Bioluminescent Nano- and Micro-biosensing Elements for Detection of Organophosphorus Compounds

Ефременко, Елена; Lyagin, Ilya; Senko, Olga; Maslova, Olga; Stepanov, Nikolay

doi:10.1007/978-3-030-55490-3_14

Cited by 1 publication

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“…Для усиления первичного аналитического сигнала в рекомбинантных клетках микроорганизмов часто дополнительно повышают уровень биосинтеза фермента, необходимого для детекции ФОС [99][100][101][102][103][104][105][106]. В то же время методы генетической инженерии позволяют точно управлять локализацией фермента в клетке в клеточной стенке, периплазме, цитоплазме после экспрессии соответствующего гена.…”

Section: химическая безопасность и защита от химического терроризмаunclassified

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

2021

Journal of NBC Protection Corps

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Еnzymes are able to effectively interact with various organophosphorus compounds (OPC), entering into (bio)chemical reactions with them. Changes in the initial activity of enzymes as a result of their inhibition by OPC, the formation of OPC degradation products under the action of hydrolytic enzymes, etc. can be determined using different physical and chemical methods and used in bioanalytic systems to determine the concentrations of OPC. The purpose of the review is to analyze the main scientific results achieved over the past 10 years in the development of analytical systems based on enzymes intended for the determination of OPC. It is shown in the article, that the requirements for the sensitivity of biosensors are based on the norms of the content of the analyzed substances detected in/at the objects of mandatory control. The cholinesterases compose a basis for the development of the largest number of ultra-sensitive biosensors, although other enzymes can be successfully used as a biosensitive element. The most technologically advanced solution that is close to the practical implementation seems to be bioanalytical systems using immobilized enzymes. Improving the detection limits of the OPC can be achieved by using nanoobjects together with modern methods of signal transducers, for example, with nanomechanical detectors and signal converters. This combination of technical solutions ensures the sensitivity of the OPC analysis up to pg/l. At present, «reagentless» systems have received significant development, which have become the basis for the production of a large number of commercially available strips for the express determination of OPC. Modern demands stimulate the rapid development of portable and, especially, wearable biosensors that can be attached to various surfaces, including a clothing. The progress in the development of affine amino acid sequences, in the future, will allow the creation of enzyme biosensors on any surface.

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Section: химическая безопасность и защита от химического терроризмаunclassified

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

2021

Journal of NBC Protection Corps

View full text Add to dashboard Cite

show abstract

Bioluminescent Nano- and Micro-biosensing Elements for Detection of Organophosphorus Compounds

Cited by 1 publication

References 97 publications

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

Enzymes and Their Forms Used in Detection of Organophosphorus Compounds

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