Biological significance of cancer-associated sialyl-Tn antigen: Modulation of malignant phenotype in gastric carcinoma cells

Pinho, Sandra; Marcos, Nuno T.; Ferreira, Bibiana I.; Carvalho, Ana Sofía; Oliveira, Maria José; Santos-Silva, Filipe; Harduin-Lepers, Anne; Reis, Celso A.

doi:10.1016/j.canlet.2006.08.010

Cited by 136 publications

(119 citation statements)

References 59 publications

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“…Procedures and mAb references have been previously described (50). mAb anti-sialyl-Le x , clone KM93, was purchased from Chemicon International and used according to the same protocol at a 1:40 dilution.…”

Section: Bacteria and Gastric Cell Lines Human Gastric Carcinoma Celmentioning

confidence: 99%

Helicobacter pylori induces β3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl–Lewis x

Marcos¹,

Magalhães²,

Ferreira³

et al. 2008

J. Clin. Invest.

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Section: Bacteria and Gastric Cell Lines Human Gastric Carcinoma Celmentioning

confidence: 99%

Helicobacter pylori induces β3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl–Lewis x

Marcos¹,

Magalhães²,

Ferreira³

et al. 2008

J. Clin. Invest.

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“…Pinho et al [32], reported that forced expression of STn leads to major morphological and cell behavior alterations in gastric carcinoma cells that were reversible by specific antibody blockade. STn antigen is able to modulate a malignant phenotype and induce a more aggressive cell behavior, such as decreased cell-cell aggregation and increased extracellular matrix (ECM) adhesion, migration, and invasion.…”

Section: Introductionmentioning

confidence: 99%

RNAi-mediated gene silencing of ST6GalNAc I suppresses the metastatic potential in gastric cancer cells

et al. 2014

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Background ST6GalNAc I is a sialyltransferase controlling the expression of sialyl-Tn antigen (STn), which is overexpressed in several epithelial cancers, including gastric cancer, and is highly correlated with cancer metastasis. However, the functional contribution of ST6GalNAc I to development or progression of gastric cancer remains unclear. In this study, we investigated the effects of suppression of ST6GalNAc I on gastric cancer in vitro and in vivo. Methods Gastric cancer cell lines were transfected with ST6GalNAc I siRNA and were examined by cell proliferation, migration, and invasion assays. We also evaluated the effect of ST6GalNAc I siRNA treatment in a peritoneal dissemination mouse model. The differences in mRNA levels of selected signaling molecules were analyzed by polymerase chain reaction (PCR) arrays associated with tumor metastasis in MKN45 cells. The signal transducer and activator of transcription 5b (STAT5b) signaling pathways that reportedly regulate the insulin-like growth factor-1 (IGF-1) were analyzed by Western blot.

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“…STn and MUC1 mucin, its most extensively studied carrier in cancer cells, have served as target for cancer immunotherapy in preclinical and clinical studies. 9 CD24 is highly glycosylated and contains three potential N-glycosylation sites and several potential O-glycosylation sites. Recently, there were two reports in which the detailed structures of N-and O-glycans from mouse brain CD24 have been reported.…”

mentioning

confidence: 99%

Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis

Kristiansen

Machado

Bretz

et al. 2010

Laboratory Investigation

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CD24 is a small, highly glycosylated cell surface protein that is linked to the membrane through a glycosyl-phosphatidylinositol anchor. It is overexpressed in many human carcinomas and its expression is linked to bad prognosis. Lately, lack or low expression of CD24 was used to identify tumor stem cells resulting in conflicting data on the usefulness of this marker. In many immunohistochemical studies, the mAb SN3b was used but the epitope and specificity of this antibody have never been thoroughly investigated. In other studies based mainly on cytofluorographic analysis, the mAb ML-5 was applied. In this study, we compared the epitope of mAb SN3b to the CD24 mAbs SWA-11 and ML-5 that both bind to the core protein of CD24. Using tissue microarrays and affinity-purified CD24 glycoforms, we observed only a partial overlap of SN3b and SWA11 reactivity. The mAb SN3b recognizes sialic acid most likely on O-linked glycans that can occur independently of the CD24 protein backbone. The SN3b epitope was not related to common sialylated cancer-associated glycan structures. Both SN3b epitope positive or negative CD24 glycoforms supported the binding of P-selectin and Siglec-5. In breast cancer, the SN3b reactivity was associated with bad prognosis, whereas SWA11 was not. In renal cell cancer, the SN3b epitope was completely absent but SWA11 reactivity was a prognostic factor. Our results shed new light on the tumorbiological role of CD24 and resolve discrepancies in the literature related to the use of different CD24 mAbs.

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Biological significance of cancer-associated sialyl-Tn antigen: Modulation of malignant phenotype in gastric carcinoma cells

Cited by 136 publications

References 59 publications

Helicobacter pylori induces β3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl–Lewis x

Helicobacter pylori induces β3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl–Lewis x

RNAi-mediated gene silencing of ST6GalNAc I suppresses the metastatic potential in gastric cancer cells

Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis

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