochemical profiling of Yucca carnerosana (Trel.) McKelvey obtained from in vitro cultures

“…Additionally, three piscidic acid isomers (compounds 13, 21, and 22) were detected in plants growing under in vitro and ex vitro conditions and assigned as reported previously (Cabañas-García et al, 2019), see Table 2. Compounds 15, 17, 18, and 50 were mainly detected in callus tissue and were identified as rhynchosporoside isomers as reported previously for callus cultures of Coryphantha macromeris (Cactaceae) (Cabañas-García et al, 2021). Similarly, compounds 19 and 23 were detected in callus tissue, and identified as 2-O-(2-hydroxyethyl)-4-O-[2-O-(2-hydroxypropyl) hexopyranosyl] hexopyranose isomers.…”

“…For this metabolite, its therapeutic effect against UVB-induced photo-aging of the skin has been proposed (Jeon et al, 2019). Compound 45 was assigned as magnoloside U as reported previously (Cabañas-García et al, 2021), and compound 46 was assigned as 3,4,5-triacetyloxy-6-[(4-oxo-2,3-dihydro-cyclopentachromen-7-yl)oxy]oxan-2yl]methyl acetate since pseudomolecular ion at m/z: 531.1505 yielded one fragment at m/z: 134.0365, and compound 47 was proposed as the glycosylated flavonoid naringenin 7-O-rutinoside due to the presence of one main fragment at m/z: 266.0659, generated by the loss of the glycosidic moiety.…”

Production of secondary metabolites using tissue culture-based biotechnological applications

“…In addition, some active ingredients and secondary compounds have successfully been produced by application of tissue culture approach using intact plants ( Figure 4 ). The examples are as listed: phenolic molecules, including apigenin, p-coumaric acid, genistein, luteolin, rutin hydrate, trans-ferulic acid, salicylic acid and naringenin from Coryphantha macromeris ( Karakas and Bozat, 2020 ); medicinally vital phenolic and flavonoid compounds, including apigenin, caffeic acid, catechin, gallic acid, hederagenin, myricetin, kaempherol, isorhamnetin, nahagenin, ursolic acid, betulinic acid from Fagonia indica ( Khan et al., 2019 ); p-coumaric acid, hesperidin, cafeic acid, rosmarinic acid from Rosmarinus officinalis ( Coskun et al., 2019 ); phenolics, including gallic acid, chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, vanillic acid, luteolin and iso-rhamnetin, from Lycium barbarum ( Karakas, 2020 ); gingerol, shogaol, and zingerone from Zingiber officinale ( Arijanti and Suryaningsih, 2019 ); indole alkaloids, including echitamine, acetylechitamine, tubotaiwine and picrinine from Alstonia scholaris ( Jeet et al., 2020 ); crocin from Crocus sativus ( Moradi et al., 2018 ); anticancer alkaloids (vincristine and vinblastine) from Catharanthus roseus ( Mekky et al., 2018 ); phenylethanoid (salidroside, tyrosol), phenylpropanoid (rosavin and rosarin) and phenolic acids (p-coumaric acid, gallic acid, and cinnamic acid) from Rhodiola imbricata ( Rattan et al., 2020 ); eugenol and ursolic acid from Ocimum tenuiflorum ( Sharan et al., 2018 ); bioactive compounds, including 1,2-benzenedicarboxylic acid (phthalic acid), 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 2-hexadecen-1-ol-3,7,11,15-tetrametil, hexadecanoic acid methyl ester (methyl palmitate), n-hexadecanoic acid (palmitic acid), 9,12-octadecadienoic acid methyl ester, 9,12,15-octadecatrienoic acid methyl ester, phytol, octadecanoic acid methyl ester (methyl stearate), 9,12,15-octadecatrienoic acid (linolenic acid) and squalene from Mucuna pruriens ( Sweetlin and Daniel, 2020 ); several different metabolics, including acetamide, propanoic acid, α-thujene, linalool, 5-hydroxymethylfurfural, β-maaliene, epidolichodial, calarene, seychellene, α-curcumene, eremophilene, α-vatirenene, valencene, α-cadinol, ledol, meso-erythritol, α-gurjunene, viridiflorol, (-)-globulol, spirojatamol, dodecanoic acid, patchouli alcohol, jatamansone, xylitol, aristolone, protocatechuic acid, mannose, hexadecanoic acid, p-coumaric acid, talose, α-D-mannopyranose, α-D-galactopyranoside, D-mannitol, myo-inositol, -D-glucopyranoside, D-(+)-trehalose, D-(+)-cellobiose, melibiose, vitamin E, β-sitosterol from Nardostachys jatamansi ( Bose et al., 2019 ); identified 11 organic acids, 16 phenolic acids, 8 flavonoids, and 17 metabolites of different classes from Coryphantha macromeris ( Cabanas-Garcia et al., 2021 ); phenolic compounds (ferulic acid, isoquercitrin, rutin, quercetin, quercetin-7-O-glucoside and luteolin) from Hyssopus officinalis ( Babich et al., 2021 ) and phenylethanoids and st...…”

Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

“…The suspension cell systems of plants, a potential way to produce various plant active substances, combines the advantages of others and can also be used as suitable materials for studying complicated metabolic courses and biosyntheses. 166 Many active substances of plants are valuable for research in applications, and it can be predicted that the demand for them will increase in the future, so it is necessary to develop technologies that can produce a large number of useful active substances of plants in a sustainable way. 167 Adding a little pectinase avoids cell aggregations during a conventional shaking table culture and forms a uniform cells suspension 168 for better function.…”