Biologically synthesized silver nanoparticles as potent antibacterial effective against multidrug-resistant Pseudomonas aeruginosa

Abstract

Significance and Impact of the Study: The continuous rise of antibiotic resistance in bacteria such as Pseudomonas aeruginosa is a serious threat to human health, hence it is mandatory to identify novel ways to combat its infections. In this work, silver nanoparticles were synthesized in a green way using leaves and fugal extracts and its antibacterial effects were tested against clinical strains. The nanoparticles killed the strains due severe cellular damage affecting the membranes, the DNA and generating reactive oxygen species, in addition they were nontoxic and displayed synergy with some antibiotics; therefore, being a promising alternative to treat MDR P. aeruginosa infections.

Synthesis and Characterization of Bacterial Cellulose‐Curcumin‐Ag‐Se Nanocomposites having Antioxidant and Antibacterial properties for Skin Healing Applications

“…AgNPs bind to the cell membrane thereby rupturing it and causing leakage of cellular material leading to cell death. [54] In a study done by Beleno et al [55] when MDR P. aeruginosa was treated with AgNPs, it showed significant damage to the cell with accumulation of AgNPs in the cell wall resulting in a crenated cell wall, disrupted plasma membrane and a decreased electron density of cytoplasm suggesting leakage of cellular material. Secondly, silver ions bind to phosphorus residues in DNA causing inactivation and compromised fidelity of DNA replication.…”

Silver Nanoparticle-Based Therapy: Can It Be Useful to Combat Multi-Drug Resistant Bacteria?

“…Therefore, AgNPs exhibit a remarkable antibacterial activity against MDR P. aeruginosa , representing a possible alternative for antibiotics and they can also be promising antibiofilm agents. The systemic administration of these particles seems at this time difficult to implement owing to the possible accumulation and damage of tissues and organs (toxicity); however, either coating of prosthetic devices/catheters or their topical application for the treatment of skin infections and the prevention of disease in burnt patients may be a future application of AgNPs [ 99 , 100 ]. Therefore, the utility of AgNPs against this bacterium has been evidenced.…”

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

“…aerogenes [16,[33][34][35][36][37]. Although the precise mechanism remains unclear, studies suggest that AgNPs impact the bacterial cell membrane and interfere with ribosome and protein synthesis.…”