Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs

Alam, Mahabub; Ji, Joong Ryong; Kim, Min Su; Kim, Nam Soo

doi:10.4142/jvs.2011.12.3.273

Cited by 29 publications

(36 citation statements)

References 24 publications

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“…Alam et al, 17 measured the levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of MMP (TIMP)-2 in synovial fluids and sera from dogs with OA. This study suggests that TRAP, MMP-2, and TIMP-2 in synovial fluid and serum may help to identify early phases of canine OA.…”

Section: Introductionmentioning

confidence: 99%

Osteoarthritis year 2012 in review: biomarkers

Mobasheri

2012

Osteoarthritis and Cartilage

106

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Novel OA biomarkers are needed for sub-clinical disease diagnosis. Proteomic techniques are beginning to yield useful data and deliver new OA biomarkers in serum and urine. Combining biochemical markers with tissue and cell imaging techniques and bioinformatics (i.e., machine learning, clustering, data visualization) may facilitate the development of biomarker combinations enabling earlier detection of OA.

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Section: Introductionmentioning

confidence: 99%

Osteoarthritis year 2012 in review: biomarkers

Mobasheri

2012

Osteoarthritis and Cartilage

106

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“…61 During the progression of OA induced by a surgically created medial patellar luxation (MPL) in the left stifle of dog, synovial fluid and serum TIMP-2 levels were significantly decreased, whereas MMP-2 in synovial fluid was significantly increased. 62 In dogs referred to the clinic, synovial fluid TIMP-1 level was increased in dogs with ACL rupture (ACLR), osteochondritis dissecans (OCD), fragmented coronoid process (FPC), and hip dysplasia (HD) compared to dogs without musculoskeletal diseases. 63 Carprofen (2.2 and 4.4 mg/kg), a nonsteroidal inflammatory drug, had no effect on MMP activity in ACLT-induced OA dogs.…”

Section: Dogsmentioning

confidence: 99%

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

et al. 2016

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Objective. Osteoarthritis (OA) is one of the leading causes of disability within the adult population. Currently, its diagnosis is mainly based on clinical examination and standard radiography. To date, there is no way to detect the disease at a molecular level, before the appearance of structural changes and symptoms. So an attractive alternative for monitoring OA is the measurement of biochemical markers in blood, urine, or synovial fluid, which could reflect metabolic changes in joint tissue and therefore disease onset and progression. Animal models are relevant to investigate the early stage of OA and metabolic changes occurring in joint tissues. The goal of this narrative review is to summarize the scientific data available in the literature on soluble biomarkers in animal models of OA. Design. A literature search was conducted using the PubMed/ Medline and Scopus databases between February 1995 and December 2015. All original articles, systematic and narrative reviews published in French or in English were considered. Results. We summarized the data of 69 studies and proposed a classification scheme for OA biomarkers in animal studies, largely inspired by the BIPEDS classification. Conclusions. Studies about biomarkers and animal models indicate that some markers could be valuable to monitor OA progression and assess therapeutic response in some animal models.

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“…Patellar luxation causes joint instability from the lateral or medial movement of the patella on the femoral groove. The movement of the patella in and out of the femoral groove in PL can cause cartilage erosion [19][20][21] , which may then develop into OA [18,22,23] .…”

Section: Introductionmentioning

confidence: 99%

Köpeklerde Patellar Luksasyonda Kıkırdak Erozyonunun Prevalansı ve Etkilenmiş Eklemlerdeki Gen Ekspresyonu

Boonsri¹,

Pradit²,

Soontornvipart³

et al. 2016

Kafkas Univ Vet Fak Derg

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The objectives of this study were to assess the prevalence of cartilage erosion in small dogs with patellar luxation (PL), and related osteoarthritis (OA)-related gene expression. In Study 1, 71 dogs were examined to determine risk factors associated with PL, including breed, age, weight, sex, and affected joint. In Study 2, a total of 39 dogs were divided into four groups: normal articular cartilage in the stifle joint (G1; n=5); PL without cartilage erosion (G2; n=11); PL with cartilage erosion (G3; n=14); and OA in the stifle (G4; n=9). Articular cartilage and synovial membranes were collected during surgical operations to correct PL. Real-time PCR was used to quantify the expression levels of 11 OA-related genes, including AGG, COL2A1, HAS-1, with GAPDH used as a reference gene. From Study 1, it was found that the risk factors related with cartilage erosion lesion were age, sex, and PL grade (all variables showed P<0.05). From Study 2, it was demonstrated that PL with or without cartilage erosion expressed pro-inflammatory cytokines and enzymes; some biomolecules were up regulated (IL-1β, MMP-3, AGG, TIMP-1) but some were down regulated (COL2A1, . This expression was the difference between the articular cartilage and the synovial membrane; however, the expression of genes from PL with cartilage erosion was observed to be similar to that of OA. From our results, it can be concluded that PL can develop into secondary OA due to an increase of IL-1β in cartilage and synovial membrane. Keywords: Cartilage erosion, Dog, Gene expression, Patellar luxation Köpeklerde Patellar Luksasyonda Kıkırdak Erozyonunun Prevalansı ve Etkilenmiş Eklemlerdeki Gen Ekspresyonu ÖzetBu çalışmanın amacı patellar luksasyonlu (PL) küçük cüsseli köpeklerde kartilaj erozyonunun prevalansını ve osteoartritis (OA)-alakalı gen ekspresyonunu belirlemektedir. Birinci araştırmada; cins, yaş, cinsiyet ve infekte eklemi içeren PL ile ilgili risk faktörlerini belirlemek amacıyla 71 köpek incelendi. İkinci araştırmada toplam 39 köpek dört gruba ayrıldı; diz ekleminde normal artikular kartilaj (G1; n=5), kartilaj erozyon olmayan PL (G2; n=11), kartilaj erozyonlu PL (G3; n=14) ve dizde OA (G4; n=9). Cerrahi operasyon sırasında PL'u düzeltmek amacıyla artiküler kartilaj ve sinoviyal zarlar alındı. Toplam 11 adet OA ile ilgili genin (AGG, COL2A1,; referans gen olarak GAPDH kullanıldı) ekspresyon düzeylerini belirlemek amacıyla PCR tekniği uygulandı. Birinci araştırmanın sonucunda yaş, cinsiyet ve PL derecesi kartilaj erozyonu ile ilgili risk faktörleri olarak belirlendi (P<0.05). İkinci araştırmada kartilaj erozyonlu veya erozyon bulunmayan PL'lu köpeklerde pro-inflamatuar sitokinleri eksprese ettikleri, bazı biyomoleküllerin ekspresyonunu artırdıkları AGG, bazılarını ise azalttıkları (IL-1β, MMP-3, AGG, TIMP-1) gözlemlendi. Bu ekspresyon artiküler kartilaj ve sinoviyal zar için farklıydı. Ancak kartilaj erozyonlu PL'da genlerin ekspresyonu OA ile benzerlik göstermekteydi. Çalışma bulguları doğrultusunda kıkırdak ve sinoviyal zarlarda artan ...

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Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs

Cited by 29 publications

References 24 publications

Osteoarthritis year 2012 in review: biomarkers

Osteoarthritis year 2012 in review: biomarkers

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

Köpeklerde Patellar Luksasyonda Kıkırdak Erozyonunun Prevalansı ve Etkilenmiş Eklemlerdeki Gen Ekspresyonu

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