Biomarkers of free radical injury during spinal cord ischemia

Basu, Samar; Hellberg, Anders; Ulus, A. Tulga; Westman, Jan; Karacagil, Sadettin

doi:10.1016/s0014-5793(01)02998-2

Cited by 45 publications

(23 citation statements)

References 20 publications

(27 reference statements)

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“…8-Iso-prostaglandin F 2a is an isoprostane that has been shown to be useful for assessing oxidative stress in spinal cord ischemia. 49 It is a biologically stable end-product of the free radical-induced oxidation of arachidonic acid produced from non-cyclooxygenase and cyclooxygenase peroxidation pathways. It appears in normal plasma and urine samples and is elevated by oxidative stress.…”

Section: Nucleusmentioning

confidence: 99%

“…In experimental spinal cord ischemia, a significant and immediate increase of 8-iso-prostaglandin F 2a in plasma at the start and up to 60 min, and in the urine at 90-50 min was observed, indicating a consequence of oxidative injury. 49 Compared with other assays, the greatest advantage of this immunoassay is that the level of 8-iso-prostaglandin F 2a is sufficient to be detected in every normal biological fluid, and the limit of detection is down to 23 pmol l À1 .…”

Section: Nucleusmentioning

confidence: 99%

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Oxidative stress in spinal cord injury and antioxidant-based intervention

Jia

Zhu

Li³

et al. 2011

Spinal Cord

245

159

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Study design: Literature review. Objectives: Spinal cord injury (SCI) remains a major public health issue in developed countries as well as worldwide. The pathophysiology of SCI is characterized by an initial primary injury followed by secondary deterioration. Although the etiology and pathogenesis of SCI remain to be fully understood, it has been suggested that reactive oxygen species (ROS) and oxidative stress have a significant role in the pathophysiology of SCI. Thus, alleviating oxidative stress may be an effective strategy for therapeutic intervention of SCI. The aim of this review was to describe (i) the sources of ROS as well as the major antioxidant defenses with particular attention being paid to lipid peroxidation; (ii) the biomarkers of oxidative stress in SCI and (iii) the neuroprotective effects of various compounds with antioxidative properties in animal models of SCI. Methods: PubMed, one of the most comprehensive biomedical databases, was searched from 1976-2011. All relevant papers were read by title, abstract and full-length article. Results: Oxidative stress is considered a hallmark of injury of SCI. Thus, alleviating oxidative stress may be an effective way of therapeutic intervention of SCI. Two of these agents, the glucocorticoid steroid methylprednisolone and the non-glucocorticoid 21-aminosteroid tirilazad, have been shown to possess significant antioxidant activities and improve recovery of SCI patients in clinical trials. Other promising botanical compounds and their molecular targets and mechanisms of action with regard to potential protection against SCI were also described. These include carotenoids and phenolic compounds. Conclusion: ROS and oxidative stress have a significant role in the pathophysiology of SCI. Alleviating oxidative stress is be an effective strategy for therapeutic intervention of SCI. Extensive research over the past several decades has identified numerous bioactive compounds that have antioxidative stress benefits in animal models of SCI. Thus, continued studies on bioactive compounds with ROS-scavenging capacity may lead to the development of effective antioxidant-based modalities for treating SCI in human subjects.

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Section: Nucleusmentioning

confidence: 99%

Section: Nucleusmentioning

confidence: 99%

Oxidative stress in spinal cord injury and antioxidant-based intervention

Jia

Zhu

Li³

et al. 2011

Spinal Cord

245

159

View full text Add to dashboard Cite

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“…The tritium labelled 8-iso-PGF 2a (specific activity: 608 GBq mmol 21 ) was synthesized and purified as described previously. [18] The tritium labelled 15-keto-dihydro-PGF 2a (specific activity: 6.77 TBq mmol 21 ) was obtained from Amersham (Buckinghamshire, UK).…”

Section: Chemicalsmentioning

confidence: 99%

“…[18] The tritium labelled 15-keto-dihydro-PGF 2a (specific activity: 6.77 TBq mmol 21 ) was obtained from Amersham (Buckinghamshire, UK). Antibodies against both 8-iso-PGF 2a and 15-keto-dihydro-PGF 2a were raised at our laboratory and are well characterized.…”

Section: Chemicalsmentioning

confidence: 99%

“…[17] We have developed radioimmunoassays through raising specific antibodies against both 8-iso-PGF 2a and 15-keto-dihydro-PGF 2a . [17,18] By quantifying these parameters, we have established that oxidative modification of arachidonic acid is associated with hepatotoxicity, [13] septic shock, [12] cardiac arrest and reperfusion, [7,19] various rheumatic diseases, [20] spinal cord ischemia, [21] reduced bone mineral density, [22] atherogenesis, [23] coronary bypass surgery [24] and is found also among post-menopausal women. [25] The aim of the present study was to investigate whether the total duration of cardiac arrest and CPR, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for Time-dependent Maximum Increase of Free Radical Damage and Eicosanoid Formation in the Brain as Related to Duration of Cardiac Arrest and Cardio-pulmonary Resuscitation

Basu

Liu

Nozari

et al. 2003

Free Radical Research

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Inhibitory effects of novel tetrahydropyridine derivatives on nitric oxide and reactive oxygen species production in glioma cells

Yoon

Redda

Mazzio

et al. 2004

Drug Development Research

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The immune response in the central nervous system involves the degeneration of neurological tissue. The therapeutic use of nonsteroidal anti-inflammatory agents (NSAIDs) can be effective in reducing inflammation and associated neurodegeneration. The current study was designed to examine the effects of previously synthesized N-(substituted benzoylamino)-4-ethyl-1,2,3,6-tetrahydropyridines (THPs) on free radical and nitric oxide (NO) generation in C6 glioma cells activated with bacterial endotoxin [lipopolysaccharide (LPS): Escherichia coli 0111:B4] and interferon-g. Stimulated C6 cells exhibited elevated iNOS protein expression, an increase of reactive oxygen species and NO 2 À . All parameters were attenuated significantly by indomethacin and various THPs (4-Ome, 3-CF3, 4-Me, 4-Et, 4-t-butyl, and 4-n-butyl). The results indicate a possible role for THP derivatives as anti-inflammatory agents. Drug Dev.

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Biomarkers of free radical injury during spinal cord ischemia

Cited by 45 publications

References 20 publications

Oxidative stress in spinal cord injury and antioxidant-based intervention

Oxidative stress in spinal cord injury and antioxidant-based intervention

Evidence for Time-dependent Maximum Increase of Free Radical Damage and Eicosanoid Formation in the Brain as Related to Duration of Cardiac Arrest and Cardio-pulmonary Resuscitation

Inhibitory effects of novel tetrahydropyridine derivatives on nitric oxide and reactive oxygen species production in glioma cells

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